PMID- 29422971
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 1
DP  - 2018 Jan
TI  - Novel and simple method of double-detection using fluorescence in situ 
      hybridization and fluorescence immunostaining of formalin-fixed paraffin-embedded 
      tissue sections.
PG  - 1084-1088
LID - 10.3892/ol.2017.7413 [doi]
AB  - The importance of fluorescence in situ hybridization (FISH) for pathological 
      diagnosis has been increasing. However, the procedures utilized for a 
      conventional FISH method with formalin-fixed paraffin-embedded tissue sections 
      are complicated and it is difficult to perform as a routine laboratory test. In 
      addition, there are difficulties with differentiation of targeted cells in 
      observations with a fluorescence microscope. The present study reported a novel 
      method that utilizes FISH in combination with fluorescence immunostaining as a 
      simple double-detection technique that addresses these problems. Using this novel 
      method, various genetic aberrations, as well as protein overexpression were 
      easily visualized in isologous sections. In particular, FISH signals with our 
      method clearly identify target cells in samples with poor differentiation between 
      tumor cells coexisting with normal cells. It is proposed that this simple 
      technique is widely applicable as a routine laboratory test and future 
      developments are expected.
FAU - Ikeda, Satoshi
AU  - Ikeda S
AD  - Department of Pathology, Tsuchiura Kyodo General Hospital, Tsuchiura, Ibaraki 
      300-0028, Japan.
LA  - eng
PT  - Journal Article
DEP - 20171115
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5772969
OTO - NOTNLM
OT  - double-detection
OT  - fluorescence immunohistochemistry
OT  - fluorescence in situ hybridization
OT  - human epidermal growth factor receptor 2
EDAT- 2018/02/10 06:00
MHDA- 2018/02/10 06:01
PMCR- 2017/11/15
CRDT- 2018/02/10 06:00
PHST- 2016/09/19 00:00 [received]
PHST- 2017/09/28 00:00 [accepted]
PHST- 2018/02/10 06:00 [entrez]
PHST- 2018/02/10 06:00 [pubmed]
PHST- 2018/02/10 06:01 [medline]
PHST- 2017/11/15 00:00 [pmc-release]
AID - OL-0-0-7413 [pii]
AID - 10.3892/ol.2017.7413 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Jan;15(1):1084-1088. doi: 10.3892/ol.2017.7413. Epub 2017 Nov 
      15.