PMID- 29420632
OWN - NLM
STAT- MEDLINE
DCOM- 20180425
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 2
DP  - 2018
TI  - Implication of SPARC in the modulation of the extracellular matrix and 
      mitochondrial function in muscle cells.
PG  - e0192714
LID - 10.1371/journal.pone.0192714 [doi]
LID - e0192714
AB  - Secreted protein, acidic and rich in cysteine (SPARC) is differentially 
      associated with cell proliferation and extracellular matrix (ECM) assembly. We 
      show here the effect of exogenous SPARC inhibition/induction on ECM and 
      mitochondrial proteins expression and on the differentiation of C2C12 cells. The 
      cells were cultured in growth medium (GM) supplemented with different 
      experimental conditions. The differentiation of myoblasts was studied for 5 days, 
      the expressions of ECM and mitochondrial proteins were measured and the formation 
      of the myotubes was quantified after exogenous induction/inhibition of SPARC. The 
      results indicate that the addition of recombinant SPARC protein (rSPARC) in cell 
      culture medium increased the differentiation of C2C12 myoblasts and myogenin 
      expression during the myotube formation. However, the treatment with antibody 
      specific for SPARC (anti-SPARC) prevented the differentiation and decreased 
      myogenin expression. The induction of SPARC in the proliferating and 
      differentiating C2C12 cells increased collagen 1a1 protein expression, whereas 
      the inhibition decreased it. The effects on fibronectin protein expression were 
      opposite. Furthermore, the addition of rSPARC in C2C12 myoblast increased the 
      expression of mitochondrial proteins, ubiquinol-cytochrome c reductase core 
      protein II (UQCRC2) and succinate dehydrogenase iron-sulfur subunit (SDHB), 
      whereas the anti-SPARC decreased them. During the differentiation, only the 
      anti-SPARC had the effects on mitochondrial proteins, NADH dehydrogenase 
      ubiquinone 1 beta subcomplex subunit 8 (NADHB8), SDHB and cytochrome c oxidase 1 
      (MTCO1). Thus, SPARC plays a crucial role in the proliferation and 
      differentiation of C2C12 and may be involved in the link between the ECM 
      remodeling and mitochondrial function.
FAU - Melouane, Aicha
AU  - Melouane A
AD  - CREMI, CHU de Quebec Research Center, Quebec, Quebec, Canada.
AD  - Department of Molecular Medicine, Faculty of Medicine, Laval University, Quebec, 
      Quebec, Canada.
FAU - Carbonell, Antoine
AU  - Carbonell A
AD  - Unit of Human Genetics, CHU de Quebec Research Center, Quebec, Quebec, Canada.
AD  - Department of Medicine, Faculty of Medicine, Laval University, Quebec, Quebec, 
      Canada.
FAU - Yoshioka, Mayumi
AU  - Yoshioka M
AD  - CREMI, CHU de Quebec Research Center, Quebec, Quebec, Canada.
FAU - Puymirat, Jack
AU  - Puymirat J
AD  - Unit of Human Genetics, CHU de Quebec Research Center, Quebec, Quebec, Canada.
AD  - Department of Medicine, Faculty of Medicine, Laval University, Quebec, Quebec, 
      Canada.
FAU - St-Amand, Jonny
AU  - St-Amand J
AUID- ORCID: 0000-0002-2595-6127
AD  - CREMI, CHU de Quebec Research Center, Quebec, Quebec, Canada.
AD  - Department of Molecular Medicine, Faculty of Medicine, Laval University, Quebec, 
      Quebec, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180208
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Culture Media)
RN  - 0 (Osteonectin)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SPARC protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Line
MH  - Culture Media
MH  - Extracellular Matrix/*metabolism
MH  - Mice
MH  - Mitochondria, Muscle/*metabolism
MH  - Muscle, Skeletal/cytology/*metabolism
MH  - Myoblasts, Skeletal/cytology/*metabolism
MH  - Osteonectin/*metabolism
MH  - Oxidative Phosphorylation
MH  - Recombinant Proteins/metabolism
PMC - PMC5805355
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2018/02/09 06:00
MHDA- 2018/04/26 06:00
PMCR- 2018/02/08
CRDT- 2018/02/09 06:00
PHST- 2017/05/19 00:00 [received]
PHST- 2018/01/29 00:00 [accepted]
PHST- 2018/02/09 06:00 [entrez]
PHST- 2018/02/09 06:00 [pubmed]
PHST- 2018/04/26 06:00 [medline]
PHST- 2018/02/08 00:00 [pmc-release]
AID - PONE-D-17-19335 [pii]
AID - 10.1371/journal.pone.0192714 [doi]
PST - epublish
SO  - PLoS One. 2018 Feb 8;13(2):e0192714. doi: 10.1371/journal.pone.0192714. 
      eCollection 2018.