PMID- 29408442
OWN - NLM
STAT- MEDLINE
DCOM- 20180920
LR  - 20190329
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 248
DP  - 2018 Mar 15
TI  - Porcine reproductive and respiratory syndrome virus neutralizing antibodies 
      provide in vivo cross-protection to PRRSV1 and PRRSV2 viral challenge.
PG  - 13-23
LID - S0168-1702(17)30933-4 [pii]
LID - 10.1016/j.virusres.2018.01.015 [doi]
AB  - Vaccine control and prevention of porcine reproductive and respiratory syndrome 
      (PRRS), the most important disease of swine, is difficult to achieve. However, 
      the discovery of broadly neutralizing antibody activity against porcine 
      reproductive and respiratory syndrome virus (PRRSV) under typical field 
      conditions opens the door to new immunologic approaches for robust protection. We 
      show here that passive administration of purified immunoglobulins with 
      neutralizing antibodies reduced PRRSV2 infection by up to 96%, and PRRSV1 
      infection by up to 87%, whereas immune immunoglobulins lacking neutralizing 
      activity had no effect on viral infection. Hence, immune competence of passive 
      immunoglobulin transfer was associated specifically with antibody neutralizing 
      activity. Current models of PRRSV infection implicate a minor envelope 
      glycoprotein (GP) complex including GP2, GP3, and GP4, as critical to permissive 
      cell infection. However, conserved peptides comprising the putative cell 
      attachment structure did not attenuate neutralization or viral infection. The 
      results show that immunological approaches aimed at induction of broadly 
      neutralizing antibodies may substantially enhance immune protection against 
      PRRSV. The findings further show that naturally occurring viral isolates are able 
      to induce protective humoral immunity against unrelated PRRSV challenge, thus 
      removing a major conceptual barrier to vaccine development.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Robinson, Sally R
AU  - Robinson SR
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, St. 
      Paul, MN, USA.
FAU - Rahe, Michael C
AU  - Rahe MC
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, St. 
      Paul, MN, USA.
FAU - Gray, Diem K
AU  - Gray DK
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, St. 
      Paul, MN, USA.
FAU - Martins, Kyra V
AU  - Martins KV
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, St. 
      Paul, MN, USA.
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, St. 
      Paul, MN, USA. Electronic address: murta001@umn.edu.
LA  - eng
GR  - T32 RR018719/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180203
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/*immunology
MH  - Antibodies, Viral/*immunology
MH  - Cross Protection/*immunology
MH  - Genotype
MH  - Immunity, Humoral
MH  - Immunization, Passive
MH  - Immunoglobulin G/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & 
      control/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/genetics/*immunology
MH  - Swine
MH  - Viral Proteins/immunology
PMC - PMC5857471
MID - NIHMS945997
OTO - NOTNLM
OT  - Cross-protection
OT  - Neutralizing antibodies
OT  - Neutralizing epitope
OT  - PRRSV
OT  - Passive transfer
OT  - Swine
COIS- The authors declare no conflicts of interest.
EDAT- 2018/02/07 06:00
MHDA- 2018/09/21 06:00
PMCR- 2019/03/15
CRDT- 2018/02/07 06:00
PHST- 2017/12/20 00:00 [received]
PHST- 2018/01/27 00:00 [revised]
PHST- 2018/01/29 00:00 [accepted]
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2018/09/21 06:00 [medline]
PHST- 2018/02/07 06:00 [entrez]
PHST- 2019/03/15 00:00 [pmc-release]
AID - S0168-1702(17)30933-4 [pii]
AID - 10.1016/j.virusres.2018.01.015 [doi]
PST - ppublish
SO  - Virus Res. 2018 Mar 15;248:13-23. doi: 10.1016/j.virusres.2018.01.015. Epub 2018 
      Feb 3.