PMID- 29407217
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181105
IS  - 1873-7064 (Electronic)
IS  - 0028-3908 (Linking)
VI  - 133
DP  - 2018 May 1
TI  - The role of 19S proteasome associated deubiquitinases in activity-dependent 
      hippocampal synaptic plasticity.
PG  - 354-365
LID - S0028-3908(18)30049-2 [pii]
LID - 10.1016/j.neuropharm.2018.01.043 [doi]
AB  - Posttranslational modification and degradation of proteins by the 
      ubiquitin-proteasome system (UPS) is crucial to synaptic transmission. It is well 
      established that 19S proteasome associated deubiquitinases (DUBs) reverse the 
      process of ubiquitination by removing ubiquitin from their substrates. However, 
      their potential contribution to hippocampal synaptic plasticity has not been 
      addressed in detail. Here, we report that inhibition of the 19S proteasome 
      associated DUBs, ubiquitin C-terminal hydrolase 5 (UCHL5) and ubiquitin-specific 
      peptidase 14 (USP14) by b-AP15 results in an accumulation of polyubiquitinated 
      proteins and a reduction of monomeric ubiquitin without overt effects on 26S 
      proteasome activity. b-AP15 led to a suppression of mTOR-p70S6K signaling and an 
      increase in levels of p-p38 MAPK, two pathways essentially involved in 
      establishing various forms of activity-dependent plasticity. Additionally, b-AP15 
      impaired the induction of late-phase long-term potentiation (L-LTP), induced the 
      transformation of mGluR-mediated protein synthesis-independent long-term 
      depression (early-LTD) to L-LTD and promoted heterosynaptic stabilization through 
      synaptic tagging/capture (STC) in the hippocampal CA1 region of mice. The 
      activity of 19S proteasome associated DUBs was also required for the enhancement 
      of short-term potentiation (STP) induced by brain-derived neurotrophic factor 
      (BDNF). Altogether, these results indicate an essential role of 19S proteasome 
      associated DUBs in regulating activity-dependent hippocampal synaptic plasticity.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Yun, Di
AU  - Yun D
AD  - Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and 
      Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, 
      China.
FAU - Zhuang, Yinghan
AU  - Zhuang Y
AD  - Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and 
      Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, 
      China.
FAU - Kreutz, Michael R
AU  - Kreutz MR
AD  - Research Group Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, 
      Germany; Leibniz Group "Dendritic Organelles and Synaptic Function", Center for 
      Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 
      Hamburg, Germany.
FAU - Behnisch, Thomas
AU  - Behnisch T
AD  - Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and 
      Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, 
      China. Electronic address: behnish@fudan.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180131
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (3,5-bis((4-nitrophenyl)methylidene)-1-prop-2-enoylpiperidin-4-one)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Piperidones)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Ubiquitin)
RN  - EC 3.4.19.12 (Deubiquitinating Enzymes)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Line, Transformed
MH  - Deubiquitinating Enzymes/*metabolism
MH  - Electric Stimulation
MH  - Excitatory Postsynaptic Potentials/drug effects/physiology
MH  - Hippocampus/*cytology
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuronal Plasticity/drug effects/*physiology
MH  - Patch-Clamp Techniques
MH  - Piperidones/pharmacology
MH  - Protease Inhibitors/pharmacology
MH  - Proteasome Endopeptidase Complex/*metabolism
MH  - Transfection
MH  - Ubiquitin/*metabolism
MH  - Ubiquitination/drug effects
OTO - NOTNLM
OT  - 19S proteasome
OT  - BDNF
OT  - Deubiquitinases
OT  - Synaptic plasticity
OT  - Synaptic tagging
EDAT- 2018/02/07 06:00
MHDA- 2018/11/06 06:00
CRDT- 2018/02/07 06:00
PHST- 2017/08/18 00:00 [received]
PHST- 2017/12/24 00:00 [revised]
PHST- 2018/01/29 00:00 [accepted]
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/02/07 06:00 [entrez]
AID - S0028-3908(18)30049-2 [pii]
AID - 10.1016/j.neuropharm.2018.01.043 [doi]
PST - ppublish
SO  - Neuropharmacology. 2018 May 1;133:354-365. doi: 10.1016/j.neuropharm.2018.01.043. 
      Epub 2018 Jan 31.