PMID- 29455975 OWN - NLM STAT- MEDLINE DCOM- 20180910 LR - 20180910 IS - 1879-1344 (Electronic) IS - 0144-8617 (Linking) VI - 186 DP - 2018 Apr 15 TI - Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation. PG - 17-24 LID - S0144-8617(18)30036-5 [pii] LID - 10.1016/j.carbpol.2018.01.036 [doi] AB - A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study. Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system. RPM could flocculate marine microalgae Chlorella salina with a FR of 74.1%. The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content. The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively. It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation. CI - Copyright (c) 2018 Elsevier Ltd. All rights reserved. FAU - Mu, Jun AU - Mu J AD - School of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: mujun@zjou.edu.cn. FAU - Zhou, Haijun AU - Zhou H AD - School of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: 1147912513@qq.com. FAU - Chen, Yin AU - Chen Y AD - School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: mojojo1984@163.com. FAU - Yang, Guangfeng AU - Yang G AD - School of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: 514972297@qq.com. FAU - Cui, Xia AU - Cui X AD - School of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: 785776569@qq.com. LA - eng PT - Journal Article DEP - 20180111 PL - England TA - Carbohydr Polym JT - Carbohydrate polymers JID - 8307156 RN - 0 (Polysaccharides) SB - IM MH - Animals MH - Bivalvia/chemistry MH - Chlorella/chemistry MH - Flocculation MH - Microalgae/chemistry MH - Polysaccharides/*chemistry OTO - NOTNLM OT - Bioflocculant OT - Marine microalgae flocculation OT - Polysaccharides OT - Ruditapes philippinarum conglutination mud OT - Synergistic flocculation EDAT- 2018/02/20 06:00 MHDA- 2018/09/11 06:00 CRDT- 2018/02/20 06:00 PHST- 2017/08/22 00:00 [received] PHST- 2017/12/19 00:00 [revised] PHST- 2018/01/10 00:00 [accepted] PHST- 2018/02/20 06:00 [entrez] PHST- 2018/02/20 06:00 [pubmed] PHST- 2018/09/11 06:00 [medline] AID - S0144-8617(18)30036-5 [pii] AID - 10.1016/j.carbpol.2018.01.036 [doi] PST - ppublish SO - Carbohydr Polym. 2018 Apr 15;186:17-24. doi: 10.1016/j.carbpol.2018.01.036. Epub 2018 Jan 11.