PMID- 29416543
OWN - NLM
STAT- MEDLINE
DCOM- 20190214
LR  - 20190215
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Neutralizing Antibodies Induced by Gene-Based Hydrodynamic Injection Have a 
      Therapeutic Effect in Lethal Influenza Infection.
PG  - 47
LID - 10.3389/fimmu.2018.00047 [doi]
LID - 47
AB  - The influenza virus causes annual epidemics and occasional pandemics and is thus 
      a major public health problem. Development of vaccines and antiviral drugs is 
      essential for controlling influenza virus infection. We previously demonstrated 
      the use of vectored immune-prophylaxis against influenza virus infection. We 
      generated a plasmid encoding neutralizing IgG monoclonal antibodies (mAbs) 
      against A/PR/8/34 influenza virus (IAV) hemagglutinin (HA). We then performed 
      electroporation of the plasmid encoding neutralizing mAbs (EP) in mice muscles 
      and succeeded in inducing the expression of neutralizing antibodies in mouse 
      serum. This therapy has a prophylactic effect against lethal IAV infection in 
      mice. In this study, we established a new method of passive immunotherapy after 
      IAV infection. We performed hydrodynamic injection of the plasmid encoding 
      neutralizing mAbs (HD) involving rapid injection of a large volume of plasmid-DNA 
      solution into mice via the tail vein. HD could induce neutralizing antibodies in 
      the serum and in several mucosal tissues more rapidly than in EP. We also showed 
      that a single HD completely protected the mice even after infection with a lethal 
      dose of IAV. We also established other isotypes of anti-HA antibody (IgA, IgM, 
      IgD, and IgE) and showed that like anti-HA IgG, anti-HA IgA was also effective at 
      combating upper respiratory tract IAV infection. Passive immunotherapy with HD 
      could thus provide a new therapeutic strategy targeting influenza virus 
      infection.
FAU - Yamazaki, Tatsuya
AU  - Yamazaki T
AD  - Department of Microbiology and Immunology, School of Medicine, Aichi Medical 
      University, Nagakute, Japan.
AD  - Department of Biological Science and Technology, Tokyo University of Science, 
      Katsushika, Japan.
FAU - Nagashima, Maria
AU  - Nagashima M
AD  - Department of Biological Science and Technology, Tokyo University of Science, 
      Katsushika, Japan.
FAU - Ninomiya, Daisuke
AU  - Ninomiya D
AD  - Department of Biological Science and Technology, Tokyo University of Science, 
      Katsushika, Japan.
FAU - Ainai, Akira
AU  - Ainai A
AD  - Department of Pathology, National Institute of Infectious Diseases, Shinjuku, 
      Japan.
FAU - Fujimoto, Akira
AU  - Fujimoto A
AD  - Department of Biological Science and Technology, Tokyo University of Science, 
      Katsushika, Japan.
FAU - Ichimonji, Isao
AU  - Ichimonji I
AD  - Department of Microbiology and Immunology, School of Medicine, Aichi Medical 
      University, Nagakute, Japan.
FAU - Takagi, Hidekazu
AU  - Takagi H
AD  - Department of Microbiology and Immunology, School of Medicine, Aichi Medical 
      University, Nagakute, Japan.
FAU - Morita, Naoko
AU  - Morita N
AD  - Department of Microbiology and Immunology, School of Medicine, Aichi Medical 
      University, Nagakute, Japan.
FAU - Murotani, Kenta
AU  - Murotani K
AD  - Division of Biostatistics, Clinical Research Center, School of Medicine, Aichi 
      Medical University, Nagakute, Japan.
FAU - Hasegawa, Hideki
AU  - Hasegawa H
AD  - Department of Pathology, National Institute of Infectious Diseases, Shinjuku, 
      Japan.
FAU - Chiba, Joe
AU  - Chiba J
AD  - Department of Biological Science and Technology, Tokyo University of Science, 
      Katsushika, Japan.
FAU - Akashi-Takamura, Sachiko
AU  - Akashi-Takamura S
AD  - Department of Microbiology and Immunology, School of Medicine, Aichi Medical 
      University, Nagakute, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180124
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Hemagglutinin Glycoproteins, Influenza Virus)
RN  - 0 (Influenza Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/genetics/*immunology
MH  - Antibodies, Viral/genetics/*immunology
MH  - Electroporation
MH  - Female
MH  - Hemagglutinin Glycoproteins, Influenza Virus/*immunology
MH  - Hydrodynamics
MH  - Immunization, Passive
MH  - Influenza Vaccines/*administration & dosage
MH  - Injections
MH  - Mice, Inbred BALB C
MH  - Orthomyxoviridae Infections/*therapy
MH  - Plasmids
MH  - Respiratory Mucosa/immunology/virology
PMC - PMC5787536
OTO - NOTNLM
OT  - gene therapy
OT  - hemagglutinin
OT  - hydrodynamics procedure
OT  - influenza
OT  - neutralizing antibody
OT  - passive immunotherapy
EDAT- 2018/02/09 06:00
MHDA- 2018/02/09 06:01
PMCR- 2018/01/01
CRDT- 2018/02/09 06:00
PHST- 2017/06/29 00:00 [received]
PHST- 2018/01/08 00:00 [accepted]
PHST- 2018/02/09 06:00 [entrez]
PHST- 2018/02/09 06:00 [pubmed]
PHST- 2018/02/09 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.00047 [doi]
PST - epublish
SO  - Front Immunol. 2018 Jan 24;9:47. doi: 10.3389/fimmu.2018.00047. eCollection 2018.