PMID- 29459528
OWN - NLM
STAT- MEDLINE
DCOM- 20190510
LR  - 20190510
IS  - 0021-4884 (Print)
IS  - 0021-4884 (Linking)
VI  - 67
IP  - 1
DP  - 2018
TI  - [A VALIDATION STUDY OF THE IMPROVED PRODUCT FOR MEASURING JAPANESE CYPRESS 
      POLLEN-SPECIFIC IgE (THERMO SCIENTIFIC() ImmunoCAP() ImmunoCAP JAPANESE CYPRESS 
      POLLEN-SPECIFIC IgE)].
PG  - 67-71
LID - 10.15036/arerugi.67.67 [doi]
AB  - BACKGROUND: Japanese cypress pollen is a major causative allergen of seasonal 
      allergic rhinitis in Japan. Although ImmunoCAP-specific immunoglobulin E (IgE) 
      reagent Japanese cypress pollen has been widely used as a diagnostic aid, its 
      sensitivity requires enhancement. This study evaluated an improved version of 
      this reagent. METHODS: Serum samples from 61 subjects who underwent Japanese 
      cypress pollen exposure testing in an environmental challenge chamber in Chiba 
      University were assessed using the conventional ImmunoCAPspecific IgE Japanese 
      cypress pollen product and the improved product. In addition, specific IgE for 
      Cha o 1 and Cha o 2, the primary allergen components of Japanese cypress pollen, 
      was evaluated and their reactivity to specific IgE was compared between the 
      conventional and improved products. RESULTS: The antibody titer of the improved 
      product was approximately 1.8-fold that of the conventional product. In addition, 
      higher correlations with Cha o 1 and Cha o 2 were observed for the improved 
      product than for the conventional product. The clinical sensitivity (>/=class 2) in 
      56 exposure test-positive subjects was better for the improved product (80.4%) 
      than for the conventional product (71.4%). CONCLUSIONS: An improvement of the 
      ImmunoCAP-specific IgE reagent Japanese cypress pollen resulted in enhanced 
      Japanese cypress pollen-specific IgE sensitivity. The primary reason for this 
      appeared to be an improved Cha o 1- and Cha o 2-specific IgE detectability.
FAU - Yonekura, Syuji
AU  - Yonekura S
AD  - Department of Otorhinolarngology, Head and Neck Surgery, Graduate School of 
      Medicine, Chiba University.
FAU - Okamoto, Yoshitaka
AU  - Okamoto Y
AD  - Department of Otorhinolarngology, Head and Neck Surgery, Graduate School of 
      Medicine, Chiba University.
FAU - Nakayama, Satoshi
AU  - Nakayama S
AD  - hermo Fisher Diagnostics.
LA  - jpn
PT  - Journal Article
PL  - Japan
TA  - Arerugi
JT  - Arerugi = [Allergy]
JID - 0241212
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - *Cupressus
MH  - Humans
MH  - Immunoglobulin E
MH  - Japan
MH  - Pollen
MH  - *Rhinitis, Allergic, Seasonal
OTO - NOTNLM
OT  - Cha o 1
OT  - Cha o 2
OT  - ImmunoCAP
OT  - cypress pollen
OT  - specific IgE
EDAT- 2018/02/21 06:00
MHDA- 2019/05/11 06:00
CRDT- 2018/02/21 06:00
PHST- 2018/02/21 06:00 [entrez]
PHST- 2018/02/21 06:00 [pubmed]
PHST- 2019/05/11 06:00 [medline]
AID - 10.15036/arerugi.67.67 [doi]
PST - ppublish
SO  - Arerugi. 2018;67(1):67-71. doi: 10.15036/arerugi.67.67.