PMID- 29408682
OWN - NLM
STAT- MEDLINE
DCOM- 20190419
LR  - 20190419
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1684
DP  - 2018 Apr 1
TI  - Neurochemical changes in unilateral cerebral hemisphere during the subacute stage 
      of focal cerebral ischemia-reperfusion in rats: An ex vivo (1)H magnetic 
      resonance spectroscopy study.
PG  - 67-74
LID - S0006-8993(18)30040-4 [pii]
LID - 10.1016/j.brainres.2018.01.026 [doi]
AB  - Understanding the subacute may shed light on the mechanism of cerebral ischemia. 
      The present study aimed to explore metabolic features underlying subacute stage 
      of ischemia-reperfusion injury and developing effective treatments. Rats were 
      divided into three groups: the permanent middle cerebral artery occlusion 
      (pMCAO), transient cerebral focal ischemia (tMCAO) and sham group. Evaluation of 
      animal models was performed by the neurological deficit, MR images and 
      pathological morphological abnormality. To elucidate metabolic changes, we 
      conducted a comparative analysis of metabolic composition of unilateral brain 
      tissue using (1)H nuclear magnetic resonance spectroscopy. The successful model 
      was observed low signal on T1WI and high signal on T2WI lesions in the left 
      cerebral. Histopathological results confirmed the formation of apparent lesions 
      in the left striatum, hippocampus CA1 and cortex tissues of subacute cerebral 
      ischemia rats and showed that rats with focal cerebral ischemia-reperfusion could 
      alleviate the extent of pathological damage degree. In pMCAO rats 7 days after 
      surgery, decreased levels of N-acetyl aspartate (NAA), gamma-aminobutyric acid 
      (GABA), glutamate (Glu) and succinate (Suc) concomitantly with increased levels 
      of glutamine (Gln), myo-inositol (m-Ins) and lactate (Lac) were observed compared 
      to the control. Whereas, increased level of Lac with decreased levels of NAA, 
      GABA, Glu, Suc, creatine (Cre) were observed in the tMCAO rats. This demonstrated 
      that experimental subacute ischemic stroke in rats caused extensive perturbation 
      in energy metabolism, the tricarboxylic acid cycle and GABA shunt, which provided 
      essential information for understanding the pathogenesis of subacute cerebral 
      ischemia-reperfusion and provided guidance in choosing the suitable therapeutic 
      schedule.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Huang, Qun
AU  - Huang Q
AD  - Department of Radiology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325035, China; School of Pharmaceutical Sciences, Wenzhou 
      Medical University, Wenzhou 325035 China.
FAU - Li, Chen
AU  - Li C
AD  - School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035 
      China.
FAU - Xia, Nengzhi
AU  - Xia N
AD  - Department of Radiology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325035, China.
FAU - Zhao, Liangcai
AU  - Zhao L
AD  - School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035 
      China.
FAU - Wang, Dan
AU  - Wang D
AD  - School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035 
      China.
FAU - Yang, Yunjun
AU  - Yang Y
AD  - Department of Radiology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325035, China. Electronic address: yyj@hosp1.ac.cn.
FAU - Gao, Hongchang
AU  - Gao H
AD  - School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035 
      China. Electronic address: gaohc27@wmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180201
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
SB  - IM
MH  - Animals
MH  - Brain Ischemia/metabolism/*pathology
MH  - Energy Metabolism/physiology
MH  - Infarction, Middle Cerebral Artery/metabolism/*pathology
MH  - Ischemic Attack, Transient/metabolism/*pathology
MH  - *Magnetic Resonance Imaging/methods
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/metabolism/*pathology
OTO - NOTNLM
OT  - (1)H NMR
OT  - Metabolomics
OT  - Middle cerebral artery occlusion
OT  - Stroke
OT  - TCA cycle
EDAT- 2018/02/07 06:00
MHDA- 2019/04/20 06:00
CRDT- 2018/02/07 06:00
PHST- 2017/11/02 00:00 [received]
PHST- 2018/01/18 00:00 [revised]
PHST- 2018/01/19 00:00 [accepted]
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2019/04/20 06:00 [medline]
PHST- 2018/02/07 06:00 [entrez]
AID - S0006-8993(18)30040-4 [pii]
AID - 10.1016/j.brainres.2018.01.026 [doi]
PST - ppublish
SO  - Brain Res. 2018 Apr 1;1684:67-74. doi: 10.1016/j.brainres.2018.01.026. Epub 2018 
      Feb 1.