PMID- 29410658
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic 
      Water Masses.
PG  - 24
LID - 10.3389/fmicb.2018.00024 [doi]
LID - 24
AB  - One of the most abundant archaeal groups on Earth is the Thaumarchaeota. They are 
      recognized as major contributors to marine ammonia oxidation, a crucial step in 
      the biogeochemical cycling of nitrogen. Their universal success is attributed to 
      a high genomic flexibility and niche adaptability. Based on differences in the 
      gene coding for ammonia monooxygenase subunit A (amoA), two different ecotypes 
      with distinct distribution patterns in the water column have been identified. We 
      used high-throughput sequencing of 16S rRNA genes combined with archaeal amoA 
      functional gene clone libraries to investigate which environmental factors are 
      driving the distribution of Thaumarchaeota ecotypes in the Atlantic gateway to 
      the Arctic Ocean through an annual cycle in 2014. We observed the characteristic 
      vertical pattern of Thaumarchaeota abundance with high values in the mesopelagic 
      (>200 m) water throughout the entire year, but also in the epipelagic (<200 m) 
      water during the dark winter months (January, March and November). The 
      Thaumarchaeota community was dominated by three OTUs which on average comprised 
      76% +/- 11 and varied in relative abundance according to water mass characteristics 
      and not to depth or ammonium concentration, as suggested in previous studies. The 
      ratios of the abundance of the different OTU types were similar to that of the 
      functional amoA water cluster types. Together, this suggests a strong selection 
      of ecotypes within different water masses, supporting the general idea of water 
      mass characteristics as an important factor in defining microbial community 
      structure. If indeed, as suggested in this study, Thaumarchaeota population 
      dynamics are controlled by a set of factors, described here as water mass 
      characteristics and not just depth alone, then changes in water mass flow will 
      inevitably affect the distribution of the different ecotypes.
FAU - Muller, Oliver
AU  - Muller O
AD  - Department of Microbiology, University of Bergen, Bergen, Norway.
FAU - Wilson, Bryan
AU  - Wilson B
AD  - Department of Microbiology, University of Bergen, Bergen, Norway.
FAU - Paulsen, Maria L
AU  - Paulsen ML
AD  - Department of Microbiology, University of Bergen, Bergen, Norway.
FAU - Ruminska, Agnieszka
AU  - Ruminska A
AD  - Department of Microbiology, University of Bergen, Bergen, Norway.
FAU - Armo, Hilde R
AU  - Armo HR
AD  - Department of Microbiology, University of Bergen, Bergen, Norway.
FAU - Bratbak, Gunnar
AU  - Bratbak G
AD  - Department of Microbiology, University of Bergen, Bergen, Norway.
FAU - Ovreas, Lise
AU  - Ovreas L
AD  - Department of Microbiology, University of Bergen, Bergen, Norway.
AD  - University Center in Svalbard (UNIS), Longyearbyen, Norway.
LA  - eng
PT  - Journal Article
DEP - 20180123
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5787140
OTO - NOTNLM
OT  - 16S rRNA gene sequencing
OT  - Arctic Ocean
OT  - ammonia-oxidation
OT  - amoA
OT  - ecotype
OT  - thaumarchaeota
OT  - water mass
EDAT- 2018/02/08 06:00
MHDA- 2018/02/08 06:01
PMCR- 2018/01/23
CRDT- 2018/02/08 06:00
PHST- 2017/10/09 00:00 [received]
PHST- 2018/01/08 00:00 [accepted]
PHST- 2018/02/08 06:00 [entrez]
PHST- 2018/02/08 06:00 [pubmed]
PHST- 2018/02/08 06:01 [medline]
PHST- 2018/01/23 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.00024 [doi]
PST - epublish
SO  - Front Microbiol. 2018 Jan 23;9:24. doi: 10.3389/fmicb.2018.00024. eCollection 
      2018.