PMID- 29437277
OWN - NLM
STAT- MEDLINE
DCOM- 20190816
LR  - 20190816
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 18
IP  - 12
DP  - 2018 Jun
TI  - Identification of Native and Posttranslationally Modified HLA-B*57:01-Restricted 
      HIV Envelope Derived Epitopes Using Immunoproteomics.
PG  - e1700253
LID - 10.1002/pmic.201700253 [doi]
AB  - The recognition of pathogen-derived peptides by T lymphocytes is the cornerstone 
      of adaptive immunity, whereby intracellular antigens are degraded in the cytosol 
      and short peptides assemble with class I human leukocyte antigen (HLA) molecules 
      in the ER. These peptide-HLA complexes egress to the cell surface and are 
      scrutinized by cytotoxic CD8+ T-cells leading to the eradication of the infected 
      cell. Here, naturally presented HLA-B*57:01 bound peptides derived from the 
      envelope protein of the human immunodeficiency virus (HIVenv) are identified. 
      HIVenv peptides are present at a very small percentage of the overall HLA-B*57:01 
      peptidome (<0.1%) and both native and posttranslationally modified forms of two 
      distinct HIV peptides are identified. Notably, a peptide bearing a natively 
      encoded C-terminal tryptophan residue is also present in a modified form 
      containing a kynurenine residue. Kynurenine is a major product of tryptophan 
      catabolism and is abundant during inflammation and infection. Binding of these 
      peptides at a molecular level and their immunogenicity in preliminary functional 
      studies are examined. Modest immune responses are observed to the modified HIVenv 
      peptide, highlighting a potential role for kynurenine-modified peptides in the 
      immune response to HIV and other viral infections.
CI  - (c) 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Ramarathinam, Sri H
AU  - Ramarathinam SH
AUID- ORCID: 0000-0002-2787-1282
AD  - Infection and Immunity Program, Biomedicine Discovery Institute & Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
FAU - Gras, Stephanie
AU  - Gras S
AD  - Infection and Immunity Program, Biomedicine Discovery Institute & Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
AD  - ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, 
      Clayton, Australia.
FAU - Alcantara, Sheilajen
AU  - Alcantara S
AD  - Department of Microbiology and Immunology, Peter Doherty Institute for Infection 
      and Immunity, The University of Melbourne, Parkville, Australia.
FAU - Yeung, Amanda W S
AU  - Yeung AWS
AD  - Mechanisms of Disease and Translational Medicine, Department of Pathology, School 
      of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, 
      Australia.
FAU - Mifsud, Nicole A
AU  - Mifsud NA
AD  - Infection and Immunity Program, Biomedicine Discovery Institute & Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
FAU - Sonza, Secondo
AU  - Sonza S
AD  - Department of Microbiology and Immunology, Peter Doherty Institute for Infection 
      and Immunity, The University of Melbourne, Parkville, Australia.
FAU - Illing, Patricia T
AU  - Illing PT
AD  - Infection and Immunity Program, Biomedicine Discovery Institute & Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
FAU - Glaros, Elias N
AU  - Glaros EN
AD  - Mechanisms of Disease and Translational Medicine, Department of Pathology, School 
      of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, 
      Australia.
FAU - Center, Robert J
AU  - Center RJ
AD  - Department of Microbiology and Immunology, Peter Doherty Institute for Infection 
      and Immunity, The University of Melbourne, Parkville, Australia.
AD  - Burnet Institute, Melbourne, Australia.
FAU - Thomas, Shane R
AU  - Thomas SR
AD  - Mechanisms of Disease and Translational Medicine, Department of Pathology, School 
      of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, 
      Australia.
FAU - Kent, Stephen J
AU  - Kent SJ
AD  - Department of Microbiology and Immunology, Peter Doherty Institute for Infection 
      and Immunity, The University of Melbourne, Parkville, Australia.
AD  - Melbourne Sexual Health Centre, Central Clinical School, Monash University, 
      Melbourne, Australia.
AD  - ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, 
      University of Melbourne, Parkville, Australia.
FAU - Ternette, Nicola
AU  - Ternette N
AUID- ORCID: 0000-0002-9283-0743
AD  - The Jenner Institute, Target Discovery Institute Mass Spectrometry Laboratory, 
      University of Oxford, Oxford, UK.
FAU - Purcell, Damian F J
AU  - Purcell DFJ
AD  - Department of Microbiology and Immunology, Peter Doherty Institute for Infection 
      and Immunity, The University of Melbourne, Parkville, Australia.
FAU - Rossjohn, Jamie
AU  - Rossjohn J
AD  - Infection and Immunity Program, Biomedicine Discovery Institute & Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
AD  - ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, 
      Clayton, Australia.
AD  - Institute of Infection and Immunity, Cardiff University School of Medicine, 
      Cardiff, UK.
FAU - Purcell, Anthony W
AU  - Purcell AW
AUID- ORCID: 0000-0003-0532-8331
AD  - Infection and Immunity Program, Biomedicine Discovery Institute & Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180410
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, env)
RN  - 0 (HIV Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B57 antigen)
CIN - Proteomics. 2018 Jun;18(12):e1800041. PMID: 29513933
MH  - B-Lymphocytes/*immunology/virology
MH  - Cells, Cultured
MH  - Epitopes/*immunology/metabolism
MH  - Gene Products, env/*immunology/metabolism
MH  - HIV Antigens/*immunology/metabolism
MH  - HIV Infections/immunology/virology
MH  - HIV-1/*immunology
MH  - HLA-B Antigens/chemistry/*immunology/metabolism
MH  - Humans
MH  - *Protein Processing, Post-Translational
OTO - NOTNLM
OT  - HLA-B*57:01
OT  - human immunodeficiency virus
OT  - immunopeptidome
OT  - kynurenine
OT  - posttranslational modification
EDAT- 2018/02/14 06:00
MHDA- 2019/08/17 06:00
CRDT- 2018/02/14 06:00
PHST- 2017/10/27 00:00 [received]
PHST- 2018/01/29 00:00 [revised]
PHST- 2018/02/14 06:00 [pubmed]
PHST- 2019/08/17 06:00 [medline]
PHST- 2018/02/14 06:00 [entrez]
AID - 10.1002/pmic.201700253 [doi]
PST - ppublish
SO  - Proteomics. 2018 Jun;18(12):e1700253. doi: 10.1002/pmic.201700253. Epub 2018 Apr 
      10.