PMID- 29434341 OWN - NLM STAT- MEDLINE DCOM- 20190520 LR - 20230210 IS - 1530-0285 (Electronic) IS - 0893-3952 (Linking) VI - 31 IP - 6 DP - 2018 Jun TI - Hyalinizing clear cell carcinoma of the bronchial glands: presentation of three cases and pathological comparisons with salivary gland counterparts and bronchial mucoepidermoid carcinomas. PG - 923-933 LID - 10.1038/s41379-018-0025-7 [doi] AB - Hyalinizing clear cell carcinoma of the bronchial glands is a very rare tumor. Since only five reports describing six tumors have been published to date, only a little is known about specific histologic findings and clinical features. Because of its rarity, hyalinizing clear cell carcinoma has not been described in the latest WHO classification of pulmonary tumors yet. Here we present three cases of bronchial hyalinizing clear cell carcinomas, confirmed by both fluorescence in situ hybridization (FISH) and RT-PCR, focusing on histologic and immunohistochemical characteristics in a comparison with three cases of salivary gland origin. In addition, we compared immunohistochemical features with bronchial mucoepidermoid carcinoma, a lesion that needs to be taken into account in differential diagnosis of hyalinizing clear cell carcinoma. All our bronchial hyalinizing clear cell carcinoma cases were surgically resected. Histologically, tumor cells showed clear to eosinophilic cytoplasm with hyalinizing stroma in various proportions, resembling those of salivary gland origin. Immunohistochemically, tumor cells were positive for CK7, CK5/6, p40, p63, and ATF1, while they were negative for TTF1, Napsin A, HMB45, and SOX10. The CK5/6 staining pattern varied in mucoepidermoid carcinomas, while that of hyalinizing clear cell carcinoma was uniformly positive. FISH revealed EWSR1-ATF1 fusion, and RT-PCR with sequencing confirmed specificity of the chimeric gene for hyalinizing clear cell carcinoma. Clinically, bronchial hyalinizing clear cell carcinoma was characterized by occurrence in the fourth to sixth decades, no link with smoking history, and a predilection for the right lung, in line with previous reports. In summary, our study confirmed that the bronchial hyalinizing clear cell carcinoma is a histologically and genetically identical tumor to that of salivary gland origin, and that gene rearrangement analysis can play a critical role in distinction from mucoepidermoid carcinoma. FAU - Takamatsu, Manabu AU - Takamatsu M AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Sato, Yukiko AU - Sato Y AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Muto, Mariko AU - Muto M AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Nagano, Hiroko AU - Nagano H AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Ninomiya, Hironori AU - Ninomiya H AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Sakakibara, Rie AU - Sakakibara R AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Baba, Satoko AU - Baba S AD - Pathology Project for Molecular Targets, The Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Sakata, Seiji AU - Sakata S AD - Pathology Project for Molecular Targets, The Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Takeuchi, Kengo AU - Takeuchi K AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. AD - Pathology Project for Molecular Targets, The Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Okumura, Sakae AU - Okumura S AD - Department of Thoracic Surgery, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. FAU - Ishikawa, Yuichi AU - Ishikawa Y AD - Division of Pathology, The Cancer Institute; Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, 135-8550, Japan. ishikawa@jfcr.or.jp. LA - eng PT - Journal Article DEP - 20180212 PL - United States TA - Mod Pathol JT - Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc JID - 8806605 RN - 0 (Biomarkers, Tumor) SB - IM MH - Adenocarcinoma, Clear Cell/metabolism/*pathology MH - Adult MH - Aged, 80 and over MH - Biomarkers, Tumor/metabolism MH - Bronchial Neoplasms/metabolism/*pathology MH - Carcinoma, Mucoepidermoid/metabolism/*pathology MH - Female MH - Humans MH - Immunohistochemistry MH - Male MH - Middle Aged MH - Salivary Gland Neoplasms/metabolism/*pathology EDAT- 2018/02/13 06:00 MHDA- 2019/05/21 06:00 CRDT- 2018/02/14 06:00 PHST- 2017/10/17 00:00 [received] PHST- 2017/12/17 00:00 [accepted] PHST- 2017/12/12 00:00 [revised] PHST- 2018/02/13 06:00 [pubmed] PHST- 2019/05/21 06:00 [medline] PHST- 2018/02/14 06:00 [entrez] AID - S0893-3952(22)01493-4 [pii] AID - 10.1038/s41379-018-0025-7 [doi] PST - ppublish SO - Mod Pathol. 2018 Jun;31(6):923-933. doi: 10.1038/s41379-018-0025-7. Epub 2018 Feb 12.