PMID- 29452235
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20180920
IS  - 0304-4165 (Print)
IS  - 0304-4165 (Linking)
VI  - 1862
IP  - 5
DP  - 2018 May
TI  - Improved strength of silk fibers in Bombyx mori trimolters induced by an 
      anti-juvenile hormone compound.
PG  - 1148-1156
LID - S0304-4165(18)30049-7 [pii]
LID - 10.1016/j.bbagen.2018.02.007 [doi]
AB  - BACKGROUND: Bombyx mori silk fibers with thin diameters have advantages of 
      lightness and crease-resistance. Many studies have used anti-juvenile hormones to 
      induce trimolters in order to generate thin silk; however, there has been 
      comparatively little analysis of the morphology, structure and mechanical 
      properties of trimolter silk. METHODS: This study induced two kinds of trimolters 
      by appling topically anti-juvenile hormones and obtained thin diameter silk. 
      Scanning electron microscope (SEM), FTIR analysis, tensile mechanical testing, 
      chitin staining were used to reveal that the morphology, conformation and 
      mechanical property of the trimolter silk. RESULTS: Cocoon of trimolters were 
      highly densely packed by thinner fibers and thus had small apertures. We found 
      that the conformation of trimolter silk fibroin changed and formed more beta-sheet 
      structures. In addition, analysis of mechanical parameters yielded a higher 
      Young's modulus and strength in trimolter silk than in the control. By chitin 
      staining of silk gland, we postulated that the mechanical properties of 
      trimolters' silk was enhanced greatly during to the structural changes of silk 
      gland. CONCLUSION: We induced trimolters by anti-juvenile hormones and the 
      resulting cocoons were more closely packed and had smaller silk fiber diameters. 
      We found that the conformation of trimolters silk fibroin had a higher content of 
      beta-sheet structures and better mechanical properties. GENERAL SIGNIFICANCE: Our 
      study revealed the structures and mechanical properties of trimolter silk, and 
      provided a valuable reference to improve silk quality by influencing molting in 
      silkworms.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Guo, Kaiyu
AU  - Guo K
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Dong, Zhaoming
AU  - Dong Z
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China; Chongqing Engineering and Technology Research Center for Novel 
      Silk Materials, Chongqing 400716, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China; Chongqing Engineering and Technology Research Center for Novel 
      Silk Materials, Chongqing 400716, China; College of Biotechnology, Southwest 
      University, Chongqing 400716, China.
FAU - Wang, Dandan
AU  - Wang D
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Tang, Muya
AU  - Tang M
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Zhang, Xiaolu
AU  - Zhang X
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Xia, Qingyou
AU  - Xia Q
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Zhao, Ping
AU  - Zhao P
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China. Electronic address: zhaop@swu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180213
PL  - Netherlands
TA  - Biochim Biophys Acta Gen Subj
JT  - Biochimica et biophysica acta. General subjects
JID - 101731726
RN  - 0 (Imidazoles)
RN  - 0 (Juvenile Hormones)
RN  - 0 (Silk)
RN  - 96108-89-3 (1-benzyl-5-(2,6-dimethyl-1,5-heptadienyl)imidazole)
SB  - IM
MH  - Animals
MH  - Bombyx/chemistry/*metabolism
MH  - Elastic Modulus
MH  - Imidazoles/*pharmacology
MH  - Juvenile Hormones/*antagonists & inhibitors/metabolism/pharmacology
MH  - Silk/*biosynthesis/*chemistry
OTO - NOTNLM
OT  - Anti-juvenile hormone
OT  - Mechanical property
OT  - Silk fiber
OT  - Trimolter
EDAT- 2018/02/17 06:00
MHDA- 2018/07/10 06:00
CRDT- 2018/02/17 06:00
PHST- 2017/08/24 00:00 [received]
PHST- 2017/12/21 00:00 [revised]
PHST- 2018/02/12 00:00 [accepted]
PHST- 2018/02/17 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
PHST- 2018/02/17 06:00 [entrez]
AID - S0304-4165(18)30049-7 [pii]
AID - 10.1016/j.bbagen.2018.02.007 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Gen Subj. 2018 May;1862(5):1148-1156. doi: 
      10.1016/j.bbagen.2018.02.007. Epub 2018 Feb 13.