PMID- 29409377
OWN - NLM
STAT- MEDLINE
DCOM- 20180830
LR  - 20181113
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Print)
IS  - 1388-0209 (Linking)
VI  - 56
IP  - 1
DP  - 2018 Dec
TI  - The role of artichoke leaf tincture (Cynara scolymus) in the suppression of DNA 
      damage and atherosclerosis in rats fed an atherogenic diet.
PG  - 138-144
LID - 10.1080/13880209.2018.1434549 [doi]
AB  - CONTEXT: Polyphenols and flavonoids in artichoke leaf tincture (ALT) protect 
      cells against oxidative damage. OBJECTIVES: We examined ALT effects on 
      deoxyribonucleic acid (DNA) damage and lipid profiles in rat plasma and gene 
      expression in rat aorta [haemeoxygenase-1 (HO1), haemeoxygenase-2 (HO2), NADPH 
      oxidase 4 (NOX-4), monocyte chemoattractant protein-1 (MCP-1) and nuclear factor 
      (erythroid-derived 2)-like 2 (Nrf2)]. MATERIALS AND METHODS: Eighteen male Wistar 
      albino rats were divided into three groups (n = 6/group): The control group (CG) 
      was fed with standard pellet chow for 11 weeks; the AD group was fed for a 
      similar period of time with pellet chow supplemented with 2% cholesterol, 3% 
      sunflower oil and 1% sodium cholate. The ADA group was fed with pellet chow (for 
      1 week), the atherogenic diet (see above) for the following 4 weeks and then with 
      ALT (0.1 mL/kg body weight) and atherogenic diet for 6 weeks. According to HPLC 
      analysis, the isolated main compounds in ALT were chlorogenic acid, caffeic acid, 
      isoquercitrin and rutin. RESULTS: Normalized HO-1 [0.11 (0.04-0.24)] and MCP-1 
      [0.29 (0.21-0.47)] mRNA levels and DNA scores [12.50 (4.50-36.50)] were 
      significantly lower in the ADA group than in the AD group [0.84 (0.35-2.51)], 
      p = 0.021 for HO-1 [0.85 (0.61-3.45)], p = 0.047 for MCP-1 and [176.5 
      (66.50-221.25)], p = 0.020 for DNA scores. HO-1 mRNA was lower in the ADA group 
      than in the CG group [0.30 (0.21-0.71), p = 0.049]. CONCLUSIONS: Supplementation 
      with ALT limited the effects of the atherogenic diet through reduced MCP-1 
      expression, thereby preventing oxidative damage.
FAU - Bogavac-Stanojevic, Natasa
AU  - Bogavac-Stanojevic N
AD  - a Department of Medical Biochemistry, Faculty of Pharmacy , University of 
      Belgrade , Belgrade , Serbia.
FAU - Kotur Stevuljevic, Jelena
AU  - Kotur Stevuljevic J
AD  - a Department of Medical Biochemistry, Faculty of Pharmacy , University of 
      Belgrade , Belgrade , Serbia.
FAU - Cerne, Darko
AU  - Cerne D
AD  - b Department of Clinical Chemistry, Faculty of Pharmacy , University of Ljubljana 
      , Ljubljana , Slovenia.
FAU - Zupan, Janja
AU  - Zupan J
AD  - b Department of Clinical Chemistry, Faculty of Pharmacy , University of Ljubljana 
      , Ljubljana , Slovenia.
FAU - Marc, Janja
AU  - Marc J
AD  - b Department of Clinical Chemistry, Faculty of Pharmacy , University of Ljubljana 
      , Ljubljana , Slovenia.
FAU - Vujic, Zorica
AU  - Vujic Z
AD  - c Department of Pharmaceutical Chemistry, Faculty of Pharmacy , University of 
      Belgrade , Belgrade , Serbia.
FAU - Crevar-Sakac, Milkica
AU  - Crevar-Sakac M
AD  - c Department of Pharmaceutical Chemistry, Faculty of Pharmacy , University of 
      Belgrade , Belgrade , Serbia.
FAU - Sopic, Miron
AU  - Sopic M
AD  - a Department of Medical Biochemistry, Faculty of Pharmacy , University of 
      Belgrade , Belgrade , Serbia.
FAU - Munjas, Jelena
AU  - Munjas J
AD  - a Department of Medical Biochemistry, Faculty of Pharmacy , University of 
      Belgrade , Belgrade , Serbia.
FAU - Radenkovic, Miroslav
AU  - Radenkovic M
AD  - d Department of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of 
      Medicine , University of Belgrade , Belgrade , Serbia.
FAU - Jelic-Ivanovic, Zorana
AU  - Jelic-Ivanovic Z
AD  - a Department of Medical Biochemistry, Faculty of Pharmacy , University of 
      Belgrade , Belgrade , Serbia.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Atherosclerosis/blood/*diet therapy/etiology
MH  - *Cynara scolymus
MH  - DNA Damage/*drug effects/physiology
MH  - Diet, Atherogenic/*adverse effects
MH  - Male
MH  - Plant Extracts/isolation & purification/pharmacology/*therapeutic use
MH  - *Plant Leaves
MH  - Rats
MH  - Rats, Wistar
PMC - PMC6130604
OTO - NOTNLM
OT  - Oxidative damage
OT  - gene expression
OT  - haemeoxygenase-1
OT  - monocyte chemoattractant protein-1
EDAT- 2018/02/08 06:00
MHDA- 2018/08/31 06:00
PMCR- 2018/02/06
CRDT- 2018/02/08 06:00
PHST- 2018/02/08 06:00 [entrez]
PHST- 2018/02/08 06:00 [pubmed]
PHST- 2018/08/31 06:00 [medline]
PHST- 2018/02/06 00:00 [pmc-release]
AID - 1434549 [pii]
AID - 10.1080/13880209.2018.1434549 [doi]
PST - ppublish
SO  - Pharm Biol. 2018 Dec;56(1):138-144. doi: 10.1080/13880209.2018.1434549.