PMID- 29436630
OWN - NLM
STAT- MEDLINE
DCOM- 20180823
LR  - 20220409
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 52
IP  - 4
DP  - 2018 Apr
TI  - Silencing of B7-H4 suppresses the tumorigenicity of the MGC-803 human gastric 
      cancer cell line and promotes cell apoptosis via the mitochondrial signaling 
      pathway.
PG  - 1267-1276
LID - 10.3892/ijo.2018.4274 [doi]
AB  - B7-H4 is a transmembrane protein which is a member of the B7 superfamily. It is 
      overexpressed in various types of cancer, including gastric cancer. However, the 
      effects of B7-H4 on the tumorigenicity of gastric cancer and the underlying 
      mechanisms have not yet been fully explored. Thus, the aim of this study was to 
      examine the effects of B7-H4 on the tumorigenicity of gastric cancer cells and to 
      elucidate the underlying mechanisms. For this purpose, B7-H4 expression in 
      gastric cancer tissues was detected by immunohistochemical staining. The effects 
      of B7-H4 on the biological behavior of the MGC-803 human gastric cancer cell line 
      were examined by Cell Counting kit-8 (CCK-8) assay, cell cycle analysis, wound 
      healing assay, Annexin V/propidium iodide staining and terminal deoxynucleotidyl 
      transferase dUTP nick-end labeling (TUNEL) assay. Moreover, the expression levels 
      of apoptotic markers, such as cleaved caspase‑3, cleaved caspase‑9, Bcl-2 and Bax 
      were examined by western blot analysis. Immunohistochemical staining revealed 
      that a high expression of B7-H4 was found in about 41.8% of tissues obtained from 
      patients with gastric cancer. Comparative analysis revealed that B7-H4 expression 
      significantly correlated with lymph node metastasis and the TNM stage. The 
      results of CCK-8 assay, cell cycle analysis, wound healing assay, 
      Annexin V/propidium iodide staining assay and TUNEL assay all demonstrated that 
      the silencing of B7-H4 by small interfering RNA decreased cell proliferation, 
      suppressed cell motility, and induced cell cycle arrest and the apoptosis of 
      MGC-803 human gastric cancer cells. Furthermore, the results of western blot 
      analysis indicated that the downregulation of B7-H4 induced the apoptosis of the 
      MGC-803 cells via the mitochondrial signaling pathway through the activation of 
      caspase‑3 and caspase‑9, and by altering the Bax/Bcl-2 ratio in a manner that 
      favored apoptosis. Based on the findings on human gastric cancer cell line 
      MGC-803, the findings of this study suggested that B7-H4 may have the potential 
      to be a valuable prognostic marker and a target for individualized therapies for 
      gastric cancer. However, further investigations are required in order to confirm 
      our findings on a larger scale.
FAU - Zhou, Donghui
AU  - Zhou D
AD  - Department of Oncology, The First Affiliated Hospital, Zhejiang University School 
      of Medicine, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Zhou, Yong
AU  - Zhou Y
AD  - Department of Oncology, The First Affiliated Hospital, Zhejiang University School 
      of Medicine, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Li, Chao
AU  - Li C
AD  - Department of Oncology, The First Affiliated Hospital, Zhejiang University School 
      of Medicine, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Yang, Lina
AU  - Yang L
AD  - Department of Oncology, The Affiliated Dongnan Hospital of Xiamen University, 
      Zhangzhou, Fujian 363000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180213
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (V-Set Domain-Containing T-Cell Activation Inhibitor 1)
RN  - 0 (VTCN1 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptosis/physiology
MH  - Carcinogenesis/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/physiology
MH  - Cell Proliferation/physiology
MH  - Female
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Signal Transduction/physiology
MH  - Stomach Neoplasms/*pathology
MH  - V-Set Domain-Containing T-Cell Activation Inhibitor 1/*metabolism
EDAT- 2018/02/14 06:00
MHDA- 2018/08/24 06:00
CRDT- 2018/02/14 06:00
PHST- 2017/09/18 00:00 [received]
PHST- 2018/01/19 00:00 [accepted]
PHST- 2018/02/14 06:00 [pubmed]
PHST- 2018/08/24 06:00 [medline]
PHST- 2018/02/14 06:00 [entrez]
AID - 10.3892/ijo.2018.4274 [doi]
PST - ppublish
SO  - Int J Oncol. 2018 Apr;52(4):1267-1276. doi: 10.3892/ijo.2018.4274. Epub 2018 Feb 
      13.