PMID- 29411538
OWN - NLM
STAT- MEDLINE
DCOM- 20191028
LR  - 20191028
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 22
IP  - 4
DP  - 2018 Apr
TI  - SPION-mediated miR-141 promotes the differentiation of HuAESCs into dopaminergic 
      neuron-like cells via suppressing lncRNA-HOTAIR.
PG  - 2299-2310
LID - 10.1111/jcmm.13512 [doi]
AB  - In this study, a bioinformatics analysis and luciferase reporter assay revealed 
      that microRNA-141 could silence the expression of lncRNA-HOTAIR by binding to 
      specific sites on lncRNA-HOTAIR. We used superparamagnetic iron oxide 
      nanoparticles (SPIONs) to mediate the high expression of microRNA-141 
      (SPIONs@miR-141) in human amniotic epithelial stem cells (HuAESCs), which was 
      followed by the induction of the differentiation of HuAESCs into dopaminergic 
      neuron-like cells (iDNLCs). qPCR, western blot, immunofluorescence staining and 
      HPLC all suggested that SPION-mediated overexpression of miR-141 could promote an 
      increased expression of brain-derived neurotrophic factor (BDNF), DAT and 5-TH in 
      HuAESC-derived iDNLCs. The RIP and ChIP assay also showed that overexpression of 
      miR-141 could significantly inhibit the recruitment and binding of lncRNA-HOTAIR 
      to EZH2 on BDNF gene promoter. cDNA microarray analysis revealed that the 
      expression levels of 190 genes were much higher in iDNLCs than in HuAESCs. 
      Finally, a protein interaction network analysis and identification showed that in 
      the iDNLC group with SPIONs@miR-141, factors that interact with BDNF, such as 
      FGF8, SHH, NTRK3 and CREB1, all showed significantly higher expression levels 
      compared with those in the SPIONs@miR-Mut. Therefore, this study confirmed that 
      the highly efficient expression of microRNA-141 mediated by SPIONs could improve 
      the efficiency of HuAESCs differentiation into dopaminergic neuron-like cells.
CI  - (c) 2018 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Liu, Te
AU  - Liu T
AUID- ORCID: 0000-0003-1514-9548
AD  - Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai 
      University of Traditional Chinese Medicine, Shanghai, China.
AD  - Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
FAU - Zhang, Hu
AU  - Zhang H
AD  - Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai 
      University of Traditional Chinese Medicine, Shanghai, China.
FAU - Zheng, Jiajia
AU  - Zheng J
AD  - Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai 
      University of Traditional Chinese Medicine, Shanghai, China.
FAU - Lin, Jiajia
AU  - Lin J
AD  - Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai 
      University of Traditional Chinese Medicine, Shanghai, China.
FAU - Huang, Yongyi
AU  - Huang Y
AD  - Shanghai Topbiox Co Ltd, Shanghai, China.
FAU - Chen, Jiulin
AU  - Chen J
AD  - Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai 
      University of Traditional Chinese Medicine, Shanghai, China.
FAU - Yu, Zhihua
AU  - Yu Z
AD  - Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai 
      University of Traditional Chinese Medicine, Shanghai, China.
FAU - Guo, Lihe
AU  - Guo L
AD  - Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological 
      Sciences, Chinese Academy of Sciences, Shanghai, China.
FAU - Pan, Weidong
AU  - Pan W
AD  - Department of Neurology, Shuguang Hospital affiliated to Shanghai University of 
      Traditional Chinese Medicine, Shanghai, China.
FAU - Xiong, Ying
AU  - Xiong Y
AD  - Department of Gynaecology and Obstetrics, Xinhua hospital affiliated to Shanghai 
      Jiaotong University School of Medicine, Shanghai, China.
FAU - Chen, Chuan
AU  - Chen C
AD  - Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai 
      University of Traditional Chinese Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180207
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Ferric Compounds)
RN  - 0 (MIRN141 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (long non-coding RNA HOTAIRM1, human)
RN  - 1K09F3G675 (ferric oxide)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Cell Differentiation/*genetics
MH  - Cell Line
MH  - Cell Proliferation/genetics
MH  - Computational Biology
MH  - Dopaminergic Neurons/*cytology/metabolism
MH  - Epithelial Cells/drug effects
MH  - Ferric Compounds/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Luciferases/chemistry
MH  - MicroRNAs/*genetics
MH  - Nanoparticles/administration & dosage
MH  - Promoter Regions, Genetic
PMC - PMC5867164
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - dopaminergic neuron-like cells (iDNLCs)
OT  - human amniotic epithelial stem cells (HuAESCs)
OT  - long non-coding RNA HOTAIR
OT  - magnetofection based on superparamagnetic iron oxide nanoparticles (SPIONs)
OT  - microRNA-141 (miR-141)
EDAT- 2018/02/08 06:00
MHDA- 2019/10/29 06:00
PMCR- 2018/04/01
CRDT- 2018/02/08 06:00
PHST- 2017/08/13 00:00 [received]
PHST- 2017/11/24 00:00 [accepted]
PHST- 2018/02/08 06:00 [pubmed]
PHST- 2019/10/29 06:00 [medline]
PHST- 2018/02/08 06:00 [entrez]
PHST- 2018/04/01 00:00 [pmc-release]
AID - JCMM13512 [pii]
AID - 10.1111/jcmm.13512 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2018 Apr;22(4):2299-2310. doi: 10.1111/jcmm.13512. Epub 2018 Feb 
      7.