PMID- 29457614
OWN - NLM
STAT- MEDLINE
DCOM- 20181224
LR  - 20190116
IS  - 1473-2262 (Electronic)
IS  - 1473-2262 (Linking)
VI  - 35
DP  - 2018 Feb 19
TI  - Poly(trimethylene carbonate) and poly(D,L-lactic acid) modify human dendritic 
      cell responses to staphylococci but do not affect Th1 and Th2 cell development.
PG  - 103-116
LID - 10.22203/eCM.v035a08 [doi]
AB  - Biomaterial-associated infections (BAIs) are frequent complications in the use of 
      medical devices (biomaterials) correlated with considerable patient discomfort 
      and high treatment costs. The presence of a biomaterial in the host causes 
      derangement of local immune responses increasing susceptibility to infection. 
      Dendritic cells (DCs) have an important role in directing the nature of immune 
      responses by activating and controlling CD4+ T helper (Th) cell responses. To 
      assess the immunomodulatory effect of the combined presence of biomaterials and 
      Staphylococcus aureus (S. aureus) or Staphylococcus epidermidis (S. epidermidis), 
      DC-mediated T cell proliferation and Th1/Th2 cell development were measured using 
      an in vitro human cell system. Poly(trimethylene carbonate) (PTMC) and 
      poly(D,L-lactic acid) (PDLLA) modified the production of the DC pro-inflammatory 
      cytokines TNF-alpha, IL-6 and IL-23 in response to S. aureus and S. epidermidis. 
      However, this modified cytokine production did not cause differences in Th1/Th2 
      cell polarisation, showing a Th1 cell predominance. In the absence of 
      staphylococci, neither of the biomaterials induced DC-mediated T cell 
      proliferation or Th1/Th2 cell polarisation. Moreover, either in the absence or 
      presence of the biomaterials, S. aureus was a more potent inducer of DC cytokine 
      secretion, T cell proliferation and Th1 cell development than S. epidermidis. In 
      conclusion, although PTMC and PDLLA modulated DC cytokine responses to 
      staphylococci, this did not alter the resulting Th cell development. This result 
      suggested that, in this human cell model, Th1/Th2 cell responses were mainly 
      determined by the species of bacteria and that PTMC or PDLLA did not detectably 
      influence these responses.
FAU - Balraadjsing, P P
AU  - Balraadjsing PP
FAU - de Jong, E C
AU  - de Jong EC
FAU - Grijpma, D W
AU  - Grijpma DW
FAU - Tanck, M W
AU  - Tanck MW
FAU - Zaat, S A
AU  - Zaat SA
AD  - Department of Medical Microbiology, Amsterdam Infection and Immunity Institute, 
      Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ, 
      Amsterdam, the Netherlands.s.a.zaat@amc.uva.nl.
LA  - eng
PT  - Journal Article
DEP - 20180219
PL  - United States
TA  - Eur Cell Mater
JT  - European cells & materials
JID - 100973416
RN  - 0 (Biocompatible Materials)
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Dioxanes)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 31852-84-3 (polytrimethylene carbonate)
RN  - 459TN2L5F5 (poly(lactide))
SB  - IM
MH  - Biocompatible Materials/pharmacology
MH  - Biomarkers/metabolism
MH  - Cell Polarity
MH  - Cell Proliferation/drug effects
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Dendritic Cells/drug effects/*microbiology
MH  - Dioxanes/*pharmacology
MH  - Humans
MH  - Polyesters/*pharmacology
MH  - Polymers/*pharmacology
MH  - Staphylococcus/*physiology
MH  - Th1 Cells/*cytology/drug effects
MH  - Th2 Cells/*cytology/drug effects
EDAT- 2018/02/20 06:00
MHDA- 2018/12/26 06:00
CRDT- 2018/02/20 06:00
PHST- 2018/02/20 06:00 [entrez]
PHST- 2018/02/20 06:00 [pubmed]
PHST- 2018/12/26 06:00 [medline]
AID - vol035a08 [pii]
AID - 10.22203/eCM.v035a08 [doi]
PST - epublish
SO  - Eur Cell Mater. 2018 Feb 19;35:103-116. doi: 10.22203/eCM.v035a08.