PMID- 29428398
OWN - NLM
STAT- MEDLINE
DCOM- 20180926
LR  - 20180926
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 255
DP  - 2018 May
TI  - Real-time quantitative isothermal detection of Ostreid herpesvirus-1 DNA in 
      Scapharca subcrenata using recombinase polymerase amplification.
PG  - 71-75
LID - S0166-0934(17)30757-7 [pii]
LID - 10.1016/j.jviromet.2018.02.007 [doi]
AB  - Ostreid herpesvirus-1 (OsHV-1) is a well-known pathogen associated with high 
      mortality rates in hatchery-reared larvae and juveniles of different bivalve 
      species worldwide. Early, rapid and accurate diagnosis plays a fundamental role 
      in disease prevention and control in aquaculture. Recombinase polymerase 
      amplification (RPA) is a novel isothermal amplification method, which can amplify 
      detectable amount of DNA at 37  degrees C-39  degrees C within 20 min. In the present study, two 
      sets of specific primers and probes were designed for the real-time quantitative 
      RPA (qRPA) detection of OsHV-1 DNA. The sensitivity and specificity of detection 
      were evaluated by comparison with quantitative polymerase chain reaction (qPCR). 
      The detection limit for qRPA assays was shown to be 5 copies DNA/reaction for the 
      primer set ORF95, which was lower than the 100 copies required for the qPCR test. 
      The optimal reaction temperature and time were 37  degrees C for 20 min, making this 
      approach faster than qPCR. This is the first study to apply qPCR and qRPA methods 
      to detect OsHV-1 in Scapharca subcrenata. The percentage of viral load sample 
      detected by the two methods was 22% and the correlation of the two virus 
      quantitative results was 0.8. Therefore, qRPA assays is sensitive, fast, and 
      high-temperature independent relative to qPCR and is suitable for critical 
      clinical diagnostics use and rapid field analysis in resource-limited settings.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Gao, Fang
AU  - Gao F
AD  - Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, 510300, China; Shanghai Ocean University, Shanghai, 201306, China. 
      Electronic address: tianchengyinuo@163.com.
FAU - Jiang, Jing-Zhe
AU  - Jiang JZ
AD  - Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, 510300, China. Electronic address: jingzhejiang@gmail.com.
FAU - Wang, Jiang-Yong
AU  - Wang JY
AD  - Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, 510300, China. Electronic address: wjy104@163.com.
FAU - Wei, Hong-Ying
AU  - Wei HY
AD  - Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, 510300, China; Shanghai Ocean University, Shanghai, 201306, China. 
      Electronic address: hongyingwei0104@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180208
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - DNA Viruses/*genetics
MH  - Herpesviridae Infections/*diagnosis/*virology
MH  - *Nucleic Acid Amplification Techniques
MH  - Polymerase Chain Reaction
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproducibility of Results
MH  - Scapharca/*virology
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Aquaculture
OT  - Clinical diagnostics
OT  - Ostreid herpesvirus-1
OT  - Scapharca subcrenata
OT  - qRPA
EDAT- 2018/02/13 06:00
MHDA- 2018/09/27 06:00
CRDT- 2018/02/12 06:00
PHST- 2017/12/06 00:00 [received]
PHST- 2018/02/06 00:00 [revised]
PHST- 2018/02/07 00:00 [accepted]
PHST- 2018/02/13 06:00 [pubmed]
PHST- 2018/09/27 06:00 [medline]
PHST- 2018/02/12 06:00 [entrez]
AID - S0166-0934(17)30757-7 [pii]
AID - 10.1016/j.jviromet.2018.02.007 [doi]
PST - ppublish
SO  - J Virol Methods. 2018 May;255:71-75. doi: 10.1016/j.jviromet.2018.02.007. Epub 
      2018 Feb 8.