PMID- 29434550
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 9
DP  - 2018
TI  - Select Skeletal Muscle mRNAs Related to Exercise Adaptation Are Minimally 
      Affected by Different Pre-exercise Meals that Differ in Macronutrient Profile.
PG  - 28
LID - 10.3389/fphys.2018.00028 [doi]
LID - 28
AB  - Background: Substantial research has been done on the impact of carbohydrate and 
      fat availability on endurance exercise adaptation, though its role in the acute 
      adaptive response to resistance exercise has yet to be fully characterized. 
      Purpose: We aimed to assess the effects of a pre-resistance exercise isocaloric 
      mixed meal containing different amounts of carbohydrates and fat, on 
      post-resistance exercise gene expression associated with muscle adaptation. 
      Methods: Thirteen young (age 21.2 +/- 1.6 year), recreationally trained (VO(2max) 
      51.3 +/- 4.8 ml/kg/min) men undertook an aerobic exercise session of 90-min 
      continuous cycling (70% VO(2max)) in the morning with pre- and post-exercise 
      protein ingestion (10 and 15 g casein in a 500 ml beverage pre- and 
      post-exercise, respectively). Subjects then rested for 2 h and were provided with 
      a meal consisting of either 3207 kJ; 52 g protein; 51 g fat; and 23 g 
      carbohydrate (FAT) or 3124 kJ; 53 g protein; 9 g fat; and 109 g carbohydrate 
      (CHO). Two hours after the meal, subjects completed 5 x 8 repetitions (80% 1-RM) 
      for both bilateral leg press and leg extension directly followed by 25 g of whey 
      protein (500 ml beverage). Muscle biopsies were obtained from the vastus 
      lateralis at baseline (morning) and 1 and 3 h post-resistance exercise 
      (afternoon) to determine intramuscular mRNA response. Results: Muscle glycogen 
      levels were significantly decreased post-resistance exercise, without any 
      differences between conditions. Plasma free fatty acids increased significantly 
      after the mixed meal in the FAT condition, while glucose and insulin were higher 
      in the CHO condition. However, PDK4 mRNA quantity was significantly higher in the 
      FAT condition at 3 h post-resistance exercise compared to CHO. HBEGF, INSIG1, 
      MAFbx, MURF1, SIRT1, and myostatin responded solely as a result of exercise 
      without any differences between the CHO and FAT group. FOXO3A, IGF-1, PGC-1alpha, and 
      VCP expression levels remained unchanged over the course of the day. Conclusion: 
      We conclude that mRNA quantity associated with muscle adaptation after resistance 
      exercise is not affected by a difference in pre-exercise nutrient availability. 
      PDK4 was differentially expressed between CHO and FAT groups, suggesting a 
      potential shift toward fat oxidation and reduced glucose oxidation in the FAT 
      group.
FAU - Knuiman, Pim
AU  - Knuiman P
AD  - Division of Human Nutrition, Wageningen University and Research, Wageningen, 
      Netherlands.
FAU - Hopman, Maria T E
AU  - Hopman MTE
AD  - Division of Human Nutrition, Wageningen University and Research, Wageningen, 
      Netherlands.
AD  - Department of Physiology, Radboud University Medical Centre, Nijmegen, 
      Netherlands.
FAU - Wouters, Jeroen A
AU  - Wouters JA
AD  - Centre for Sporting Excellence and Education, Sportcentre Papendal, Arnhem, 
      Netherlands.
FAU - Mensink, Marco
AU  - Mensink M
AD  - Division of Human Nutrition, Wageningen University and Research, Wageningen, 
      Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20180126
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC5791349
OTO - NOTNLM
OT  - endurance exercise
OT  - gene expression
OT  - humans
OT  - nutrient availability
OT  - resistance exercise
EDAT- 2018/02/13 06:00
MHDA- 2018/02/13 06:01
PMCR- 2018/01/26
CRDT- 2018/02/14 06:00
PHST- 2017/10/27 00:00 [received]
PHST- 2018/01/09 00:00 [accepted]
PHST- 2018/02/14 06:00 [entrez]
PHST- 2018/02/13 06:00 [pubmed]
PHST- 2018/02/13 06:01 [medline]
PHST- 2018/01/26 00:00 [pmc-release]
AID - 10.3389/fphys.2018.00028 [doi]
PST - epublish
SO  - Front Physiol. 2018 Jan 26;9:28. doi: 10.3389/fphys.2018.00028. eCollection 2018.