PMID- 29409956
OWN - NLM
STAT- MEDLINE
DCOM- 20180322
LR  - 20180322
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 496
IP  - 4
DP  - 2018 Feb 19
TI  - The interaction between calcineurin and alpha-synuclein is regulated by calcium and 
      calmodulin.
PG  - 1109-1114
LID - S0006-291X(18)30169-4 [pii]
LID - 10.1016/j.bbrc.2018.01.148 [doi]
AB  - Calcineurin (CN) is a protein phosphatase and widely distributed in eukaryotes, 
      with an extremely high level of expression in mammalian brain. Alpha-synuclein 
      (alpha-syn) is a small soluble protein expressed primarily at presynaptic terminals 
      in the central nervous system. In our present study, we explored the interactions 
      between CN and alpha-syn in vitro. Based on the data from microscale thermophoresis, 
      GST pull-down assays, and co-immunoprecipitation, we found that CN binds alpha-syn. 
      Furthermore, this interaction is mediated by calcium/calmodulin (Ca(2+)/CaM) 
      signaling. Additionally, thapsigargin (TG) triggered an increase in CN activity 
      and alpha-syn aggregation in HEK293 cells stably transfected with alpha-syn. Our previous 
      study in vivo suggest that overexpression of alpha-syn in transgenic mice 
      significantly promoted CN activity and subsequent nuclear translocation of 
      nuclear factor of activated T-cells (NFAT) in the midbrain dopaminergic (mDA) 
      neurons. These in vivo and in vitro studies have been complementary with each 
      other, representing the changes in the CN-dependent pathway affected by 
      overexpression of alpha-syn.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Shi, Xiaoyu
AU  - Shi X
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China; College of Life Sciences, Langfang Normal University, Hebei, 065000, 
      China.
FAU - Sun, Yue
AU  - Sun Y
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China.
FAU - Wang, Ping
AU  - Wang P
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China.
FAU - Gu, Lingling
AU  - Gu L
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China.
FAU - Wang, Lu
AU  - Wang L
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China.
FAU - Yang, Huan
AU  - Yang H
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China.
FAU - Wei, Qun
AU  - Wei Q
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China.
FAU - Li, Zhimei
AU  - Li Z
AD  - Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, 
      China National Clinical Research Center for Neurological Diseases, Beijing 
      100050, China. Electronic address: lizm1211@163.com.
FAU - Luo, Jing
AU  - Luo J
AD  - Gene Engineering and Biotechnology, Beijing Key Laboratory, College of Life 
      Sciences, Beijing Normal University, No.19, Xinjiekouwaidajie, Beijing 100875, 
      China. Electronic address: luojing@bnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180207
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Calmodulin)
RN  - 0 (alpha-Synuclein)
RN  - EC 3.1.3.16 (Calcineurin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcineurin/*metabolism
MH  - Calcium/*metabolism
MH  - Calmodulin/*metabolism
MH  - Gene Expression Regulation/*physiology
MH  - HEK293 Cells
MH  - Humans
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - Signal Transduction/*physiology
MH  - alpha-Synuclein/*metabolism
OTO - NOTNLM
OT  - Alpha-synuclein
OT  - Calcineurin
OT  - Co-immunoprecipitation
OT  - GST pull-down assays
OT  - Microscale thermophoresis
OT  - Thapsigargin
EDAT- 2018/02/08 06:00
MHDA- 2018/03/23 06:00
CRDT- 2018/02/08 06:00
PHST- 2018/01/16 00:00 [received]
PHST- 2018/01/24 00:00 [accepted]
PHST- 2018/02/08 06:00 [pubmed]
PHST- 2018/03/23 06:00 [medline]
PHST- 2018/02/08 06:00 [entrez]
AID - S0006-291X(18)30169-4 [pii]
AID - 10.1016/j.bbrc.2018.01.148 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Feb 19;496(4):1109-1114. doi: 
      10.1016/j.bbrc.2018.01.148. Epub 2018 Feb 7.