PMID- 29439856
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20181015
IS  - 1440-1592 (Electronic)
IS  - 1323-8930 (Linking)
VI  - 67
IP  - 3
DP  - 2018 Jul
TI  - The optimal age for epicutaneous sensitization following tape-stripping in BALB/c 
      mice.
PG  - 380-387
LID - S1323-8930(18)30003-0 [pii]
LID - 10.1016/j.alit.2018.01.003 [doi]
AB  - BACKGROUND: Direct contact of food proteins with eczematous lesions is thought to 
      be the main cause of epicutaneous sensitization. To further investigate the 
      development and pathogenesis of food allergy in vivo, a good mouse model of 
      epicutaneous sensitization is needed. However, a fundamental problem in that 
      regard is that the optimal age for epicutaneous sensitization of mice is unknown. 
      In this study, we attempted to elucidate that optimal age. METHODS: Dorsal skin 
      of wild-type BALB/c female mice (1, 3, 8 and 24 weeks old) was shaved, depilated 
      and tape-stripped. A Finn chamber containing a 20-mul-aliquot of 20-mg/ml (OVA) 
      was applied to the tape-stripped skin on 3 consecutive days/week, for 3 weeks. 
      The body temperature was measured after intraperitoneal OVA challenge. Serum 
      OVA-specific IgE titers and OVA-induced cytokine production by spleen cells were 
      measured by ELISA. Dendritic cells (DCs) that migrated to the draining lymph 
      nodes were quantified by FITC-labeled OVA and flow cytometry. The mRNA expression 
      levels in the dorsal skin were measured by qPCR. RESULTS: A significant 
      age-dependent body temperature decline was observed after OVA challenge. The 
      serum OVA-specific IgE titer, OVA-induced cytokine production (i.e., IL-4, IL-5 
      and IL-13) by spleen cells, and number of FITC-OVA-engulfing DCs increased with 
      age. In addition, mRNA for IL-33, but not TSLP or IL-25, was significantly 
      induced in the skin by tape-stripping and increased with age. CONCLUSIONS: 
      Twenty-four-week-old mice showed the greatest DC migration, Th2 polarization, IgE 
      production and body temperature decline. Skin-derived IL-33 is likely to play key 
      roles in those changes.
CI  - Copyright (c) 2018 Japanese Society of Allergology. Production and hosting by 
      Elsevier B.V. All rights reserved.
FAU - Tamari, Masato
AU  - Tamari M
AD  - Department of Allergy and Clinical Immunology, National Research Institute for 
      Child Health and Development, Tokyo, Japan; Department of Pediatrics, Jikei 
      University School of Medicine, Tokyo, Japan.
FAU - Orimo, Keisuke
AU  - Orimo K
AD  - Department of Allergy and Clinical Immunology, National Research Institute for 
      Child Health and Development, Tokyo, Japan; First Department of Medicine, Tokyo 
      Women's Medical University, Tokyo, Japan.
FAU - Motomura, Kenichiro
AU  - Motomura K
AD  - Department of Allergy and Clinical Immunology, National Research Institute for 
      Child Health and Development, Tokyo, Japan.
FAU - Arae, Ken
AU  - Arae K
AD  - Department of Immunology, Faculty of Health Sciences, Kyorin University, Tokyo, 
      Japan.
FAU - Matsuda, Akio
AU  - Matsuda A
AD  - Department of Allergy and Clinical Immunology, National Research Institute for 
      Child Health and Development, Tokyo, Japan.
FAU - Nakae, Susumu
AU  - Nakae S
AD  - Laboratory of Systems Biology, Center for Experimental Medicine and Systems 
      Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Saito, Hirohisa
AU  - Saito H
AD  - Department of Allergy and Clinical Immunology, National Research Institute for 
      Child Health and Development, Tokyo, Japan.
FAU - Morita, Hideaki
AU  - Morita H
AD  - Department of Allergy and Clinical Immunology, National Research Institute for 
      Child Health and Development, Tokyo, Japan.
FAU - Matsumoto, Kenji
AU  - Matsumoto K
AD  - Department of Allergy and Clinical Immunology, National Research Institute for 
      Child Health and Development, Tokyo, Japan. Electronic address: 
      matsumoto-k@ncchd.go.jp.
LA  - eng
PT  - Journal Article
DEP - 20180210
PL  - England
TA  - Allergol Int
JT  - Allergology international : official journal of the Japanese Society of 
      Allergology
JID - 9616296
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Administration, Cutaneous
MH  - Animals
MH  - *Disease Models, Animal
MH  - Female
MH  - Food Hypersensitivity/*immunology
MH  - Immunization/*methods
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/*administration & dosage/immunology/toxicity
MH  - Skin/drug effects/immunology
OTO - NOTNLM
OT  - Epicutaneous sensitization
OT  - Food allergy
OT  - IL-33
OT  - Mouse model
OT  - Tape stripping
EDAT- 2018/02/15 06:00
MHDA- 2018/10/16 06:00
CRDT- 2018/02/15 06:00
PHST- 2017/10/23 00:00 [received]
PHST- 2017/11/29 00:00 [revised]
PHST- 2017/12/18 00:00 [accepted]
PHST- 2018/02/15 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2018/02/15 06:00 [entrez]
AID - S1323-8930(18)30003-0 [pii]
AID - 10.1016/j.alit.2018.01.003 [doi]
PST - ppublish
SO  - Allergol Int. 2018 Jul;67(3):380-387. doi: 10.1016/j.alit.2018.01.003. Epub 2018 
      Feb 10.