PMID- 29408024
OWN - NLM
STAT- MEDLINE
DCOM- 20181120
LR  - 20181120
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 214
DP  - 2018 Feb
TI  - Detection of porcine reproductive and respiratory syndrome virus (PRRSV)-specific 
      IgM-IgA in oral fluid samples reveals PRRSV infection in the presence of maternal 
      antibody.
PG  - 13-20
LID - S0378-1135(17)31114-8 [pii]
LID - 10.1016/j.vetmic.2017.11.011 [doi]
AB  - The ontogeny of PRRSV antibody in oral fluids has been described using 
      isotype-specific ELISAs. Mirroring the serum response, IgM appears in oral fluid 
      by 7days post inoculation (DPI), IgA after 7 DPI, and IgG by 9 to 10 DPI. 
      Commercial PRRSV ELISAs target the detection of IgG because the higher 
      concentration of IgG relative to other isotypes provides the best diagnostic 
      discrimination. Oral fluids are increasingly used for PRRSV surveillance in 
      commercial herds, but in younger pigs, a positive ELISA result may be due either 
      to maternal antibody or to antibody produced by the pigs in response to 
      infection. To address this issue, a combined IgM-IgA PRRSV oral fluid ELISA was 
      developed and evaluated for its capacity to detect pig-derived PRRSV antibody in 
      the presence of maternal antibody. Two longitudinal studies were conducted. In 
      Study 1 (modified-live PRRS vaccinated pigs), testing of individual pig oral 
      fluid samples by isotype-specific ELISAs demonstrated that the combined IgM-IgA 
      PRRSV ELISA provided better discrimination than individual IgM or IgA ELISAs. In 
      Study 2 (field data), testing of pen-based oral fluid samples confirmed the 
      findings in Study 1 and established that the IgM-IgA ELISA was able to detect 
      antibody produced by pigs in response to wild-type PRRSV infection, despite the 
      presence of maternal IgG. Overall, the combined PRRSV IgM-IgA oral fluid ELISA 
      described in this study is a potential tool for PRRSV surveillance, particularly 
      in populations of growing pigs originating from PRRSV-positive or vaccinated 
      breeding herds.
CI  - Copyright (c) 2017 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Rotolo, Marisa L
AU  - Rotolo ML
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA. Electronic address: mrotolo@iastate.edu.
FAU - Gimenez-Lirola, Luis
AU  - Gimenez-Lirola L
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Ji, Ju
AU  - Ji J
AD  - Department of Statistics, College of Liberal Arts and Sciences, Iowa State 
      University, Ames, IA, USA.
FAU - Magtoto, Ronaldo
AU  - Magtoto R
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Henao-Diaz, Yuly A
AU  - Henao-Diaz YA
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Wang, Chong
AU  - Wang C
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA; Department of Statistics, College of Liberal Arts and 
      Sciences, Iowa State University, Ames, IA, USA.
FAU - Baum, David H
AU  - Baum DH
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Harmon, Karen M
AU  - Harmon KM
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Main, Rodger G
AU  - Main RG
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Zimmerman, Jeffrey J
AU  - Zimmerman JJ
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
LA  - eng
PT  - Journal Article
DEP - 20171117
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - *Immunity, Maternally-Acquired
MH  - Immunoglobulin A/*analysis
MH  - Immunoglobulin M/*analysis
MH  - Longitudinal Studies
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Saliva/*immunology/virology
MH  - Swine
OTO - NOTNLM
OT  - ELISA
OT  - IgM-IgA
OT  - Oral fluid
OT  - PRRSV
OT  - Surveillance
EDAT- 2018/02/07 06:00
MHDA- 2018/11/21 06:00
CRDT- 2018/02/07 06:00
PHST- 2017/09/25 00:00 [received]
PHST- 2017/11/09 00:00 [revised]
PHST- 2017/11/10 00:00 [accepted]
PHST- 2018/02/07 06:00 [entrez]
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2018/11/21 06:00 [medline]
AID - S0378-1135(17)31114-8 [pii]
AID - 10.1016/j.vetmic.2017.11.011 [doi]
PST - ppublish
SO  - Vet Microbiol. 2018 Feb;214:13-20. doi: 10.1016/j.vetmic.2017.11.011. Epub 2017 
      Nov 17.