PMID- 29459715
OWN - NLM
STAT- MEDLINE
DCOM- 20190916
LR  - 20220408
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Feb 19
TI  - Saliva as a medium to detect and measure biomarkers related to pain.
PG  - 3220
LID - 10.1038/s41598-018-21131-4 [doi]
LID - 3220
AB  - Saliva is often neglected as a body fluid of diagnostic or prognostic value, even 
      though generally well accepted by the patients. This is due to lack of a 
      standardized collection procedure. The aim of this study was to identify the 
      ideal saliva collection technique and develop new sensitive methods to detect and 
      analyse markers related to pain in healthy pain-free subjects. Plasma and five 
      different saliva collection approached was evaluated during strictly controlled 
      conditions. Levels of nerve growth factor (NGF), calcitonin gene-related peptide 
      (CGRP) and brain derived neurotropic factor (BDNF) were determined using novel 
      western blotting based technology. Glutamate and substance P (SP) was determined 
      using commercial available methods. Several new isoforms were found for NGF, CGRP 
      and BDNF in saliva. The isoform pattern showed significant variation in both 
      expression and chemiluminescence levels between different collection methods. New 
      sensitive methods to study pain related markers in saliva were developed in this 
      study. Furthermore, we are first to demonstrate a correlation between the 
      Glutamate concentration in stimulated whole saliva and blood. However, the 
      fundamental conclusion drawn is the importance of consistency in the collection 
      method.
FAU - Jasim, Hajer
AU  - Jasim H
AD  - Division of Oral Diagnostics and Rehabilitation, Department of Dental Medicine 
      and Scandinavian Center for Orofacial neuroscience (SCON), Karolinska Institutet, 
      SE14104, Huddinge, Sweden. hajer.jasim@ki.se.
AD  - Folktandvarden Stockholm AB, Stockholm, Sweden. hajer.jasim@ki.se.
FAU - Carlsson, Anders
AU  - Carlsson A
AD  - Division of Community Medicine, Department of Medical and Health Sciences, 
      Faculty of Health Sciences, Linkoping University and Pain and Rehabilitation 
      Center, Anaesthetics, Operations and Specialty Surgery Center, Region 
      Ostergotland, Linkoping, Sweden.
FAU - Hedenberg-Magnusson, Britt
AU  - Hedenberg-Magnusson B
AD  - Division of Oral Diagnostics and Rehabilitation, Department of Dental Medicine 
      and Scandinavian Center for Orofacial neuroscience (SCON), Karolinska Institutet, 
      SE14104, Huddinge, Sweden.
AD  - Folktandvarden Stockholm AB, Stockholm, Sweden.
FAU - Ghafouri, Bijar
AU  - Ghafouri B
AD  - Division of Community Medicine, Department of Medical and Health Sciences, 
      Faculty of Health Sciences, Linkoping University and Pain and Rehabilitation 
      Center, Anaesthetics, Operations and Specialty Surgery Center, Region 
      Ostergotland, Linkoping, Sweden.
FAU - Ernberg, Malin
AU  - Ernberg M
AD  - Division of Oral Diagnostics and Rehabilitation, Department of Dental Medicine 
      and Scandinavian Center for Orofacial neuroscience (SCON), Karolinska Institutet, 
      SE14104, Huddinge, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180219
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 33507-63-0 (Substance P)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Adult
MH  - Biomarkers/*analysis
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/analysis
MH  - Calcitonin Gene-Related Peptide/analysis
MH  - Female
MH  - Gene Expression
MH  - Glutamic Acid/analysis
MH  - Healthy Volunteers
MH  - Humans
MH  - Luminescence
MH  - Male
MH  - Nerve Growth Factor/analysis
MH  - *Pain
MH  - Plasma/chemistry
MH  - Saliva/*chemistry
MH  - Specimen Handling/methods
MH  - Substance P/analysis
MH  - Young Adult
PMC - PMC5818517
COIS- The authors declare no competing interests.
EDAT- 2018/02/21 06:00
MHDA- 2019/09/17 06:00
PMCR- 2018/02/19
CRDT- 2018/02/21 06:00
PHST- 2017/11/10 00:00 [received]
PHST- 2018/01/30 00:00 [accepted]
PHST- 2018/02/21 06:00 [entrez]
PHST- 2018/02/21 06:00 [pubmed]
PHST- 2019/09/17 06:00 [medline]
PHST- 2018/02/19 00:00 [pmc-release]
AID - 10.1038/s41598-018-21131-4 [pii]
AID - 21131 [pii]
AID - 10.1038/s41598-018-21131-4 [doi]
PST - epublish
SO  - Sci Rep. 2018 Feb 19;8(1):3220. doi: 10.1038/s41598-018-21131-4.