PMID- 29421306 OWN - NLM STAT- MEDLINE DCOM- 20180911 LR - 20180911 IS - 1872-7549 (Electronic) IS - 0166-4328 (Linking) VI - 346 DP - 2018 Jul 2 TI - Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats. PG - 1-10 LID - S0166-4328(17)31691-1 [pii] LID - 10.1016/j.bbr.2018.02.002 [doi] AB - In mammals, environmental factors including cold stress exert dramatic effects on adult health during late gestation, the cold stress response refers to an organism's response to cold. Indeed, cells and organs, including the hippocampus, are coordinated to respond to prevent hypothermia. The hippocampus act as an important brain structure that regulates the activity of the hypothalamic-pituitary-adrenal (HPA) axis, and suppress the stress reaction through feedback regulation of the HPA axis. To evaluate the response of the hippocampus during prenatal cold stress, we established a prenatal cold stress rat model. The molecular and signaling pathways responsible for the hippocampus cold exposure response were investigated. We assessed the glucocorticoid receptor, mineralocorticoid receptor, brain-derived neurotrophic factor (BDNF), RNA-binding motif protein 3 (RBM3), heat shock protein 70, protein expression, and extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and nuclear factor-kappa B pathways. Male and female offspring behavior were evaluated. Cold stress reduced the BDNF level in the maternal hippocampus in contrast to the increase in RBM3. BDNF has been shown to induce and RBM3 inhibits ERK phosphorylation. We measured p-ERK1/2 and showed low-level phosphorylation in the hippocampus after cold stress. Furthermore, we demonstrated that cold stress enhanced phosphorylation of P65 on Ser536, and led to apoptosis of the hippocampus in a caspase 3-independent manner. Behavioral tests were performed on pubescent male and female offspring, both of which showed evidence of reduced anxiety-like behavior. In summary, a more thorough understanding of these mechanisms may lead to maternal intervention that can reverse the damage of prenatal stressors or prevent the damage altogether and improve the physical quality of neonatal rats. CI - Copyright (c) 2018 Elsevier B.V. All rights reserved. FAU - Lian, Shuai AU - Lian S AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Wang, Di AU - Wang D AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Xu, Bin AU - Xu B AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Guo, Wenjin AU - Guo W AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Wang, Lipeng AU - Wang L AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Li, Wenjie AU - Li W AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Ji, Hong AU - Ji H AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Wang, Jianfa AU - Wang J AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Kong, Fanzhi AU - Kong F AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Zhen, Li AU - Zhen L AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Li, Shize AU - Li S AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Zhang, Liping AU - Zhang L AD - College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. FAU - Guo, Jingru AU - Guo J AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. Electronic address: guojingru0202@163.com. FAU - Yang, Huanmin AU - Yang H AD - College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. Electronic address: byndyhm@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180205 PL - Netherlands TA - Behav Brain Res JT - Behavioural brain research JID - 8004872 RN - 0 (RBM3 protein, rat) RN - 0 (RNA-Binding Proteins) RN - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) SB - IM MH - Animals MH - Anxiety/physiopathology MH - Apoptosis/physiology MH - *Behavior, Animal/physiology MH - Cold Temperature/*adverse effects MH - Extracellular Signal-Regulated MAP Kinases/genetics/metabolism MH - Female MH - Hippocampus/*pathology/physiopathology MH - MAP Kinase Signaling System/physiology MH - Male MH - Phosphorylation MH - Pregnancy MH - Pregnancy Complications/*pathology/physiopathology MH - *Prenatal Exposure Delayed Effects/physiopathology MH - RNA-Binding Proteins/metabolism MH - Random Allocation MH - Rats, Wistar MH - Specific Pathogen-Free Organisms MH - *Stress, Physiological/physiology OTO - NOTNLM OT - BDNF OT - ERK1/2 OT - Hippocampus OT - NF-kappaB OT - Offspring behavior OT - Prenatal cold stress EDAT- 2018/02/09 06:00 MHDA- 2018/09/12 06:00 CRDT- 2018/02/09 06:00 PHST- 2017/10/13 00:00 [received] PHST- 2018/02/02 00:00 [revised] PHST- 2018/02/02 00:00 [accepted] PHST- 2018/02/09 06:00 [pubmed] PHST- 2018/09/12 06:00 [medline] PHST- 2018/02/09 06:00 [entrez] AID - S0166-4328(17)31691-1 [pii] AID - 10.1016/j.bbr.2018.02.002 [doi] PST - ppublish SO - Behav Brain Res. 2018 Jul 2;346:1-10. doi: 10.1016/j.bbr.2018.02.002. Epub 2018 Feb 5.