PMID- 29443890
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240214
IS  - 2073-4425 (Print)
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 9
IP  - 2
DP  - 2018 Feb 14
TI  - Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed 
      Flow Cell for Detection of Hepatic Oval Cells (HOCs).
LID - 10.3390/genes9020089 [doi]
LID - 89
AB  - Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem 
      cells that are found in a small population in the liver after hepatocyte 
      proliferation is inhibited. Due to their small number, isolation and capture of 
      these cells constitute a challenging task for immunosensor technology. This work 
      describes the development of a 3D-printed continuous flow system and exploits 
      disposable screen-printed electrodes for the rapid detection of HOCs that 
      over-express the OV6 marker on their membrane. Multiwall carbon nanotube (MWCNT) 
      electrodes have a chitosan film that serves as a scaffold for the immobilization 
      of oval cell marker antibodies (anti-OV6-Ab), which enhance the sensitivity of 
      the biomarker and makes the designed sensor specific for oval cells. The 
      developed sensor can be easily embedded into the 3D-printed flow cell to allow 
      cells to be exposed continuously to the functionalized surface. The continuous 
      flow is intended to increase capture of most of the target cells in the specimen. 
      Contact angle measurements were performed to characterize the nature and quality 
      of the modified sensor surface, and electrochemical measurements (cyclic 
      voltammetry (CV) and square wave voltammetry (SWV)) were performed to confirm the 
      efficiency and selectivity of the fabricated sensor to detect HOCs. The proposed 
      method is valuable for capturing rare cells and could provide an effective tool 
      for cancer diagnosis and detection.
FAU - Damiati, Samar
AU  - Damiati S
AUID- ORCID: 0000-0002-6747-0408
AD  - Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), 
      Jeddah 21589, Saudi Arabia. sdamiati@kau.edu.sa.
AD  - Institute for Synthetic Bioarchitecture, Department of Nanobiotechnology, 
      University of Natural Resources and Life Sciences, 1190 Vienna, Austria. 
      sdamiati@kau.edu.sa.
FAU - Peacock, Martin
AU  - Peacock M
AD  - Zimmer and Peacock Ltd., Royston SG8 9JL, UK. martinpeacock@zimmerpeacock.com.
FAU - Leonhardt, Stefan
AU  - Leonhardt S
AD  - Institute of Medical and Polymer Engineering, Technical University of Munich 
      (TUM), 85748 Garching, Germany. stefan.leonhardt@tum.de.
FAU - Damiati, Laila
AU  - Damiati L
AD  - Centre for Cell Engineering, University of Glasgow, Glasgow G12 8QQ, UK. 
      l.damiati.1@research.gla.ac.uk.
AD  - Department of Biology, Jeddah University, Jeddah 23218, Saudi Arabia. 
      l.damiati.1@research.gla.ac.uk.
FAU - Baghdadi, Mohammed A
AU  - Baghdadi MA
AD  - Research Centre, King Faisal Specialist Hospital & Research Centre, Jeddah 21499, 
      Saudi Arabia. m-baghdadi@hotmail.com.
FAU - Becker, Holger
AU  - Becker H
AUID- ORCID: 0000-0002-7678-2518
AD  - Microfluidic ChipShop Gmbh, 07747 Jena, Germany. hb@microfluidic-chipshop.com.
FAU - Kodzius, Rimantas
AU  - Kodzius R
AUID- ORCID: 0000-0001-9417-8894
AD  - Mathematics and Natural Sciences Department, The American University of Iraq, 
      Sulaimani, Sulaymaniyah 46001, Iraq. kodzius@envirola.com.
AD  - Materials Genome Institute, Shanghai University, Shanghai 200444, China. 
      kodzius@envirola.com.
AD  - Faculty of Medicine, Ludwig Maximilian University of Munich (LMU), 80539 Munich, 
      Germany. kodzius@envirola.com.
AD  - Faculty of Medicine, Technical University of Munich (TUM), 81675 Munich, Germany. 
      kodzius@envirola.com.
FAU - Schuster, Bernhard
AU  - Schuster B
AD  - Institute for Synthetic Bioarchitecture, Department of Nanobiotechnology, 
      University of Natural Resources and Life Sciences, 1190 Vienna, Austria. 
      bernhard.schuster@boku.ac.at.
LA  - eng
GR  - P 29399/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
DEP - 20180214
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
PMC - PMC5852585
OTO - NOTNLM
OT  - 3D-printed flow-cell
OT  - cancer diagnosis
OT  - hepatic oval cells
OT  - multiwall carbon nanotubes
COIS- The authors declare no conflict of interest.
EDAT- 2018/02/15 06:00
MHDA- 2018/02/15 06:01
PMCR- 2018/02/01
CRDT- 2018/02/15 06:00
PHST- 2018/01/08 00:00 [received]
PHST- 2018/02/06 00:00 [revised]
PHST- 2018/02/06 00:00 [accepted]
PHST- 2018/02/15 06:00 [entrez]
PHST- 2018/02/15 06:00 [pubmed]
PHST- 2018/02/15 06:01 [medline]
PHST- 2018/02/01 00:00 [pmc-release]
AID - genes9020089 [pii]
AID - genes-09-00089 [pii]
AID - 10.3390/genes9020089 [doi]
PST - epublish
SO  - Genes (Basel). 2018 Feb 14;9(2):89. doi: 10.3390/genes9020089.