PMID- 29462204
OWN - NLM
STAT- MEDLINE
DCOM- 20180309
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 2
DP  - 2018
TI  - Microbial diversity and component variation in Xiaguan Tuo Tea during pile 
      fermentation.
PG  - e0190318
LID - 10.1371/journal.pone.0190318 [doi]
LID - e0190318
AB  - Xiaguan Tuo Tea is largely consumed by the Chinese, but there is little research 
      into the microbial diversity and component changes during the fermentation of 
      this tea. In this study, we first used fluorescence in situ hybridization (FISH), 
      next-generation sequencing (NGS) and chemical analysis methods to determine the 
      microbial abundance and diversity and the chemical composition during 
      fermentation. The FISH results showed that the total number of microorganisms 
      ranges from 2.3x102 to 4.0x108 cells per gram of sample during fermentation and 
      is mainly dominated by fungi. In the early fermentation stages, molds are 
      dominant (0.6x102~2.8x106 cells/g, 0~35 d). However, in the late stages of 
      fermentation, yeasts are dominant (3.6x104~9.6x106 cells/g, 35~56 d). The 
      bacteria have little effect during the fermentation of tea (102~103 cells/g, <1% 
      of fungus values). Of these fungi, A. niger (Aspergillus niger) and B. 
      adeninivorans (Blastobotrys adeninivorans) are identified as the two most common 
      strains, based on Next-generation Sequencing (NGS) analysis. Peak diversity in 
      tea was observed at day 35 of fermentation (Shannon-Weaver index: 1.195857), and 
      lower diversity was observed on days 6 and 56 of fermentation (Shannon-Weaver 
      index 0.860589 and 1.119106, respectively). During the microbial fermentation, 
      compared to the unfermented tea, the tea polyphenol content decreased by 54%, and 
      the caffeine content increased by 59%. Theanine and free amino acid contents were 
      reduced during fermentation by 81.1 and 92.85%, respectively.
FAU - Li, Haizhou
AU  - Li H
AUID- ORCID: 0000-0002-2050-6645
AD  - Research Center for Translational Medicine at Shanghai East Hospital, School of 
      Life Sciences and Technology, Tongji University, Shanghai, China.
FAU - Li, Min
AU  - Li M
AD  - Research Center for Translational Medicine at Shanghai East Hospital, School of 
      Life Sciences and Technology, Tongji University, Shanghai, China.
FAU - Yang, Xinrui
AU  - Yang X
AD  - Research Center for Translational Medicine at Shanghai East Hospital, School of 
      Life Sciences and Technology, Tongji University, Shanghai, China.
FAU - Gui, Xin
AU  - Gui X
AD  - Research Center for Translational Medicine at Shanghai East Hospital, School of 
      Life Sciences and Technology, Tongji University, Shanghai, China.
FAU - Chen, Guofeng
AU  - Chen G
AD  - Yunnan Xiaguan Tuo Tea (Group) Co., Ltd, Dali, Yunnan, China.
FAU - Chu, Jiuyun
AU  - Chu J
AD  - Yunnan Xiaguan Tuo Tea (Group) Co., Ltd, Dali, Yunnan, China.
FAU - He, Xingwang
AU  - He X
AD  - Yunnan Xiaguan Tuo Tea (Group) Co., Ltd, Dali, Yunnan, China.
FAU - Wang, Weitao
AU  - Wang W
AD  - Yunnan Xiaguan Tuo Tea (Group) Co., Ltd, Dali, Yunnan, China.
FAU - Han, Feng
AU  - Han F
AD  - College of Architecture and Urban Planning, Tongji University, Shanghai, China.
FAU - Li, Ping
AU  - Li P
AUID- ORCID: 0000-0002-1417-6360
AD  - Research Center for Translational Medicine at Shanghai East Hospital, School of 
      Life Sciences and Technology, Tongji University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180220
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Tea)
SB  - IM
MH  - Bacteria/isolation & purification
MH  - China
MH  - Colony Count, Microbial
MH  - *Fermentation
MH  - Fungi/isolation & purification
MH  - High-Throughput Nucleotide Sequencing
MH  - In Situ Hybridization, Fluorescence
MH  - Tea/metabolism/*microbiology
PMC - PMC5819769
COIS- Competing Interests: The authors [GC, JC, XH, WW] were employed in Yunnan Xiaguan 
      Tuo Tea (Group) Co., Ltd. This does not alter our adherence to PLOS ONE policies 
      on sharing data and materials.
EDAT- 2018/02/21 06:00
MHDA- 2018/03/10 06:00
PMCR- 2018/02/20
CRDT- 2018/02/21 06:00
PHST- 2017/07/26 00:00 [received]
PHST- 2017/12/12 00:00 [accepted]
PHST- 2018/02/21 06:00 [entrez]
PHST- 2018/02/21 06:00 [pubmed]
PHST- 2018/03/10 06:00 [medline]
PHST- 2018/02/20 00:00 [pmc-release]
AID - PONE-D-17-26001 [pii]
AID - 10.1371/journal.pone.0190318 [doi]
PST - epublish
SO  - PLoS One. 2018 Feb 20;13(2):e0190318. doi: 10.1371/journal.pone.0190318. 
      eCollection 2018.