PMID- 29456655
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 15
IP  - 3
DP  - 2018 Mar
TI  - Effect of immune tolerance induced by immature dendritic cells and CTLA4-Ig on 
      systemic lupus erythematosus: An in vivo study.
PG  - 2499-2506
LID - 10.3892/etm.2018.5697 [doi]
AB  - Systemic lupus erythematosus (SLE) is a multi-organ autoimmune disease in which 
      tissue damage is caused by autoantibodies. The induction of specific immune 
      tolerance, including the utilization of immune regulatory cells, may enhance the 
      therapeutic effects of organ transplantation in patients with SLE. Furthermore, 
      inhibiting immune responses has been reported to be an effective treatment for 
      SLE. However, few studies have explored the association between an increased 
      immune tolerance and a decreased immune response in SLE treatment. Dendritic 
      cells (DCs), which are highly efficient antigen-presenting cells, are able to 
      induce specific tolerance, while cytotoxic T lymphocyte antigen 4-immunoglobulin 
      (CTLA4-Ig) inhibits the immune response. In the present study, interleukin 
      (IL)-10-treated DCs and CTLA4-Ig were administered to mice with SLE alone or in 
      combination and the therapeutic effects were investigated. IL-10 was added into 
      the culture medium of bone marrow-derived DCs to prevent them from 
      differentiating into mature cells. Low levels of major histocompatibility complex 
      II, cluster of differentiation (CD)40, CD80 and CD86 were detected, which 
      indicated that the immature state of DCs was maintained. IL-10-treated DCs were 
      subsequently injected into the caudal vein of B6.MRL-Fas(lpr)/J lupus mice, which 
      are an established animal model of SLE. To amplify the tolerance effect, mice 
      were simultaneously injected with CTLA4-Ig. Compared with the IL-10-treated DC 
      and CTLA4-Ig groups, combined treatment with IL-10-treated DCs and CTLA4-Ig 
      strongly induced immune tolerance in mice with SLE, as indicated by the 
      significantly reduced levels of urine protein, anti-nuclear antibody, 
      double-stranded DNA and IL-17A. A significant decrease in the proportion of T 
      helper cells and an increase in the proportion of CD4(+) forkhead box protein 
      P3(+) Treg cells was also observed, further confirming the induction of immune 
      tolerance. These results suggest that combined treatment with IL-10-DCs and 
      CTLA4-Ig may be a promising novel therapeutic strategy for the treatment of SLE.
FAU - Huang, Cuili
AU  - Huang C
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Zhang, Lidan
AU  - Zhang L
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Ling, Fang
AU  - Ling F
AD  - Department of Cell Biology and Genetics, Guangxi Medical University, Nanning, 
      Guangxi 530021, P.R. China.
FAU - Wen, Sijian
AU  - Wen S
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Luo, Yanyan
AU  - Luo Y
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Liu, Hui
AU  - Liu H
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Liu, Jingping
AU  - Liu J
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Zheng, Wenjun
AU  - Zheng W
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Liang, Ming
AU  - Liang M
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
FAU - Sun, Jian
AU  - Sun J
AD  - Department of Cell Biology and Genetics, Guangxi Medical University, Nanning, 
      Guangxi 530021, P.R. China.
FAU - Lin, You-Kun
AU  - Lin YK
AD  - Department of Dermatology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi 530021, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180104
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5795536
OTO - NOTNLM
OT  - cytotoxic T lymphocyte antigen 4-immunoglobulin
OT  - immature dendritic cells
OT  - interleukin-10
OT  - systemic lupus erythematosus
OT  - tolerance
EDAT- 2018/02/20 06:00
MHDA- 2018/02/20 06:01
PMCR- 2018/01/04
CRDT- 2018/02/20 06:00
PHST- 2016/09/21 00:00 [received]
PHST- 2017/07/27 00:00 [accepted]
PHST- 2018/02/20 06:00 [entrez]
PHST- 2018/02/20 06:00 [pubmed]
PHST- 2018/02/20 06:01 [medline]
PHST- 2018/01/04 00:00 [pmc-release]
AID - ETM-0-0-5697 [pii]
AID - 10.3892/etm.2018.5697 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Mar;15(3):2499-2506. doi: 10.3892/etm.2018.5697. Epub 2018 Jan 
      4.