PMID- 29407749
OWN - NLM
STAT- MEDLINE
DCOM- 20181005
LR  - 20201209
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 88
DP  - 2018 Apr
TI  - Differentiation of stem cells from human deciduous and permanent teeth into 
      spiral ganglion neuron-like cells.
PG  - 34-41
LID - S0003-9969(18)30019-0 [pii]
LID - 10.1016/j.archoralbio.2018.01.011 [doi]
AB  - OBJECTIVE: Stem cells from pulp tissue are a promising cell-based therapy for 
      neurodegenerative patients based on their origin in the neural crest. The aim of 
      this study was to differentiate and evaluate the ability of human dental pulp 
      stem cells from permanent teeth (DPSC) and stem cells from human exfoliated 
      deciduous teeth (SHED) to differentiate into spiral ganglion neurons. DESIGN: 
      After isolation and characterization of mesenchymal stem cell properties, DPSC 
      and SHED were treated with the neurotrophins brain-derived neurotrophic factor 
      (BDNF), neurotrophin-3 (NT-3), and glial cell-derived neurotrophic factor (GDNF). 
      The differentiation was identified by immunostaining and qRT-PCR analysis of 
      neuronal markers and measuring intracellular calcium activity. RESULTS: After 2 
      weeks of induction, morphological changes were observed in both DPSC and SHED. 
      The differentiated cells expressed neuron-specific class III beta-tubulin, GATA 
      binding protein 3 (GATA3) and tropomyosin receptor kinase B, protein markers of 
      spiral ganglion neurons. These cells also showed upregulation of the genes 
      encoding these proteins, namely GATA3 and neurotrophic receptor tyrosine kinase 
      2. Intracellular calcium dynamics that reflect neurotransmitter release were 
      observed in differentiated DPSC and SHED. CONCLUSION: These results demonstrate 
      that dental pulp stem cells from permanent and deciduous teeth can differentiate 
      into spiral ganglion neuron-like cells.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Gonmanee, Thanasup
AU  - Gonmanee T
AD  - Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand.
FAU - Thonabulsombat, Charoensri
AU  - Thonabulsombat C
AD  - Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand.
FAU - Vongsavan, Kutkao
AU  - Vongsavan K
AD  - Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, 
      Bangkok, Thailand.
FAU - Sritanaudomchai, Hathaitip
AU  - Sritanaudomchai H
AD  - Department of Oral Biology, Faculty of Dentistry, Mahidol University, 6 Yothi 
      Road, Rajthawee, Bangkok, 10400, Thailand. Electronic address: 
      hathaitip.sri@mahidol.ac.th.
LA  - eng
PT  - Journal Article
DEP - 20180202
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Antigens, Surface)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NTF3 protein, human)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (TUBB3 protein, human)
RN  - 0 (Tubulin)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (tropomyosin-related kinase-B, human)
SB  - IM
MH  - Antigens, Surface/analysis/metabolism
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Differentiation/*drug effects/genetics
MH  - Cell Plasticity
MH  - Dental Pulp/*cytology
MH  - *Dentition, Permanent
MH  - Fibroblasts/cytology
MH  - GATA3 Transcription Factor/genetics/metabolism
MH  - Gene Expression
MH  - Glial Cell Line-Derived Neurotrophic Factor/pharmacology
MH  - Hearing Loss/therapy
MH  - Humans
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Nerve Growth Factors/pharmacology
MH  - Neurons/*cytology
MH  - Neurotrophin 3
MH  - Receptor, trkB/genetics/metabolism
MH  - Spiral Ganglion/*cytology/*metabolism
MH  - Tooth, Deciduous/*cytology
MH  - Tubulin/genetics/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Dental pulp stem cells
OT  - Neurotrophins
OT  - Spiral ganglion neuron
OT  - Stem cells from human exfoliated deciduous teeth (SHED)
EDAT- 2018/02/07 06:00
MHDA- 2018/10/06 06:00
CRDT- 2018/02/07 06:00
PHST- 2017/10/11 00:00 [received]
PHST- 2018/01/08 00:00 [revised]
PHST- 2018/01/16 00:00 [accepted]
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2018/10/06 06:00 [medline]
PHST- 2018/02/07 06:00 [entrez]
AID - S0003-9969(18)30019-0 [pii]
AID - 10.1016/j.archoralbio.2018.01.011 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2018 Apr;88:34-41. doi: 10.1016/j.archoralbio.2018.01.011. Epub 
      2018 Feb 2.