PMID- 29423008
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 10
IP  - 1
DP  - 2018
TI  - Generation of an acute retinal photoreceptor degeneration model in rabbits.
PG  - 235-245
AB  - PURPOSE: To assess the appropriate dose of sodium nitroprusside for establishing 
      acute retinal photoreceptor degeneration models in rabbits. METHODS: Sodium 
      nitroprusside (SNP) was delivered intravitreously. Sixteen New Zealand White 
      rabbits are divided into four groups randomly: 0.1 mM, 0.25 mM, 0.5 mM SNP 
      intravitreal injection group (experimental groups), and normal saline 
      intravitreal injection group (control group). Assessments included weight, 
      anterior segment photography, fundus photography, Hematoxylin-eosin staining, 
      immunofluorescence, multi-focal electroretinogram (mfERG) and pupillary direct 
      light reflex were performed at baseline and day 28 after injection. The spectral 
      domain optical coherence tomography (SD-OCT) and full field electroretinogram 
      (fERG) were performed at baseline and day 1, 3, 7, 14, 21 and 28 after injection. 
      RESULTS: No complications and no significant different in weight were found among 
      all groups. No obvious change was found by slit lamp and fundus photography after 
      injection in all groups. In SD-OCT exams, a time-dependent and dose-dependent 
      injury of photoreceptor was found in SNP injection groups (P<0.05). The thickness 
      of inner nuclear and plexiform layer was significantly decreased in 0.5 mM group. 
      HE staining and immunofluorescence present the photoreceptor damage at the 
      posterior pole (0.1, 0.25, 0.5 mM groups) and periphery (0.5 mM group). fERG and 
      mfERG showed significant dose-dependent responses depression in SNP injection 
      groups (P<0.05). The pupillary direct light reflex in SNP groups declined 
      significantly at day 28(th) than pre-injection (P<0.05). CONCLUSIONS: Sodium 
      nitroprusside of 0.1 mM and 0.25 mM can lead to monolayer photoreceptor 
      degeneration at posterior pole in rabbits and the lesion is stable at 1 month 
      after SNP injection.
FAU - Li, Kang
AU  - Li K
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      UniversityGuangzhou 510060, Guangdong, China.
FAU - Liu, Shengxu
AU  - Liu S
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      UniversityGuangzhou 510060, Guangdong, China.
FAU - Zhong, Xiufeng
AU  - Zhong X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      UniversityGuangzhou 510060, Guangdong, China.
FAU - Ge, Jian
AU  - Ge J
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      UniversityGuangzhou 510060, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20180115
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5801361
OTO - NOTNLM
OT  - Animal model
OT  - photoreceptor degeneration
OT  - retinitis pigmentosa
OT  - sodium nitroprusside
COIS- None.
EDAT- 2018/02/10 06:00
MHDA- 2018/02/10 06:01
PMCR- 2018/01/15
CRDT- 2018/02/10 06:00
PHST- 2017/10/24 00:00 [received]
PHST- 2017/12/28 00:00 [accepted]
PHST- 2018/02/10 06:00 [entrez]
PHST- 2018/02/10 06:00 [pubmed]
PHST- 2018/02/10 06:01 [medline]
PHST- 2018/01/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2018 Jan 15;10(1):235-245. eCollection 2018.