PMID- 29427035
OWN - NLM
STAT- MEDLINE
DCOM- 20181220
LR  - 20181220
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 185
IP  - 2
DP  - 2018 Oct
TI  - Probiotics as a Complementary Therapy in the Model of Cadmium Chloride Toxicity: 
      Crosstalk of beta-Catenin, BDNF, and StAR Signaling Pathways.
PG  - 404-413
LID - 10.1007/s12011-018-1261-x [doi]
AB  - Cadmium chloride (CdCl(2)) is a ubiquitous environmental toxicant that causes a 
      variety of disturbances in biological systems, including brain dysfunction and 
      testicular tissue degeneration. On the other hand, it is supposed that beneficial 
      properties of probiotic bacteria (Lactobacillus and Acidobacillus) are related to 
      their capacity to adhere or bind different targets, thus leading to improved 
      intestinal microbial balance and other benefits to the host. Bearing 
      aforementioned in mind, the present study was undertaken to investigate the 
      protective effect of probiotic supplementation against cadmium chloride-induced 
      brain and testis toxicity in mice model. Animals received Lactobacillus and 
      Acidobacillus either alone or added to folic acid for 1 week before CdCl2 
      intoxication in a dose of 20 mg/kg BW followed by probiotics (5 x 10(9)) and/or 
      folic acid (12 mg/kg) treatment for 3 weeks. The levels of malondialdehyde (MDA), 
      butyrl choline esterase (BCHE), reduced glutathione (GSH), and total superoxide 
      dismutase (SOD) activities were investigated. Finally, cadmium neurotoxicity was 
      determined by estimating the gene expression of beta-catenin and brain-derived 
      neurotrophic factor (BDNF), as well as estimating the alterations in testicular 
      function by determining acid phosphatase level in addition to steroidogenic acute 
      regulatory protein (StAR) and 17-hydroxy steroid dehydrogenase (17-beta HSD) gene 
      expression. Based on our results, we can conclude that exposure of mice to 
      cadmium chloride resulted in a significant elevation in MDA, BCHE levels 
      accompanied with a significant reduction in GSH and SOD activities compared to 
      the control value. CdCl(2) also downregulated the gene expression of beta-catenin 
      and BDNF, as well as acid phosphatase level, in addition to StAR and 17-beta HSD 
      gene expression. These deviated parameters were significantly modulated in the 
      co-treated animals with probiotics compared with the cadmium-treated group. In 
      conclusion, Lactobacillus and folic acid in a mixture with cadmium acted 
      beneficially to an organism, increasing the cadmium excretion in feces, and 
      consequently increasing beta-catenin and BDNF in brain tissue and StAR and 17-beta HSD 
      in testis and improving their functions. Histoarchitecture analysis confirmed 
      these results.
FAU - Kadry, Mai O
AU  - Kadry MO
AD  - Biochemistry, Therapeutic Chemistry Department, National Research Centre, Cairo, 
      Egypt. maiosman666@yahoo.com.
FAU - Megeed, Rehab Abdel
AU  - Megeed RA
AD  - Molecular Biology, Therapeutic Chemistry Department, National Research Centre, 
      Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20180209
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Phosphoproteins)
RN  - 0 (beta Catenin)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 935E97BOY8 (Folic Acid)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Animals
MH  - Brain Injuries/chemically induced/pathology
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cadmium Chloride/administration & dosage/*antagonists & inhibitors/*toxicity
MH  - Complementary Therapies
MH  - Disease Models, Animal
MH  - Folic Acid/administration & dosage/*pharmacology
MH  - Lactobacillus/*isolation & purification
MH  - Male
MH  - Mice
MH  - Phosphoproteins/genetics/*metabolism
MH  - *Probiotics
MH  - Signal Transduction/drug effects
MH  - beta Catenin/genetics/*metabolism
OTO - NOTNLM
OT  - 17- HSD
OT  - BDNF
OT  - CdCl2
OT  - StAR
OT  - beta-catenin
EDAT- 2018/02/11 06:00
MHDA- 2018/12/21 06:00
CRDT- 2018/02/11 06:00
PHST- 2017/12/03 00:00 [received]
PHST- 2018/02/01 00:00 [accepted]
PHST- 2018/02/11 06:00 [pubmed]
PHST- 2018/12/21 06:00 [medline]
PHST- 2018/02/11 06:00 [entrez]
AID - 10.1007/s12011-018-1261-x [pii]
AID - 10.1007/s12011-018-1261-x [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2018 Oct;185(2):404-413. doi: 10.1007/s12011-018-1261-x. 
      Epub 2018 Feb 9.