PMID- 29462613
OWN - NLM
STAT- MEDLINE
DCOM- 20180509
LR  - 20180509
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 497
IP  - 2
DP  - 2018 Mar 4
TI  - Chlamydia pneumoniae infection promotes monocyte transendothelial migration by 
      increasing vascular endothelial cell permeability via the tyrosine 
      phosphorylation of VE-cadherin.
PG  - 742-748
LID - S0006-291X(18)30382-6 [pii]
LID - 10.1016/j.bbrc.2018.02.145 [doi]
AB  - Migration of monocytes into the subendothelial layer of the intima is one of the 
      critical events in early atherosclerosis. Chlamydia pneumoniae (C. pneumoniae) 
      infection has been shown to promote monocyte transendothelial migration (TEM). 
      However, the exact mechanisms have not yet been fully clarified. In this study, 
      we tested the hypothesis that C. pneumoniae infection increases vascular 
      endothelial cell (VEC) permeability and subsequent monocyte TEM through 
      stimulating the tyrosine phosphorylation of vascular endothelial-cadherin 
      (VE-cadherin). Here, we demonstrated that C. pneumoniae infection promoted 
      monocyte TEM in a TEM assay possibly by increasing the permeability of a VEC line 
      EA.hy926 cell as assessed by measuring the passage of FITC-BSA across a VEC 
      monolayer. Subsequently, Western blot analysis showed that C. pneumoniae 
      infection induced VE-cadherin internalization. Our further data revealed that 
      Src-mediated VE-cadherin phosphorylation at Tyr658 was involved in C. pneumoniae 
      infection-induced internalization of VE-cadherin, VEC hyperpermeability and 
      monocyte TEM. Taken together, our data indicate that C. pneumoniae infection 
      promotes monocyte TEM by increasing VEC permeability via the tyrosine 
      phosphorylation and internalization of VE-cadherin in VECs.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Liu, Jingya
AU  - Liu J
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Miao, Guolin
AU  - Miao G
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Wang, Beibei
AU  - Wang B
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Zheng, Ningbo
AU  - Zheng N
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Ma, Lu
AU  - Ma L
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Chen, Xiaoyu
AU  - Chen X
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Wang, Guangyan
AU  - Wang G
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Zhao, Xi
AU  - Zhao X
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China.
FAU - Zhang, Lijun
AU  - Zhang L
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China. Electronic address: zhanglj@tmu.edu.cn.
FAU - Zhang, Lijun
AU  - Zhang L
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 
      300070, China. Electronic address: zhanglijun@tmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180217
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, CD)
RN  - 0 (Cadherins)
RN  - 0 (cadherin 5)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Cadherins/*metabolism
MH  - *Capillary Permeability
MH  - Cells, Cultured
MH  - Chlamydophila Infections/*metabolism/microbiology/pathology
MH  - Chlamydophila pneumoniae/*physiology
MH  - Endothelium, Vascular/metabolism/microbiology/pathology
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Monocytes/cytology/*microbiology/pathology
MH  - Phosphorylation
MH  - *Transendothelial and Transepithelial Migration
OTO - NOTNLM
OT  - Chlamydia pneumoniae
OT  - Monocyte
OT  - Transendothelial migration
OT  - Tyrosine phosphorylation
OT  - VE-cadherin internalization
OT  - Vascular endothelial cell permeability
EDAT- 2018/02/21 06:00
MHDA- 2018/05/10 06:00
CRDT- 2018/02/21 06:00
PHST- 2018/02/15 00:00 [received]
PHST- 2018/02/16 00:00 [accepted]
PHST- 2018/02/21 06:00 [pubmed]
PHST- 2018/05/10 06:00 [medline]
PHST- 2018/02/21 06:00 [entrez]
AID - S0006-291X(18)30382-6 [pii]
AID - 10.1016/j.bbrc.2018.02.145 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Mar 4;497(2):742-748. doi: 
      10.1016/j.bbrc.2018.02.145. Epub 2018 Feb 17.