PMID- 29424576
OWN - NLM
STAT- MEDLINE
DCOM- 20180828
LR  - 20180828
IS  - 1521-0758 (Electronic)
IS  - 0191-3123 (Linking)
VI  - 42
IP  - 2
DP  - 2018 Mar-Apr
TI  - Immunohistochemical and electron microscopic morphometric image analysis of 
      hepatocellular carcinoma in association of HCV infection.
PG  - 97-107
LID - 10.1080/01913123.2017.1422065 [doi]
AB  - Early detection of hepatocellular carcinoma (HCC) is crucial for successful 
      therapy. The present work examined the value of ultrastructural morphometric 
      image analysis of hepatocyte nuclei in patients with chronic hepatitis C virus 
      (HCV) versus HCC cases with chronic HCV and the corresponding surgical tumor-free 
      safe margins (TFMs), to highlight any early predictive signs of neoplastic 
      cellular transformation. This work also performed an immunohistochemical 
      assessment of cytokeratin 19 (CK19) and Ki-67-positive cells to visualize any 
      associated proliferative activity in the examined groups. The results showed 
      significant decrease in the hepatocyte nuclear surface areas in the HCC and TFMs 
      versus those in the HCV cases. The hepatocyte nucleolar surface area was 
      significantly increased in the HCC cases versus that in the HCV cases. This 
      increase was associated with a significant increase in Ki-67-positive cells in 
      the HCC cases compared to those in the other groups. Conversely, the mean number 
      of CK 19-positive cells was significantly reduced in the HCC cases compared to 
      the cell numbers in TFMs and HCV cases with severe hepatic fibrosis. Liver 
      progenitor cells (LPCs) were discerned in the reactive ductules and 
      canaliculo-ductular junctions that characterized TFMs. LPCs were sporadically 
      distributed in the liver lobules and reactive bile ductules in the HCC samples. 
      In conclusion, CK 19 represents an important marker for distinguishing between 
      dysplastic and malignant liver nodules. Electron microscopic morphometric image 
      analysis may be considered as adjunct factor for assessing hepatocyte malignant 
      transformation. Wider scale studies are needed to authenticate these results.
FAU - Hassan, Sarah
AU  - Hassan S
AD  - a Electron Microscopy Research Department (Pathology) , Theodor Bilharz Research 
      Institute , Cairo , Egypt.
FAU - Mansy, Soheir S
AU  - Mansy SS
AD  - a Electron Microscopy Research Department (Pathology) , Theodor Bilharz Research 
      Institute , Cairo , Egypt.
FAU - Tabak, Sahar A
AU  - Tabak SA
AD  - b Pathology Department, Faculty of Medicine , Cairo University , Egypt.
FAU - AbdelFattah, Ahmed S
AU  - AbdelFattah AS
AD  - c Hepatogastroenterology Department , Theodor Bilharz Research Institute , Cairo 
      , Egypt.
FAU - Abdel-Aziz, Ahmed M
AU  - Abdel-Aziz AM
AD  - b Pathology Department, Faculty of Medicine , Cairo University , Egypt.
FAU - Hamam, Olfat
AU  - Hamam O
AD  - d Pathology Department , Theodor Bilharz Research Institute , Cairo , Egypt.
FAU - Seleem, Mohammed I
AU  - Seleem MI
AD  - e Hepatobiliary Surgery and Liver Transplantation , National Hepatology and 
      Tropical Medicine Research Institute , Cairo , Egypt.
FAU - Abdelaal, Amr
AU  - Abdelaal A
AD  - f Surgery Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt.
LA  - eng
PT  - Journal Article
DEP - 20180209
PL  - England
TA  - Ultrastruct Pathol
JT  - Ultrastructural pathology
JID - 8002867
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Keratin-19)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Hepatocellular/*pathology/ultrastructure/virology
MH  - Cell Transformation, Neoplastic/*pathology/ultrastructure
MH  - Hepatitis C, Chronic/complications/pathology
MH  - Humans
MH  - Image Interpretation, Computer-Assisted
MH  - Immunohistochemistry
MH  - Keratin-19/analysis/biosynthesis
MH  - Liver Neoplasms/*pathology/ultrastructure/virology
MH  - Microscopy, Electron, Transmission
OTO - NOTNLM
OT  - HCC
OT  - Ki-67
OT  - chronic HCV
OT  - cytokeratin 19
OT  - liver progenitor cells
OT  - ultrastructural morphometric image analysis
EDAT- 2018/02/10 06:00
MHDA- 2018/08/29 06:00
CRDT- 2018/02/10 06:00
PHST- 2018/02/10 06:00 [pubmed]
PHST- 2018/08/29 06:00 [medline]
PHST- 2018/02/10 06:00 [entrez]
AID - 10.1080/01913123.2017.1422065 [doi]
PST - ppublish
SO  - Ultrastruct Pathol. 2018 Mar-Apr;42(2):97-107. doi: 
      10.1080/01913123.2017.1422065. Epub 2018 Feb 9.