PMID- 29425254
OWN - NLM
STAT- MEDLINE
DCOM- 20180412
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 2
DP  - 2018
TI  - Conservation and lineage-specific rearrangements in the GOBP/PBP gene complex of 
      distantly related ditrysian Lepidoptera.
PG  - e0192762
LID - 10.1371/journal.pone.0192762 [doi]
LID - e0192762
AB  - General odorant binding proteins (GOBPs) and pheromone binding proteins (PBPs) 
      form a monophyletic subfamily of insect odorant binding proteins (OBPs) specific 
      for Lepidoptera, butterflies and moths. The GOBP/PBP genes include six subgroups 
      (GOBP1-2, PBP-A-D) previously reported to form a complex arrayed in a conserved 
      order in representative moths (superfamily Bombycoidea) and butterflies 
      (Nymphalidae). Although our knowledge of lepidopteran genomes has increased 
      greatly recently, the structure of the GOBP/PBP complex has been studied only for 
      species that represent limited lineages of the highly diverged Ditrysia. To 
      understand the evolution of this functionally important gene complex, we 
      determined 69-149 kb genomic sequences that include GOBP2 and five PBP genes in 
      three Ostrinia moths (Pyraloidea), O. nubilalis, O. furnacalis, and O. 
      latipennis, using bacterial artificial chromosome (BAC) and fosmid clones. The 
      structure of the GOBP2/PBP gene cluster was well conserved despite the different 
      sex pheromone composition utilized by the three moths. Five expressed PBP genes 
      in Ostrinia moths were the result of two duplications of PBP-A genes. 
      Surprisingly, an allele containing a fusion gene between tandemly arrayed PBP-A 
      genes was observed in O. nubilalis. We also revealed duplication and 
      intra-chromosomal translocation of the GOBP1 gene in P. xylostella by 
      fluorescence in situ hybridization (FISH) analysis. Additionally, we compared the 
      structure of the GOBP/PBP gene complex of seventeen species covering six 
      superfamilies and twelve families of the lepidopteran clade, Ditrysia, and found 
      the gene order was basically conserved despite the frequent occurrence of 
      lineage-specific gains, losses, inversions and translocations of these genes, 
      compared with their neighboring genes. Our findings support the hypothesis that 
      the structure of the GOBP/PBP gene complex was already established in the common 
      ancestor of Ditrysia.
FAU - Yasukochi, Yuji
AU  - Yasukochi Y
AUID- ORCID: 0000-0003-1541-4057
AD  - Institute of Agrobiological Sciences, NARO, Tsukuba, Ibaraki, Japan.
FAU - Yang, Bin
AU  - Yang B
AD  - Department of Agricultural and Environmental Biology, Graduate School of 
      Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
FAU - Fujimoto, Toshiaki
AU  - Fujimoto T
AD  - Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
FAU - Sahara, Ken
AU  - Sahara K
AD  - Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
FAU - Matsuo, Takashi
AU  - Matsuo T
AD  - Department of Agricultural and Environmental Biology, Graduate School of 
      Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
FAU - Ishikawa, Yukio
AU  - Ishikawa Y
AD  - Department of Agricultural and Environmental Biology, Graduate School of 
      Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180209
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Carrier Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Receptors, Odorant)
RN  - 0 (odorant-binding protein)
RN  - 0 (pheromone binding protein, insect)
SB  - IM
EIN - PLoS One. 2018 May 10;13(5):e0197528. PMID: 29746577
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Chromosomes, Artificial, Bacterial
MH  - In Situ Hybridization, Fluorescence
MH  - Insect Proteins/*genetics
MH  - Lepidoptera/*genetics
MH  - Multigene Family
MH  - Phylogeny
MH  - Receptors, Odorant/*genetics
MH  - Transcription, Genetic
MH  - Translocation, Genetic
PMC - PMC5806886
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2018/02/10 06:00
MHDA- 2018/04/13 06:00
PMCR- 2018/02/09
CRDT- 2018/02/10 06:00
PHST- 2017/11/14 00:00 [received]
PHST- 2018/01/30 00:00 [accepted]
PHST- 2018/02/10 06:00 [entrez]
PHST- 2018/02/10 06:00 [pubmed]
PHST- 2018/04/13 06:00 [medline]
PHST- 2018/02/09 00:00 [pmc-release]
AID - PONE-D-17-40394 [pii]
AID - 10.1371/journal.pone.0192762 [doi]
PST - epublish
SO  - PLoS One. 2018 Feb 9;13(2):e0192762. doi: 10.1371/journal.pone.0192762. 
      eCollection 2018.