PMID- 29458098
OWN - NLM
STAT- MEDLINE
DCOM- 20190916
LR  - 20190916
IS  - 1095-9564 (Electronic)
IS  - 1074-7427 (Linking)
VI  - 149
DP  - 2018 Mar
TI  - Spermine protects from LPS-induced memory deficit via BDNF and TrkB activation.
PG  - 135-143
LID - S1074-7427(18)30032-7 [pii]
LID - 10.1016/j.nlm.2018.02.012 [doi]
AB  - Lipopolysaccharide (LPS) has been long known to promote neuroinflammation and 
      learning and memory deficits. Since spermine, one of the main natural polyamines 
      in the central nervous system, protects from LPS-induced memory deficit by a 
      mechanism that comprises GluN2B receptors, the aim of the present study was to 
      determine whether brain-derived neurotrophic factor (BDNF), tropomyosin-related 
      kinase B (TrkB) receptor and cAMP response element binding (CREB) are involved in 
      this protective effect of spermine. Adult male Swiss albino mice received, 
      immediately after training in the novel object recognition task, saline or LPS 
      (250 mug/kg, i.p.); 5 min later they received saline or spermine (0.3 mg/kg, i.p.) 
      and, when specified, 5 min thereafter saline or the TrkB receptor antagonist 
      ANA-12 (0.5 mg/kg, i.p.) in different flanks. Animals were tested 24 h after 
      training. Spermine protected from LPS-induced memory deficit and this protective 
      effect was reversed by ANA-12. In a subset of animals BDNF, CREB and phospho-CREB 
      immunoreactivity was determined in the hippocampi and cerebral cortex 4 h after 
      spermine injection. Spermine reversed the decrease of mature BDNF levels induced 
      by LPS in both hippocampus and cerebral cortex. Spermine increased phospho-CREB 
      content and phospho-CREB/total CREB ratio in the cerebral cortex of LPS-treated 
      mice. The results support that the protective effect of spermine on LPS-induced 
      memory deficits depends on TrkB receptor activation and is accompanied by 
      restoration of mature BDNF levels in hippocampus and cerebral cortex, as well as 
      increased CREB phosphorylation in the cerebral cortex.
CI  - Copyright (c) 2018. Published by Elsevier Inc.
FAU - Fruhauf-Perez, Pamella K
AU  - Fruhauf-Perez PK
AD  - Graduate Program in Pharmacology, Center of Health Sciences, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil.
FAU - Temp, Fernanda R
AU  - Temp FR
AD  - Graduate Program in Pharmacology, Center of Health Sciences, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil.
FAU - Pillat, Micheli M
AU  - Pillat MM
AD  - Biochemistry Department, Chemistry Institute, University of Sao Paulo, 05508-900, 
      Brazil.
FAU - Signor, Cristiane
AU  - Signor C
AD  - Graduate Program in Biological Sciences: Toxicological Biochemistry, Center of 
      Natural and Exact Sciences, Federal University of Santa Maria, RS, Brazil.
FAU - Wendel, Arithane Lorena
AU  - Wendel AL
AD  - Graduate Program in Biological Sciences: Toxicological Biochemistry, Center of 
      Natural and Exact Sciences, Federal University of Santa Maria, RS, Brazil.
FAU - Ulrich, Henning
AU  - Ulrich H
AD  - Biochemistry Department, Chemistry Institute, University of Sao Paulo, 05508-900, 
      Brazil.
FAU - Mello, Carlos F
AU  - Mello CF
AD  - Graduate Program in Pharmacology, Center of Health Sciences, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil. Electronic address: 
      cf.mello@smail.ufsm.br.
FAU - Rubin, Maribel A
AU  - Rubin MA
AD  - Graduate Program in Pharmacology, Center of Health Sciences, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil; Graduate Program in Biological Sciences: 
      Toxicological Biochemistry, Center of Natural and Exact Sciences, Federal 
      University of Santa Maria, RS, Brazil. Electronic address: marubin@smail.ufsm.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180216
PL  - United States
TA  - Neurobiol Learn Mem
JT  - Neurobiology of learning and memory
JID - 9508166
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neuroprotective Agents)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - EC 2.7.10.1 (Ntrk2 protein, mouse)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Hippocampus/*drug effects/metabolism
MH  - Lipopolysaccharides
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Memory Disorders/chemically induced/*metabolism
MH  - Mice
MH  - Neuroprotective Agents/*pharmacology
MH  - Phosphorylation/drug effects
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Signal Transduction/drug effects
MH  - Spermine/*pharmacology
OTO - NOTNLM
OT  - ANA-12
OT  - CREB
OT  - NMDA receptor
OT  - Neuroinflammation
OT  - Object recognition task
OT  - Polyamines
EDAT- 2018/02/20 06:00
MHDA- 2019/09/17 06:00
CRDT- 2018/02/20 06:00
PHST- 2017/08/30 00:00 [received]
PHST- 2018/01/18 00:00 [revised]
PHST- 2018/02/14 00:00 [accepted]
PHST- 2018/02/20 06:00 [pubmed]
PHST- 2019/09/17 06:00 [medline]
PHST- 2018/02/20 06:00 [entrez]
AID - S1074-7427(18)30032-7 [pii]
AID - 10.1016/j.nlm.2018.02.012 [doi]
PST - ppublish
SO  - Neurobiol Learn Mem. 2018 Mar;149:135-143. doi: 10.1016/j.nlm.2018.02.012. Epub 
      2018 Feb 16.