PMID- 29455108
OWN - NLM
STAT- MEDLINE
DCOM- 20190429
LR  - 20190429
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 105
DP  - 2018 May
TI  - Ureaplasma isolates differentially modulate growth factors and cell adhesion 
      molecules in human neonatal and adult monocytes.
PG  - 45-48
LID - S1043-4666(18)30039-5 [pii]
LID - 10.1016/j.cyto.2018.01.026 [doi]
AB  - Generally regarded as commensal bacteria, the pathogenicity of Ureaplasma has 
      often been considered low. Controversy remains concerning the clinical relevance 
      of Ureaplasma infection in the pathogenesis of inflammation-related morbidities. 
      Recently, we demonstrated Ureaplasma-driven pro-inflammatory cytokine responses 
      in human monocytes in vitro. We hypothesized that Ureaplasma may induce further 
      inflammatory mediators. Using qRT-PCR and multi-analyte immunoassay, we assessed 
      the expression of granulocyte-colony stimulating factor (G-CSF), vascular 
      endothelial growth factor (VEGF), intercellular adhesion molecule 1 (ICAM-1) and 
      vascular cell adhesion molecule 1 (VCAM-1) in term neonatal and adult monocytes 
      exposed to Ureaplasma urealyticum serovar 8 (Uu8) and U. parvum serovar 3 (Up3). 
      Ureaplasma significantly induced VEGF mRNA in neonatal (Up3: p < 0.05, versus 
      broth control) and adult monocytes (Uu8: p < 0.05) as well as ICAM-1 mRNA in 
      neonatal cells (p < 0.05 each). As far as protein expression was concerned, Up3 
      stimulated VEGF release in both monocyte subsets (p < 0.01) and enhanced 
      secretion of ICAM-1 protein in neonatal monocytes (p < 0.05). In adult cells, 
      ICAM-1 protein release was increased upon exposure to both isolates (Uu8: 
      p < 0.05, Up3: p < 0.01). Ureaplasma-induced responses did not significantly 
      differ from corresponding levels mediated by E. coli lipopolysaccharide (LPS). 
      The stimulatory effects were dose-dependent. Ureaplasma infection, on the 
      contrary, did not affect G-CSF and VCAM-1 expression. Of note, co-infection of 
      LPS-primed neonatal monocytes with Ureaplasma enhanced LPS-induced ICAM-1 release 
      (Uu8: p < 0.05). Our results confirm Ureaplasma-driven pro-inflammatory 
      activation of human monocytes in vitro, demonstrating a differential modulation 
      of growth factors and cell adhesion molecules, that might promote unbalanced 
      monocyte responses and adverse immunomodulation.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Glaser, Kirsten
AU  - Glaser K
AD  - University Children's Hospital, University of Wuerzburg, Wuerzburg, Germany. 
      Electronic address: Glaser_K@ukw.de.
FAU - Silwedel, Christine
AU  - Silwedel C
AD  - University Children's Hospital, University of Wuerzburg, Wuerzburg, Germany.
FAU - Waaga-Gasser, Ana Maria
AU  - Waaga-Gasser AM
AD  - Department of Surgery I, Molecular Oncology and Immunology, University of 
      Wuerzburg, Wuerzburg, Germany.
FAU - Henrich, Birgit
AU  - Henrich B
AD  - Institute of Medical Microbiology and Hospital Hygiene, University Clinic of 
      Heinrich-Heine University Duesseldorf, Duesseldorf, Germany.
FAU - Fehrholz, Markus
AU  - Fehrholz M
AD  - University Children's Hospital, University of Wuerzburg, Wuerzburg, Germany.
FAU - Claus, Heike
AU  - Claus H
AD  - Institute for Hygiene and Microbiology, University of Wuerzburg, Wuerzburg, 
      Germany.
FAU - Speer, Christian P
AU  - Speer CP
AD  - University Children's Hospital, University of Wuerzburg, Wuerzburg, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180216
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - Intercellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/drug effects/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Ureaplasma/*isolation & purification/*physiology
OTO - NOTNLM
OT  - Cell adhesion molecules
OT  - G-CSF
OT  - Growth factors
OT  - ICAM-1
OT  - Inflammation
OT  - Monocytes
OT  - Ureaplasma
OT  - VCAM-1
OT  - VEGF
EDAT- 2018/02/20 06:00
MHDA- 2019/04/30 06:00
CRDT- 2018/02/19 06:00
PHST- 2017/12/19 00:00 [received]
PHST- 2018/01/29 00:00 [revised]
PHST- 2018/01/30 00:00 [accepted]
PHST- 2018/02/20 06:00 [pubmed]
PHST- 2019/04/30 06:00 [medline]
PHST- 2018/02/19 06:00 [entrez]
AID - S1043-4666(18)30039-5 [pii]
AID - 10.1016/j.cyto.2018.01.026 [doi]
PST - ppublish
SO  - Cytokine. 2018 May;105:45-48. doi: 10.1016/j.cyto.2018.01.026. Epub 2018 Feb 16.