PMID- 29429182
OWN - NLM
STAT- MEDLINE
DCOM- 20180307
LR  - 20181202
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 53
IP  - 2
DP  - 2018 Feb 7
TI  - [The effect of miR-497 on laryngeal squamous cell carcinoma invasion through 
      modulating PlexinA4].
PG  - 124-130
LID - 10.3760/cma.j.issn.1673-0860.2018.02.008 [doi]
AB  - Objective: To study the roles of miR-497 and PlexinA4 in the progression of 
      laryngeal squamous cell carcinoma. Methods: The expressions of miR-497 and 
      PlexinA4 in fresh tumor specimens and adjacent normal mucosa tissues as well as 
      in cell lines of laryngeal squamous cell carcinoma (LSCC) were detected with 
      qRT-PCR and immunohistochemistry. The association of miR-497 and PlexinA4 
      expressions with clinicopathologic factors and their prognostic values in LSCC 
      were evaluated PlexinA4 siRNA and pcDNA3.1 (+ )/PlexinA4 plasmid were transfected 
      into the LSCC and measured by Transwell to evaluate their effect on the invasion 
      of LSCC. Results: miR-497 was low expression in LSCC, which related to 
      pathological differentiation, while PlexinA4 mRNA was high expression in LSCC. 
      Kaplan-Meier method showed that the prognosis of patients with high miR-497 
      expression was better than that of patients with low miR-497 expression 
      (chi(2)=10.342, P=0.001); . Cox regression analysis showed that miR-497 was an 
      independent prognostic factor for LSCC. The double luciferase reporter gene 
      showed that the variation of the fluorescence activity of wild type PlexinA4 was 
      significantly different from that of the control group (P<0.01). In Hep-2 and 
      TU212 cell line, the number of cells with PlexinA4 siRNA passing through the 
      compartments was 70.00+/-10.85 and 85.00+/-6.45, significantly higher than control (F 
      values were 30.251 and 23.936, both P<0.05), the number of cells with pcDNA3.1 (+ 
      ) /PlexinA4 was 170.56+/-11.95 and 142.00+/-10.43, also significantly less than 
      control (F values were 35.104 and 29.643, both P<0.05). Conclusion: The 
      expression of miR-497 in LSCC is decreased, indicating poor prognosis, which is 
      as an independent risk factor for prognosis of LSCC. miR-497 may modulate LSCC 
      invasion through PlexinA4.
FAU - Niu, J T
AU  - Niu JT
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital, Tianjin 
      Medical University, Tianjin 300221, China.
FAU - Liu, S G
AU  - Liu SG
AD  - Graduate School, Tianjin Medical University, Tianjin 300070, China.
FAU - Huang, Y W
AU  - Huang YW
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital, Tianjin 
      Medical University, Tianjin 300221, China.
FAU - Li, C
AU  - Li C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital, Tianjin 
      Medical University, Tianjin 300221, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
RN  - 0 (MIRN497 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Plxna4 protein, human)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Kaplan-Meier Estimate
MH  - Laryngeal Mucosa/metabolism
MH  - Laryngeal Neoplasms/*metabolism/mortality/pathology
MH  - MicroRNAs/*metabolism
MH  - Prognosis
MH  - RNA, Small Interfering
MH  - Receptors, Cell Surface/*metabolism
MH  - Transfection
OTO - NOTNLM
OT  - Carcinoma, squamous cell
OT  - Invasion
OT  - Laryngeal neoplasms
EDAT- 2018/02/13 06:00
MHDA- 2018/03/08 06:00
CRDT- 2018/02/13 06:00
PHST- 2018/02/13 06:00 [entrez]
PHST- 2018/02/13 06:00 [pubmed]
PHST- 2018/03/08 06:00 [medline]
AID - 10.3760/cma.j.issn.1673-0860.2018.02.008 [doi]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Feb 7;53(2):124-130. doi: 
      10.3760/cma.j.issn.1673-0860.2018.02.008.