PMID- 29445895
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240314
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 70
IP  - 2
DP  - 2018 Apr
TI  - On reprogramming of tumor cells metabolism: detection of glycogen in the cell 
      lines of hepatocellular origin with various degrees of dedifferentiation.
PG  - 879-890
LID - 10.1007/s10616-018-0200-1 [doi]
AB  - The reprogramming of cancer cells includes shifts in glucose and glycogen 
      metabolism. The aim of our work was to check the ability of forming glycogen 
      grains in hepatocellular tumor cell lines of various dedifferentiation levels. We 
      studied the monolayer culture established in vitro after explanting cells from 
      rat ascites Zajdela hepatoma strain C (ZH-C) as a "parental" line and its five 
      daughter clonal sublines: the holoclonal sublines 3H, 5F, 6H and the meroclonal 
      ones 1E, 9C, which possess, respectively, the properties of cancer stem-like 
      cells (CSLCs) and cancer progenitor-like cells (CPLCs). Besides, we studied four 
      permanent cell lines of a rat hepatoma HTC, two murine hepatomas BWTG3 and 
      MH-22a, and human hepatoblastoma HepG2. We used normal rat hepatocytes as 
      positive control cells that form glycogen. We estimated relative cell 
      dedifferentiation levels of the studied lines via analysis of cell morphology, 
      morphometry and motility character on stained cell preparations and lifetime 
      video files. Glycogen in the cells was detected using a Schiff type Au-SO(2) 
      reagent. All studied hepatocellular tumor lines were not of equal 
      dedifferentiation level as manifested by different nucleus-to-cytoplasm ratio, by 
      epithelium-like or fibroblast-like morphology, by tight or loosen intercellular 
      contacts, by cell migration of collective or individual types. Glycogen 
      fluorescence of uneven intensity was observed in all normal rat hepatocytes, but 
      only in some cell groups or in single cells of hepatocellular tumor lines. The 
      large or small fluorescent grains were found not only in relatively less 
      dedifferentiated parental ZH-C line, BWTG3 and HepG2 lines, but also in 
      moderately dedifferentiated 1E and HTC lines and even in severely 
      dedifferentiated 3H, 5F and 6H sublines, as well as in the islets of the rat 
      ascites hepatoma induced in vivo by the injection of 3H cells (the 
      tumor-initiating cells). On the other hand, MH-22 and 9C lines, being relatively 
      less and moderately dedifferentiated, showed no glycogen fluorescence. Thus, in 
      10 tumor cell lines of hepatocellular origin, an ability to reserve glycogen 
      manifested no obvious dependency on their dedifferentiation level. Glycogen 
      grains were detected in some cells even of the severely dedifferentiated lines: 
      in single CSLCs of holoclonal ZH sublines grown in vitro and in a majority of 
      tumor-initiating cells derived from ascites hepatoma in vivo. We suggest that 
      dynamic changes in glycogen formation in CSLCs and tumor-initiating cells might 
      be of importance for their dedifferentiation, self-renewal in vitro, survival and 
      metastasis in vivo. The role of glycogen in maintaining viability and metastasis 
      of tumor cells is to be further studied.
FAU - Teryukova, Natalya P
AU  - Teryukova NP
AD  - Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, Saint 
      Petersburg, Russia, 194064.
FAU - Malkova, Victoria V
AU  - Malkova VV
AD  - Herzen State Pedagogical University of Russia, Saint Petersburg, Russia.
FAU - Sakhenberg, Elena I
AU  - Sakhenberg EI
AD  - Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, Saint 
      Petersburg, Russia, 194064.
FAU - Ivanov, Vadim A
AU  - Ivanov VA
AD  - Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, Saint 
      Petersburg, Russia, 194064.
FAU - Bezborodkina, Natalia N
AU  - Bezborodkina NN
AD  - Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, Saint 
      Petersburg, Russia, 194064.
FAU - Snopov, Sergei A
AU  - Snopov SA
AUID- ORCID: 0000-0002-4423-6125
AD  - Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, Saint 
      Petersburg, Russia, 194064. snopov@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20180214
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC5851979
OTO - NOTNLM
OT  - Cancer stem-like cells
OT  - Glycogen
OT  - Hepatocellular tumor cell lines
OT  - Hepatoma Zajdela
OT  - Tumor reprogramming
OT  - Types of cell migration
COIS- The animal experiments were conducted in conformity with international and 
      Russian laws and policies CONFLICT OF INTEREST: The authors declare that they 
      have no conflict of interest.
EDAT- 2018/02/16 06:00
MHDA- 2018/02/16 06:01
PMCR- 2019/04/01
CRDT- 2018/02/16 06:00
PHST- 2017/09/28 00:00 [received]
PHST- 2018/01/24 00:00 [accepted]
PHST- 2018/02/16 06:00 [pubmed]
PHST- 2018/02/16 06:01 [medline]
PHST- 2018/02/16 06:00 [entrez]
PHST- 2019/04/01 00:00 [pmc-release]
AID - 10.1007/s10616-018-0200-1 [pii]
AID - 200 [pii]
AID - 10.1007/s10616-018-0200-1 [doi]
PST - ppublish
SO  - Cytotechnology. 2018 Apr;70(2):879-890. doi: 10.1007/s10616-018-0200-1. Epub 2018 
      Feb 14.