PMID- 2943257 OWN - NLM STAT- MEDLINE DCOM- 19860916 LR - 20190903 IS - 0004-945X (Print) IS - 0004-945X (Linking) VI - 64 ( Pt 2) DP - 1986 Apr TI - Isoenzyme studies of Giardia lamblia isolated from symptomatic cases. PG - 119-26 AB - Strains of Giardia lamblia were isolated from symptomatic cases of giardiasis and axenized in the laboratory. Electrophoretic mobility patterns of four enzymes, viz., EC 5.3.1.9 glucose phosphate isomerase (GPI); EC 1.1.1,4.0.L-malate; NADP+ Oxidoreductase (Oxaloacetate decarboxylating) (ME); EC 2.7.5.1 phosphoglucomutase (PGM); and EC 2.7.1.1 hexokinase (HK) of the lysates prepared from these isolates were studied using starch-gel. Based on differences in mobility patterns of PGM and HK, the four strains studied could be grouped into three different isoenzyme types (Zymodemes). ME mobility was identical in all the four strains. Some relative difference was seen in the mobility of GPI, though the pattern of mobility was similar in all the strains. FAU - Baveja, U K AU - Baveja UK FAU - Jyoti, A S AU - Jyoti AS FAU - Kaur, M AU - Kaur M FAU - Agarwal, D S AU - Agarwal DS FAU - Anand, B S AU - Anand BS FAU - Nanda, R AU - Nanda R LA - eng PT - Case Reports PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Australia TA - Aust J Exp Biol Med Sci JT - The Australian journal of experimental biology and medical science JID - 0416662 RN - 0 (Isoenzymes) RN - EC 1.1.1.37 (Malate Dehydrogenase) RN - EC 1.1.1.40 (malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)) RN - EC 2.7.1.1 (Hexokinase) RN - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase) RN - EC 5.4.2.2 (Phosphoglucomutase) SB - IM MH - Adolescent MH - Animals MH - Child MH - Duodenum/parasitology MH - Electrophoresis, Starch Gel MH - Female MH - Giardia/classification/*enzymology/isolation & purification MH - Giardiasis/*parasitology MH - Glucose-6-Phosphate Isomerase/metabolism MH - Hexokinase/metabolism MH - Humans MH - Isoenzymes/*metabolism MH - Malate Dehydrogenase/metabolism MH - Male MH - Middle Aged MH - Phosphoglucomutase/metabolism EDAT- 1986/04/01 00:00 MHDA- 1986/04/01 00:01 CRDT- 1986/04/01 00:00 PHST- 1986/04/01 00:00 [pubmed] PHST- 1986/04/01 00:01 [medline] PHST- 1986/04/01 00:00 [entrez] AID - 10.1038/icb.1986.13 [doi] PST - ppublish SO - Aust J Exp Biol Med Sci. 1986 Apr;64 ( Pt 2):119-26. doi: 10.1038/icb.1986.13.