PMID- 29457605
OWN - NLM
STAT- MEDLINE
DCOM- 20180817
LR  - 20190606
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 24
DP  - 2018 Feb 19
TI  - Negative Pressure Therapy in the Regeneration of the Sciatic Nerve Using Vacuum - 
      Assisted Closure in a Rabbit Model.
PG  - 1027-1033
AB  - BACKGROUND The aim of this study was to investigate the effects of negative 
      pressure therapy in the regeneration of the rabbit sciatic nerve using vacuum 
      assisted closure (VAC). MATERIAL AND METHODS Thirty male New Zealand white 
      rabbits underwent surgical injury of the sciatic nerve, followed by negative 
      pressure therapy using vacuum assisted closure (VAC), in three treatment groups: 
      Group A: 0 kPa; Group B: -20 kPa; Group C: -40 kPa. At 12 weeks following 
      surgery, the following factors were studied: motor nerve conduction velocity 
      (MNCV); the number of myelinated nerve fibers; the wet weight of the 
      gastrocnemius muscle. Gastrocnemius muscle and sciatic nerve tissue samples were 
      studied for the expression of S100, and brain-derived neurotrophic factor (BDNF) 
      using Western blot. RESULTS At 12 weeks following VAC treatment, the MNCV, number 
      of myelinated nerve fibers, and wet weight of the gastrocnemius muscle showed 
      significant differences between the groups (p<0.05), in the following order: 
      Group B >Group A >Group C. The sciatic nerve at 12 weeks following VAC in Group B 
      and Group C showed a significant increase in expression of S100 and BDNF when 
      compared with Group A; no significant differences were detected between Group B 
      and Group C results from Western blot at 12 weeks. CONCLUSIONS The findings of 
      this study, using negative pressure therapy in VAC in a rabbit model of sciatic 
      nerve damage, have shown that moderate negative pressure was beneficial, but high 
      values did not benefit sciatic nerve repair.
FAU - Wu, Jian-Ping
AU  - Wu JP
AD  - Department of Orthopaedics, Yixing Hospital of Jiangsu Unversity, Yixin, Jiangsu, 
      China (mainland).
FAU - Jiang, Zhen-Huan
AU  - Jiang ZH
AD  - Department of Orthopaedics, Yixing Hospital of Jiangsu Unversity, Yixin, Jiangsu, 
      China (mainland).
FAU - Feng, Xiao-Jun
AU  - Feng XJ
AD  - Xishan People's Hospital of Wuxi, Wuxing, Jiangsu, China (mainland).
FAU - Jiang, Jian-Nong
AU  - Jiang JN
AD  - Department of Orthopaedics, Yixing Hospital of Jiangsu Unversity, Yixin, Jiangsu, 
      China (mainland).
FAU - Cheng, Mao-Hua
AU  - Cheng MH
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Soochow, Suzhou, 
      Jiangsu, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20180219
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (S100 Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Disease Models, Animal
MH  - Male
MH  - Motor Neurons/pathology
MH  - Muscle, Skeletal/pathology
MH  - Myelin Sheath/metabolism
MH  - *Negative-Pressure Wound Therapy
MH  - Nerve Fibers/pathology
MH  - *Nerve Regeneration
MH  - Neural Conduction
MH  - Organ Size
MH  - Rabbits
MH  - S100 Proteins/metabolism
MH  - Sciatic Nerve/pathology/*physiopathology
PMC - PMC5827701
COIS- Competing interests None.
EDAT- 2018/02/20 06:00
MHDA- 2018/08/18 06:00
PMCR- 2018/02/19
CRDT- 2018/02/20 06:00
PHST- 2018/02/20 06:00 [entrez]
PHST- 2018/02/20 06:00 [pubmed]
PHST- 2018/08/18 06:00 [medline]
PHST- 2018/02/19 00:00 [pmc-release]
AID - 906696 [pii]
AID - 10.12659/msm.906696 [doi]
PST - epublish
SO  - Med Sci Monit. 2018 Feb 19;24:1027-1033. doi: 10.12659/msm.906696.