PMID- 29449560
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 2041-4889 (Electronic)
VI  - 9
IP  - 3
DP  - 2018 Feb 15
TI  - Knockout of zebrafish interleukin 7 receptor (IL7R) by the CRISPR/Cas9 system 
      delays retinal neurodevelopment.
PG  - 273
LID - 10.1038/s41419-018-0337-z [doi]
LID - 273
AB  - Interleukin 7 receptor (il7r), a transmembrane receptor, belongs to the type I 
      cytokine receptor family. Il7r is involved in the pathogenesis of 
      neurodegenerative disorders, such as multiple sclerosis. Targeted knockdown of 
      il7r leads to delayed myelination, highlighting the potential role of il7r in the 
      development of the nervous system. Zebrafish is an ideal model for the study of 
      neurogenesis; moreover, the il7r gene is highly conserved between zebrafish and 
      human. The aim of the present study was to investigate the novel function of il7r 
      in neurogenesis. First, an il7r (-/-) homozygous mutant line was generated by 
      clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 
      (CRISPR/Cas9) technology. Second, the gross development of il7r(-/-) mutants 
      revealed remarkably smaller eyes and delayed retinal neurodifferentiation. Third, 
      microarray analysis revealed that genes associated with the phototransduction 
      signalling pathway were strongly down-regulated in il7r (-/-) mutants. Finally, 
      the results from behavioural tests indicated that visual function was impaired in 
      il7r (-/-) mutant larvae. Overall, our data demonstrate that a lack of il7r 
      retards the development of the retina. Thus, il7r is an essential molecule for 
      maintaining normal retinal development in zebrafish.
FAU - Cai, Shijiao
AU  - Cai S
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin, 300071, China.
AD  - State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, 
      Nankai University, Tianjin, 300350, China.
FAU - Chen, Yang
AU  - Chen Y
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin, 300071, China.
FAU - Shang, Yue
AU  - Shang Y
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin, 300071, China.
FAU - Cui, Jianlin
AU  - Cui J
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin, 300071, China.
AD  - Medical International Collaborative Innovation Center, Nankai University, 
      Tianjin, 300071, China.
FAU - Li, Zongjin
AU  - Li Z
AUID- ORCID: 0000-0002-4603-3743
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin, 300071, China.
FAU - Li, Yuhao
AU  - Li Y
AUID- ORCID: 0000-0003-2022-9526
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin, 300071, China. liyuhao@nankai.edu.cn.
AD  - Medical International Collaborative Innovation Center, Nankai University, 
      Tianjin, 300071, China. liyuhao@nankai.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180215
PL  - England
TA  - Cell Death Dis
JT  - Cell death & disease
JID - 101524092
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (Zebrafish Proteins)
RN  - EC 3.1.- (CRISPR-Associated Protein 9)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - CRISPR-Associated Protein 9/*genetics/metabolism
MH  - *CRISPR-Cas Systems
MH  - *Clustered Regularly Interspaced Short Palindromic Repeats
MH  - Gene Expression Regulation, Developmental
MH  - *Gene Knockdown Techniques
MH  - Mutation
MH  - *Neurogenesis
MH  - Photic Stimulation
MH  - Receptors, Interleukin-7/*deficiency/genetics
MH  - Retinal Neurons/*metabolism
MH  - Signal Transduction
MH  - Vision, Ocular
MH  - Zebrafish/embryology/genetics/*metabolism
MH  - Zebrafish Proteins/*deficiency/genetics
PMC - PMC5833684
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/02/17 06:00
MHDA- 2020/04/09 06:00
PMCR- 2018/02/15
CRDT- 2018/02/17 06:00
PHST- 2017/10/31 00:00 [received]
PHST- 2018/01/22 00:00 [accepted]
PHST- 2018/01/04 00:00 [revised]
PHST- 2018/02/17 06:00 [entrez]
PHST- 2018/02/17 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2018/02/15 00:00 [pmc-release]
AID - 10.1038/s41419-018-0337-z [pii]
AID - 337 [pii]
AID - 10.1038/s41419-018-0337-z [doi]
PST - epublish
SO  - Cell Death Dis. 2018 Feb 15;9(3):273. doi: 10.1038/s41419-018-0337-z.