PMID- 29461187
OWN - NLM
STAT- MEDLINE
DCOM- 20180412
LR  - 20191210
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 67
IP  - 4
DP  - 2018 Apr
TI  - Detection of Coxiella burnetii in heart valve sections by fluorescence in situ 
      hybridization.
PG  - 537-542
LID - 10.1099/jmm.0.000704 [doi]
AB  - PURPOSE: Infective endocarditis is a severe and potentially fatal disease. Nearly 
      a third of all cases remain culture-negative, making a targeted and effective 
      antibiotic therapy of patients challenging. In the past years, fluorescence in 
      situ hybridization (FISH) has proven its value for the diagnosis of infective 
      endocarditis, particularly when it is caused by fastidious bacteria. To increase 
      the number of infective endocarditis causing agents, which can be identified by 
      FISH, we designed and optimized a FISH-probe for the specific detection of 
      Coxiella burnetii in heart valve tissue. METHODOLOGY: Even with specific probes 
      the detection and identification of bacteria can be complicated by the high 
      autofluorescence due to calcification of the analysed tissue. To overcome this 
      problem, we developed a protocol to detect C. burnetii by hybridizing, stripping 
      and reprobing the identical section with different species-specific probes 
      repeatedly.Results/Key findings. The newly designed specific FISH probe and the 
      developed protocol exemplarily allowed us to unequivocally identify C. burnetii 
      in tissue sections of a patient with infective endocarditis. CONCLUSION: This 
      method provides an add-on to existing protocols for the unambiguous diagnosis of 
      bacteria directly within tissues or other difficult tissue samples in cases with 
      small sample size and limited sections.
FAU - Aistleitner, Karin
AU  - Aistleitner K
AD  - Bundeswehr Institute of Microbiology, Munich, Germany.
AD  - Present address: Host Parasite Interactions Section, Laboratory of Bacteriology, 
      Rocky Mountain Laboratories, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Hamilton, MT, USA.
FAU - Jeske, Rimma
AU  - Jeske R
AD  - Bundeswehr Institute of Microbiology, Munich, Germany.
FAU - Wolfel, Roman
AU  - Wolfel R
AD  - Bundeswehr Institute of Microbiology, Munich, Germany.
FAU - Wiessner, Alexandra
AU  - Wiessner A
AD  - Biofilmcenter, Deutsches Herzzentrum Berlin, Berlin, Germany.
AD  - Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat 
      Berlin, Humboldt-Universitat zu Berlin, Institute for Microbiology and Hygiene, 
      Germany.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - Biofilmcenter, Deutsches Herzzentrum Berlin, Berlin, Germany.
AD  - Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat 
      Berlin, Humboldt-Universitat zu Berlin, Institute for Microbiology and Hygiene, 
      Germany.
AD  - DZHK (German Centre for Cardiovascular Research), partner site Berlin, Germany.
FAU - Moter, Annette
AU  - Moter A
AD  - Biofilmcenter, Deutsches Herzzentrum Berlin, Berlin, Germany.
AD  - Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat 
      Berlin, Humboldt-Universitat zu Berlin, Institute for Microbiology and Hygiene, 
      Germany.
AD  - DZHK (German Centre for Cardiovascular Research), partner site Berlin, Germany.
FAU - Stoecker, Kilian
AU  - Stoecker K
AD  - Bundeswehr Institute of Microbiology, Munich, Germany.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20180220
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
SB  - IM
MH  - Coxiella burnetii/genetics/*isolation & purification
MH  - Endocarditis, Bacterial/diagnosis/*microbiology
MH  - Heart Valves/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Q Fever/diagnosis/*microbiology
OTO - NOTNLM
OT  - Coxiella
OT  - FISH
OT  - infective endocarditis
EDAT- 2018/02/21 06:00
MHDA- 2018/04/13 06:00
CRDT- 2018/02/21 06:00
PHST- 2018/02/21 06:00 [pubmed]
PHST- 2018/04/13 06:00 [medline]
PHST- 2018/02/21 06:00 [entrez]
AID - 10.1099/jmm.0.000704 [doi]
PST - ppublish
SO  - J Med Microbiol. 2018 Apr;67(4):537-542. doi: 10.1099/jmm.0.000704. Epub 2018 Feb 
      20.