PMID- 29434833
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 2
DP  - 2018 Feb
TI  - Piperlongumine induces apoptosis and autophagy in leukemic cells through 
      targeting the PI3K/Akt/mTOR and p38 signaling pathways.
PG  - 1423-1428
LID - 10.3892/ol.2017.7498 [doi]
AB  - Piperlongumine is an alkaloid compound extracted from Piper longum L. It is a 
      chemical substance with various pharmacological effects and medicinal value, 
      including anti-tumor, lipid metabolism regulatory, antiplatelet aggregation and 
      analgesic properties. The present study aimed to understand whether 
      piperlongumine induces the apoptosis and autophagy of leukemic cells, and to 
      identify the mechanism involved. Cell viability and autophagy were detected using 
      MTT, phenazine methyl sulfate and trypan blue exclusion assays. The apoptosis 
      rate was calculated using flow cytometry. The protein expression levels of 
      microtubule-associated protein 1A/1B-light chain 3, Akt and mechanistic target of 
      rapamycin (mTOR) were measured using western blotting. The cell growth of 
      leukemic cells was completely inhibited following treatment with piperlongumine, 
      and marked apoptosis was also induced. Dead cells as a result of autophagy were 
      stained using immunofluorescence and observed under a light microscope. 
      Phosphoinositide 3-kinase (PI3K)/Akt/mTOR signaling was suppressed by treatment 
      with piperlongumine, while p38 signaling and caspase-3 activity were induced by 
      treatment with piperlongumine. It was concluded that piperlongumine induces 
      apoptosis and autophagy in leukemic cells through targeting the PI3K/Akt/mTOR and 
      p38 signaling pathways.
FAU - Wang, Hongfei
AU  - Wang H
AD  - Department of Intensive Care Unit, Tianjin First Center Hospital, Nankai, Tianjin 
      300192, P.R. China.
FAU - Wang, Yongqiang
AU  - Wang Y
AD  - Department of Intensive Care Unit, Tianjin First Center Hospital, Nankai, Tianjin 
      300192, P.R. China.
FAU - Gao, Hongmei
AU  - Gao H
AD  - Department of Intensive Care Unit, Tianjin First Center Hospital, Nankai, Tianjin 
      300192, P.R. China.
FAU - Wang, Bing
AU  - Wang B
AD  - Department of Intensive Care Unit, Tianjin First Center Hospital, Nankai, Tianjin 
      300192, P.R. China.
FAU - Dou, Lin
AU  - Dou L
AD  - Department of Intensive Care Unit, Tianjin First Center Hospital, Nankai, Tianjin 
      300192, P.R. China.
FAU - Li, Yin
AU  - Li Y
AD  - Department of Intensive Care Unit, Tianjin First Center Hospital, Nankai, Tianjin 
      300192, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171129
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5774427
OTO - NOTNLM
OT  - PI3K/Akt/mTOR
OT  - autophagy
OT  - leukemic cells
OT  - p38
OT  - piperlongumine
EDAT- 2018/02/13 06:00
MHDA- 2018/02/13 06:01
PMCR- 2017/11/29
CRDT- 2018/02/14 06:00
PHST- 2015/11/14 00:00 [received]
PHST- 2017/06/16 00:00 [accepted]
PHST- 2018/02/14 06:00 [entrez]
PHST- 2018/02/13 06:00 [pubmed]
PHST- 2018/02/13 06:01 [medline]
PHST- 2017/11/29 00:00 [pmc-release]
AID - OL-0-0-7498 [pii]
AID - 10.3892/ol.2017.7498 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Feb;15(2):1423-1428. doi: 10.3892/ol.2017.7498. Epub 2017 Nov 
      29.