PMID- 29412871 OWN - NLM STAT- MEDLINE DCOM- 20190603 LR - 20190603 IS - 1879-3592 (Electronic) IS - 1383-5718 (Linking) VI - 826 DP - 2018 Feb TI - In vivo antimutagenic and antiatherogenic effects of the (1 --> 3)(1 --> 6)-beta-d- glucan botryosphaeran. PG - 6-14 LID - S1383-5718(17)30050-5 [pii] LID - 10.1016/j.mrgentox.2017.12.008 [doi] AB - The antimutagenic effect of botryosphaeran, an exocellular (1 --> 3)(1 --> 6)-beta-d-glucan, from the ascomyceteous and plant-borne endophytic fungus, Botryosphaeria rhodina MAMB-05, was evaluated in young (6-8 weeks) and elderly (18 months) Swiss albino mice of both genders. The hypolipidemic, hypoglycemic and antiatherogenic potential was also evaluated in 18-month old male LDL receptor knockout (LDLr(-/-)) mice. Administration of botryosphaeran by gavage (doses: 7.5, 15, 30 mg/kg b.w./day) in a 30-day pretreatment protocol (young mice), or 15-day protocol (older mice), did not cause genotoxicity as assessed by the micronucleus test in peripheral blood (PB) and bone marrow cells (BMCs). Furthermore, there was no cytotoxic effect of this beta-d-glucan in the treatments. A lower frequency of micronuclei was observed in BMCs from young and old mice that received botryosphaeran, indicating its antimutagenic effect. Botryosphaeran (30 mg/kg b.w./day) promoted 102.22% (young) and 103.45% (elderly) reductions in cyclophosphamide-induced damage in male mice. Botryosphaeran also exerted chemoprotective effects in LDLr(-/-) and wild-type (C57BL/6) mice. Botryosphaeran treatment for 15 days at a dose of 30 mg/kg b.w./day improved the lipidic profile (reductions of 53.8-84.3%), and decreased aortic lipid deposition (32.8%) in the LDLr(-/-) atherosclerotic mice. The results indicate botryosphaeran has relevant biologic effects, making it a promising candidate for the development of new therapeutic agents. CI - Copyright (c) 2017 Elsevier B.V. All rights reserved. FAU - Silva-Sena, Geralda Gillian AU - Silva-Sena GG AD - Programa de Pos-Graduacao em Biotecnologia, Renorbio, Universidade Federal do Espirito Santo, CEP 29043-900, Vitoria, ES, Brazil; Departamento de EducacaoIntegrada em Saude, Centro de Ciencias da Saude, Universidade Federal do Espirito Santo, CEP 29043-900, Vitoria, ES, Brazil. Electronic address: ggsmais@gmail.com. FAU - Malini, Maressa AU - Malini M AD - Departamento de Biologia Geral, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, CEP 86057-970, Londrina, PR, Brazil. Electronic address: maressa.malini@gmail.com. FAU - Delarmelina, Juliana Macedo AU - Delarmelina JM AD - Programa de Pos-Graduacao em Biologia Vegetal, Centro de Ciencias Humanas e Naturais, Universidade Federal do Espirito Santo, CEP 29075-910, Vitoria, ES, Brazil. Electronic address: judelarmelina@yahoo.com.br. FAU - Dutra, Jean Carlos Vencioneck AU - Dutra JCV AD - Programa de Pos-Graduacao em Biologia Vegetal, Centro de Ciencias Humanas e Naturais, Universidade Federal do Espirito Santo, CEP 29075-910, Vitoria, ES, Brazil. Electronic address: jeanvencioneck@gmail.com. FAU - Gervasio, Suiany Vitorino AU - Gervasio SV AD - Departamento de Ciencias Biologicas, Centro de Ciencias Humanas e Naturais, Universidade Federal do Espirito Santo, CEP 29075-910, Vitoria, ES, Brazil. Electronic address: suianygervasio@gmail.com. FAU - Leal, Marcos Andre Soares AU - Leal MAS AD - Laboratorio de FisiologiaTranslacional, Universidade Federal do Espirito Santo, CEP 29043-900, Vitoria, ES, Brazil. Electronic address: marcos.emescam@gmail.com. FAU - Costa Pereira, Thiago de Melo AU - Costa Pereira TM AD - Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Vila Velha, CEP 29102-920, Vila Velha, ES, Brazil; Instituto de Educacao, Ciencia e Tecnologia (IFES), CEP 29106-010, Vila Velha, ES, Brazil. Electronic address: pereiratmc@gmail.com. FAU - Barbosa-Dekker, Aneli M AU - Barbosa-Dekker AM AD - Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario, P7 B 5E1, Canada; Departamento de Quimica, Centro de Ciencias Exatas-Universidade Estadual de Londrina, CEP 86057-970, Londrina, PR, Brazil. Electronic address: anelibarbosa@gmail.com. FAU - Dekker, Robert F H AU - Dekker RFH AD - Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario, P7 B 5E1, Canada; Programa de Pos-Graduacao em Engenharia Ambiental, Universidade Tecnologica Federal do Parana, Campus Londrina, CEP 86036-370, Londrina, PR, Brazil. Electronic address: xylanase@gmail.com. FAU - de Paula, Flavia AU - de Paula F AD - Programa de Pos-Graduacao em Biotecnologia, Renorbio, Universidade Federal do Espirito Santo, CEP 29043-900, Vitoria, ES, Brazil; Departamento de Ciencias Biologicas, Centro de Ciencias Humanas e Naturais, Universidade Federal do Espirito Santo, CEP 29075-910, Vitoria, ES, Brazil. Electronic address: flapvit@yahoo.com.br. FAU - Batitucci, Maria do Carmo Pimentel AU - Batitucci MDCP AD - Programa de Pos-Graduacao em Biologia Vegetal, Centro de Ciencias Humanas e Naturais, Universidade Federal do Espirito Santo, CEP 29075-910, Vitoria, ES, Brazil; Departamento de Ciencias Biologicas, Centro de Ciencias Humanas e Naturais, Universidade Federal do Espirito Santo, CEP 29075-910, Vitoria, ES, Brazil. Electronic address: docarmo_batitucci@yahoo.com.br. LA - eng PT - Journal Article DEP - 20171221 PL - Netherlands TA - Mutat Res Genet Toxicol Environ Mutagen JT - Mutation research. Genetic toxicology and environmental mutagenesis JID - 101632149 RN - 0 (Antimutagenic Agents) RN - 0 (Glucans) RN - 0 (Receptors, LDL) RN - 0 (botryosphaeran) RN - 8N3DW7272P (Cyclophosphamide) SB - IM MH - Animals MH - Antimutagenic Agents/*administration & dosage/pharmacology MH - Bone Marrow Cells/cytology/drug effects MH - Chemoprevention MH - Cyclophosphamide/*toxicity MH - Female MH - Gene Knockout Techniques MH - Glucans/*administration & dosage/pharmacology MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Micronucleus Tests MH - Receptors, LDL/*genetics MH - Saccharomycetales/*chemistry OTO - NOTNLM OT - (1 --> 3)(1 --> 6)-beta-d-glucan OT - Atheroprotection OT - Cytotoxicity OT - Genotoxicity OT - Glucose and lipidic profiles EDAT- 2018/02/08 06:00 MHDA- 2019/06/04 06:00 CRDT- 2018/02/08 06:00 PHST- 2017/02/22 00:00 [received] PHST- 2017/12/03 00:00 [revised] PHST- 2017/12/15 00:00 [accepted] PHST- 2018/02/08 06:00 [entrez] PHST- 2018/02/08 06:00 [pubmed] PHST- 2019/06/04 06:00 [medline] AID - S1383-5718(17)30050-5 [pii] AID - 10.1016/j.mrgentox.2017.12.008 [doi] PST - ppublish SO - Mutat Res Genet Toxicol Environ Mutagen. 2018 Feb;826:6-14. doi: 10.1016/j.mrgentox.2017.12.008. Epub 2017 Dec 21.