PMID- 29449832
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Rhizosphere Competence and Biocontrol Effect of Pseudomonas sp. RU47 Independent 
      from Plant Species and Soil Type at the Field Scale.
PG  - 97
LID - 10.3389/fmicb.2018.00097 [doi]
LID - 97
AB  - Biocontrol inoculants often show inconsistency in their efficacy at field scale 
      and the reason for this remains often unclear. A high rhizosphere competence of 
      inoculant strains is assumed to be a key factor for successful biocontrol effects 
      as the biocontrol strain has to compete with the indigenous microbial community 
      in the rhizosphere. It is known that many factors, among them plant species and 
      soil type shape the rhizosphere microbial community composition. However, 
      microbial community composition in the rhizosphere can also be influenced by the 
      presence of a pathogen. We hypothesized that plant species, soil type, and a 
      pathogen affect the rhizosphere competence of a biocontrol strain and its 
      biocontrol effect against a soil-borne pathogen. To test the hypothesis, we used 
      an experimental plot system with three soil types (diluvial sand, alluvial loam, 
      loess loam) kept under similar agricultural management at the same field site for 
      12 years. We investigate the rhizosphere competence of Pseudomonas sp. RU47 in 
      two plant species (potato and lettuce) and its biocontrol effect against 
      Rhizoctonia diseases. The colonization density of a rifampicin resistant mutant 
      of RU47 in the rhizosphere of both crops was evaluated by plate counts. Bacterial 
      community compositions were analyzed by denaturing gradient gel electrophoresis 
      (DGGE) of 16S rRNA gene fragments amplified from total community DNA. The 
      inoculant RU47 was able to colonize the rhizosphere of both model crops in a 
      sufficient density and to reduce disease severity of black scurf on potato and 
      bottom rot on lettuce in all three soils. DGGE indicated that RU47 affected the 
      bacterial community composition stronger in the rhizosphere of lettuce than in 
      the potato rhizosphere. In contrast, the effect of the pathogen Rhizoctonia 
      solani on the bacterial community was much stronger in the rhizosphere of potato 
      than in the lettuce rhizosphere. A significant effect of RU47 on the 
      Pseudomonas-specific gacA fingerprints of the rhizosphere was only observed in 
      lettuce in alluvial soil. The soil type and plant species independent biocontrol 
      effects of RU47 and its minor influence on the indigenous bacterial community 
      composition might be important criteria for the registration and use of RU47 as 
      biocontrol strain.
FAU - Schreiter, Susanne
AU  - Schreiter S
AD  - Institute for Epidemiology and Pathogen Diagnostics, Federal Research Centre for 
      Cultivated Plants, Julius Kuhn-Institut (JKI), Braunschweig, Germany.
FAU - Babin, Doreen
AU  - Babin D
AD  - Institute for Epidemiology and Pathogen Diagnostics, Federal Research Centre for 
      Cultivated Plants, Julius Kuhn-Institut (JKI), Braunschweig, Germany.
FAU - Smalla, Kornelia
AU  - Smalla K
AD  - Institute for Epidemiology and Pathogen Diagnostics, Federal Research Centre for 
      Cultivated Plants, Julius Kuhn-Institut (JKI), Braunschweig, Germany.
FAU - Grosch, Rita
AU  - Grosch R
AD  - Department Plant-Microbe Systems, Leibniz Institute of Vegetable and Ornamental 
      Crops, Grossbeeren, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180201
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5799239
OTO - NOTNLM
OT  - DGGE
OT  - Rhizoctonia solani
OT  - bacterial community
OT  - biocontrol
OT  - plant disease
OT  - plant health
EDAT- 2018/02/17 06:00
MHDA- 2018/02/17 06:01
PMCR- 2018/02/01
CRDT- 2018/02/17 06:00
PHST- 2017/10/24 00:00 [received]
PHST- 2018/01/16 00:00 [accepted]
PHST- 2018/02/17 06:00 [entrez]
PHST- 2018/02/17 06:00 [pubmed]
PHST- 2018/02/17 06:01 [medline]
PHST- 2018/02/01 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.00097 [doi]
PST - epublish
SO  - Front Microbiol. 2018 Feb 1;9:97. doi: 10.3389/fmicb.2018.00097. eCollection 
      2018.