PMID- 29460119
OWN - NLM
STAT- MEDLINE
DCOM- 20180924
LR  - 20181113
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 43
IP  - 4
DP  - 2018 Apr
TI  - Curcumin Inhibits Monocyte Chemoattractant Protein-1 Expression in TNF-alpha induced 
      Astrocytes Through AMPK Pathway.
PG  - 775-784
LID - 10.1007/s11064-018-2479-x [doi]
AB  - Curcumin, a phenolic pigment, plays an inhibitory role in astrocytes activation, 
      a key step for neuropathic pain (NP). The present study aimed to investigate the 
      mechanism behind the therapeutic effect of Curcumin on NP in vitro. Specifically, 
      we investigated the inhibitory effect of Curcumin on tumor necrosis factor-alpha 
      (TNF-alpha)-induced astrocyte migration. We also studied the effects of Curcumin on 
      monocyte chemoattractant protein-1(MCP-1) expression and activity, as well as 
      super oxide dismutase-2 (SOD2) expression and activity in TNF-alpha-induced 
      astrocytes. Additionally, we investigated whether the 
      adenosine-monophosphate-activated protein kinase signaling (AMPK) pathway was 
      involved in this process. Our data demonstrated that Curcumin inhibited 
      TNF-alpha-induced astrocytes migration, decreased MCP-1 expression, and up-regulated 
      SOD2 expression in TNF-alpha-induced astrocytes in vitro. Our study also indicated 
      that this process was mediated through the AMPK signaling pathway, as addition of 
      Curcumin significantly increased the level of phosphorylated AMPK protein. 
      Furthermore, the specific AMPK activator AICAR (5-aminoimidazole-4-carboxamide 
      1-D-ribofuranoside) mimicked the effects of Curcumin, whereas a selective AMPK 
      inhibitor Compound C (also called dorsomorphin) partially blocked its function. 
      These results could shed light on understanding of the molecular basis for the 
      inhibition of Curcumin on MCP-1 expression during the process of astrocyte 
      activation, and provide a molecular mechanism for using Curcumin in neuropathic 
      pain.
FAU - Qin, Xingping
AU  - Qin X
AD  - Department of Physiology, Collaborative Innovation Center for Brain Science, 
      School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu 
      Street, Wuhan, 430071, China.
AD  - Department of Neurosurgery, Renmin Hospital of Wuhan University, 99 Zhang Zhidong 
      Rd, Wuhan, 430060, China.
FAU - Qiao, Haowen
AU  - Qiao H
AD  - Department of Physiology, Collaborative Innovation Center for Brain Science, 
      School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu 
      Street, Wuhan, 430071, China.
FAU - Wu, Songlin
AU  - Wu S
AD  - Department of Geriatrics, Renmin Hospital of Wuhan University, 99 Zhang Zhidong 
      Rd, Wuhan, 430060, China.
FAU - Cheng, Jing
AU  - Cheng J
AD  - Department of Physiology, Collaborative Innovation Center for Brain Science, 
      School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu 
      Street, Wuhan, 430071, China.
FAU - Wan, Qi
AU  - Wan Q
AD  - Department of Physiology, Collaborative Innovation Center for Brain Science, 
      School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu 
      Street, Wuhan, 430071, China. qiwan123@aliyun.com.
AD  - Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, 
      Qingdao, 266071, China. qiwan123@aliyun.com.
FAU - Liu, Renzhong
AU  - Liu R
AD  - Department of Neurosurgery, Renmin Hospital of Wuhan University, 99 Zhang Zhidong 
      Rd, Wuhan, 430060, China. liurenzhong@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180219
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Antioxidants)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antioxidants/pharmacology
MH  - Astrocytes/*drug effects/metabolism
MH  - Cell Survival/drug effects/physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*antagonists & inhibitors/*biosynthesis
MH  - Curcumin/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression
MH  - MAP Kinase Signaling System/*drug effects/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/*toxicity
OTO - NOTNLM
OT  - Adenosine-monophosphate-activated protein kinase
OT  - Astrocytes
OT  - Curcumin
OT  - Monocyte chemoattractant protein-1
OT  - Neuropathic pain
EDAT- 2018/02/21 06:00
MHDA- 2018/09/25 06:00
CRDT- 2018/02/21 06:00
PHST- 2017/10/23 00:00 [received]
PHST- 2018/01/17 00:00 [accepted]
PHST- 2018/01/14 00:00 [revised]
PHST- 2018/02/21 06:00 [pubmed]
PHST- 2018/09/25 06:00 [medline]
PHST- 2018/02/21 06:00 [entrez]
AID - 10.1007/s11064-018-2479-x [pii]
AID - 10.1007/s11064-018-2479-x [doi]
PST - ppublish
SO  - Neurochem Res. 2018 Apr;43(4):775-784. doi: 10.1007/s11064-018-2479-x. Epub 2018 
      Feb 19.