PMID- 29435370
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220408
IS  - 2090-3057 (Print)
IS  - 2090-3065 (Electronic)
IS  - 2090-3057 (Linking)
VI  - 2017
DP  - 2017
TI  - Analysis of Aminoglycoside Modifying Enzyme Genes Responsible for High-Level 
      Aminoglycoside Resistance among Enterococcal Isolates.
PG  - 3256952
LID - 10.1155/2017/3256952 [doi]
LID - 3256952
AB  - Enzymatic modification results in high-level resistance to aminoglycoside (HLAR), 
      which eliminates the synergistic bactericidal effect of combined exposure to a 
      cell wall-active agent and an aminoglycoside. So aim of the study was to 
      determine prevalence of HLAR enterococcal isolate and to study distribution of 
      aminoglycoside modifying enzyme genes in them. A total of 100 nonrepeat isolates 
      of enterococci from various clinical samples were analyzed. As per Clinical and 
      Laboratory Standards Institute guidelines enterococci were screened for HLAR by 
      Kirby-Bauer disc diffusion method. Minimum inhibitory concentration of all 
      isolates for gentamicin and streptomycin was determined by E-test. Multiplex 
      polymerase chain reaction (PCR) was carried out for HLAR enterococcal isolates to 
      identify aminoglycoside modifying enzymes genes responsible for resistance. 60% 
      isolates were found to be high-level gentamicin resistant (HLGR) whereas 45% 
      isolates were found to be high-level streptomycin resistant (HLSR). By multiplex 
      PCR 80% HLGR isolates carried bifunctional aminoglycoside modifying enzyme gene 
      aac(6')-Ie-aph(2'')-Ia whereas 18 out of 45 high-level streptomycin resistant, 
      that is, 40%, isolates carried aph(3')-IIIa. However, aph(2'')-Ib, aph(2'')-Ic, 
      aph(2'')-Id, and ant(4')-Ia genes which encode other aminoglycosides modifying 
      enzymes were not detected. Bifunctional aminoglycoside modifying enzyme gene 
      aac(6')-Ie-aph(2'')-Ia is the predominant gene responsible for HLAR.
FAU - Shete, Vishal
AU  - Shete V
AUID- ORCID: 0000-0002-6202-8784
AD  - Department of Microbiology, Armed Forces Medical College, Pune, India.
FAU - Grover, Naveen
AU  - Grover N
AUID- ORCID: 0000-0002-4083-761X
AD  - Department of Microbiology, Armed Forces Medical College, Pune, India.
FAU - Kumar, Mahadevan
AU  - Kumar M
AUID- ORCID: 0000-0003-1656-1649
AD  - Department of Microbiology, Armed Forces Medical College, Pune, India.
LA  - eng
PT  - Journal Article
DEP - 20171224
PL  - Egypt
TA  - J Pathog
JT  - Journal of pathogens
JID - 101571433
PMC - PMC5757141
EDAT- 2018/02/13 06:00
MHDA- 2018/02/13 06:01
PMCR- 2017/12/24
CRDT- 2018/02/14 06:00
PHST- 2017/08/14 00:00 [received]
PHST- 2017/10/17 00:00 [accepted]
PHST- 2018/02/14 06:00 [entrez]
PHST- 2018/02/13 06:00 [pubmed]
PHST- 2018/02/13 06:01 [medline]
PHST- 2017/12/24 00:00 [pmc-release]
AID - 10.1155/2017/3256952 [doi]
PST - ppublish
SO  - J Pathog. 2017;2017:3256952. doi: 10.1155/2017/3256952. Epub 2017 Dec 24.