PMID- 29421435
OWN - NLM
STAT- MEDLINE
DCOM- 20181120
LR  - 20181120
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 374
DP  - 2018 Mar 15
TI  - Long Non-coding RNA BC168687 is Involved in TRPV1-mediated Diabetic Neuropathic 
      Pain in Rats.
PG  - 214-222
LID - S0306-4522(18)30079-4 [pii]
LID - 10.1016/j.neuroscience.2018.01.049 [doi]
AB  - Long noncoding RNAs (lncRNAs) participate in a diverse range of molecular and 
      biological processes, and dysregulation of lncRNAs has been observed in the 
      pathogenesis of various human diseases. We observed alterations in mechanical 
      withdrawal thresholds (MWT) and thermal withdrawal latencies (TWL) in 
      streptozotocin (STZ)-induced diabetic rats treated with small interfering RNA 
      (siRNA) of lncRNA BC168687. We detected expression of transient receptor 
      potential vanilloid type 1 (TRPV1) in rat dorsal root ganglia (DRG) by a series 
      of molecular experiments. We determined relative levels of tumor necrosis factor 
      (TNF)-alpha and interleukin (IL)-1beta in rat serum by enzyme-linked immunosorbent assay 
      (ELISA). In addition, we examined extracellular regulated protein kinases (ERK) 
      and p38 mitogen-activated protein kinase (MAPK) signaling pathways by Western 
      blot (WB). We showed that the MWT and TWL of diabetic rats increased 
      significantly compared with control. Expression of TRPV1 receptors in DRG 
      substantially decreased. Relative levels of TNF-alpha and IL-1beta in the serum of 
      lncRNA BC168687 siRNA-treated rats were reduced. Phosphorylation (p)-ERK and 
      p-p38 signaling pathways in DRG were also decreased. Taken together, we concluded 
      lncRNA BC168687 siRNA may alleviate TRPV1-mediated diabetic neuropathic pain.
CI  - Copyright (c) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Liu, Chenglong
AU  - Liu C
AD  - Department of Physiology, Basic Medical College of Nanchang University, Nanchang 
      330006, PR China.
FAU - Li, Congcong
AU  - Li C
AD  - The Second Clinical Medical College of Nanchang University, Nanchang 330006, PR 
      China.
FAU - Deng, Zeyu
AU  - Deng Z
AD  - Department of Physiology, Basic Medical College of Nanchang University, Nanchang 
      330006, PR China.
FAU - Du, Errong
AU  - Du E
AD  - Department of Physiology, Basic Medical College of Nanchang University, Nanchang 
      330006, PR China.
FAU - Xu, Changshui
AU  - Xu C
AD  - Department of Physiology, Basic Medical College of Nanchang University, Nanchang 
      330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function 
      and Disease, Nanchang 330006, PR China. Electronic address: 
      xuchangshui@ncu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180205
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (BC168687 long noncoding RNA, rat)
RN  - 0 (IL1B protein, rat)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpv1 protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental/*metabolism/pathology/therapy
MH  - Diabetic Neuropathies/*metabolism/pathology/therapy
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Ganglia, Spinal/metabolism/pathology
MH  - Interleukin-1beta/blood
MH  - MAP Kinase Signaling System/physiology
MH  - Male
MH  - Neuralgia/*metabolism/pathology/therapy
MH  - Pain Threshold/physiology
MH  - RNA, Long Noncoding/antagonists & inhibitors/genetics/*metabolism
MH  - RNA, Small Interfering
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2X7/metabolism
MH  - TRPV Cation Channels/*metabolism
MH  - Tumor Necrosis Factor-alpha/blood
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - DRG
OT  - TRPV1
OT  - diabetic neuropathic pain
OT  - long non-coding RNAs
EDAT- 2018/02/09 06:00
MHDA- 2018/11/21 06:00
CRDT- 2018/02/09 06:00
PHST- 2017/11/02 00:00 [received]
PHST- 2018/01/22 00:00 [revised]
PHST- 2018/01/24 00:00 [accepted]
PHST- 2018/02/09 06:00 [pubmed]
PHST- 2018/11/21 06:00 [medline]
PHST- 2018/02/09 06:00 [entrez]
AID - S0306-4522(18)30079-4 [pii]
AID - 10.1016/j.neuroscience.2018.01.049 [doi]
PST - ppublish
SO  - Neuroscience. 2018 Mar 15;374:214-222. doi: 10.1016/j.neuroscience.2018.01.049. 
      Epub 2018 Feb 5.