PMID- 2944845
OWN - NLM
STAT- MEDLINE
DCOM- 19861107
LR  - 20190816
IS  - 0020-5915 (Print)
IS  - 0020-5915 (Linking)
VI  - 81
IP  - 2
DP  - 1986
TI  - Feedback regulation of antibody formation by suppressive B cell factor: 
      preferential suppression of high-affinity antibody production by memory B 
      lymphocytes.
PG  - 156-64
AB  - Cloned TS4.44 cells, which were hybridized HAT-sensitive 3T3-4E cells with B 
      cells stimulated by immune complexes produce a lymphokine, biochemical and 
      biological characteristics of which are identical with those of conventional 
      suppressive B cell factor (SBF) synthesized by Fc receptor bearing B cells 
      stimulated with immune complexes. This factor is known to suppress B cell 
      responses to antigen/mitogen. The present studies were carried out by using this 
      hybridoma-derived SBF to characterize the large proportion of B cells sensitive 
      to SBF and the small proportion of B cells resistant to it in terms of affinities 
      of antibodies which these cells are able to produce. The treatment of normal 
      spleen cells with SBF resulted in a 50-70% decrease in anti-dinitrophenyl (DNP) 
      antibody production when the cells were transferred into X-ray-irradiated mice 
      along with alum-precipitated dinitrophenyl-conjugated keyhole limpet hemocyanin 
      (DNP-KLH). The affinity of anti-DNP antibody molecules produced in these mice was 
      significantly lower than that of the controls, even if immunization was repeated. 
      The target cells for SBF were B, and not helper T cells which might be involved 
      in the process of affinity maturation. A single treatment of spleen cells in 
      vitro with SBF was sufficient to abrogate the precursors committed to mediate 
      high-affinity anti-DNP antibody responses, since the retreatment with SBF in 
      vitro and transfer into the second irradiated recipients along with antigens of 
      spleen cells of mice to which SBF-treated spleen cells were transferred 3 weeks 
      before resulted in almost the same level of plaque-forming-cell-responses as in 
      mice which received medium-treated.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Park, Y H
AU  - Park YH
FAU - Suzuki, T
AU  - Suzuki T
FAU - Miyama-Inaba, M
AU  - Miyama-Inaba M
FAU - Masuda, T
AU  - Masuda T
FAU - Yoshida, Y
AU  - Yoshida Y
FAU - Uchino, H
AU  - Uchino H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Int Arch Allergy Appl Immunol
JT  - International archives of allergy and applied immunology
JID - 0404561
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Suppressor Factors, Immunologic)
SB  - IM
MH  - Animals
MH  - Antibody Affinity
MH  - *Antibody Formation
MH  - B-Lymphocytes/cytology/*immunology
MH  - Cell Differentiation
MH  - Dinitrobenzenes/immunology
MH  - Feedback
MH  - Female
MH  - Hybridomas
MH  - Immunization, Passive
MH  - *Immunologic Memory
MH  - Male
MH  - Mice
MH  - Spleen/cytology
MH  - Suppressor Factors, Immunologic/*immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
AID - 10.1159/000234125 [doi]
PST - ppublish
SO  - Int Arch Allergy Appl Immunol. 1986;81(2):156-64. doi: 10.1159/000234125.