PMID- 29435931
OWN - NLM
STAT- MEDLINE
DCOM- 20181231
LR  - 20181231
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1733
DP  - 2018
TI  - In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, 
      Invasion, and Metastasis.
PG  - 159-171
LID - 10.1007/978-1-4939-7601-0_13 [doi]
AB  - MicroRNAs (miRNAs) are small noncoding, single-stranded RNAs consisting of 20-24 
      nucleotides (Bartel, Cell 116:281-297, 2004), which regulate target genes 
      expression by interacting with 3'-untranslated regions (3'-UTRs) of target mRNAs, 
      leading to translation repression or mRNA degradation (Filipowicz et al., Nat Rev 
      Genet 9:102-114, 2008; Nilsen, Trends Genet 23:243-249, 2007). Accumulating 
      evidence has elucidated them as oncogenes or tumor suppressors in cancers such as 
      hepatocellular carcinoma, breast cancer, and lung cancer (Liu et al., 
      Gastroenterology 136:683-693, 2009; Yu et al., Cell 131:1109-1123, 2007; Zhou et 
      al., Sci Rep 7:42680, 2017; Iorio and Croce, Carcinogenesis 33:1126-1133, 2012). 
      MiRNAs are involved in various biological processes, including cell proliferation 
      (Liu et al., Mol Cancer Res 11:1314-1325, 2013), differentiation (Liu et al., Mol 
      Cancer Res 11:1314-1325, 2013), apoptosis (Pan et al., Oncol Res 24:429-435, 
      2016), invasion and metastasis (Liu et al., Nat Commun 8:14270, 2017). 
      Understanding the role of miRNAs in tumor gives new perspective on cancer 
      diagnosis and therapy (Rupaimoole and Slack, Nat Rev Drug Discov 16:203-222, 
      2017; Berindan-Neagoe et al., CA Cancer J Clin 64:311-336, 2014). This chapter 
      will focus on the in vitro methods for identifying miRNAs roles in cell 
      proliferation, invasion, and metastasis in tumor development, which includes 
      CCK-8 assay, Wound Healing assay, and Transwell assay.
FAU - Xu, Jian
AU  - Xu J
AD  - Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China. xu_Jian79@163.com.
FAU - Xiao, Xuelian
AU  - Xiao X
AD  - Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
FAU - Yang, Daheng
AU  - Yang D
AD  - Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Cell Movement/genetics
MH  - Cell Proliferation
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Neoplasm Metastasis
MH  - Neoplasms/*genetics/pathology
MH  - Real-Time Polymerase Chain Reaction
MH  - Transfection
OTO - NOTNLM
OT  - Invasion
OT  - Metastasis
OT  - MiRNA
OT  - Proliferation
EDAT- 2018/02/13 06:00
MHDA- 2019/01/01 06:00
CRDT- 2018/02/14 06:00
PHST- 2018/02/14 06:00 [entrez]
PHST- 2018/02/13 06:00 [pubmed]
PHST- 2019/01/01 06:00 [medline]
AID - 10.1007/978-1-4939-7601-0_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1733:159-171. doi: 10.1007/978-1-4939-7601-0_13.