PMID- 29462699
OWN - NLM
STAT- MEDLINE
DCOM- 20180907
LR  - 20180907
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 217
DP  - 2018 May 10
TI  - Effect of Eucommia ulmoides Oliv., Gynostemma pentaphyllum (Thunb.) Makino, and 
      Curcuma longa L. on Th1- and Th2-cytokine responses and human leukocyte 
      antigen-DR expression in peripheral blood mononuclear cells of septic patients.
PG  - 195-204
LID - S0378-8741(17)32678-8 [pii]
LID - 10.1016/j.jep.2018.02.024 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Many traditional Chinese medicines (TCM), such as 
      Eucommia ulmoides Oliv., Gynostemma pentaphyllum (Thunb.) Makino, and Curcuma 
      longa L., have been reported to have various immune-modulatory effects. AIM OF 
      THE STUDY: To determine the effects of extracts from these three TCM on type 1 T 
      help (Th1)- and Th2-cytokine responses and human leukocyte antigen (HLA)-DR 
      expression in peripheral blood mononuclear cells (PBMCs) obtained from septic 
      patients. MATERIALS AND METHODS: Lipopolysaccharide (LPS)-stimulated PBMCs of 
      healthy controls and septic patients were cultured for 48 hs with or without 
      0.05/0.1 mg/ml of TCM extract. HLA-DR expression in monocytes was detected using 
      flow cytofluorimetry. The interferon [IFN]-gamma, tumor necrosis factor [TNF]-alpha, 
      interleukin (IL)- 2, IL-5, IL-10, and IL-13 levels in supernatants were measured 
      with a human enzyme-linked immunosorbent assay. RESULTS: Treatment with either 
      0.05 or 0.1 mg/ml of C. longa L. extract significantly restored the percentage of 
      HLA-DR-positive monocytes, which was decreased by LPS in control and patient 
      groups. Treatment with 0.05 or 0.1 mg/ml E. ulmoides Oliv. and C.longa L. extract 
      decreased IL-10 production from LPS-stimulated PBMCs of controls and patients. In 
      patients with sepsis, C. longa L. extract decreased IL-10 production to a greater 
      degree than did E. ulmoides Oliv extract. Although IFN-gamma, TNF-alpha, or IL-13 
      productions from LPS-stimulated PBMCs were influenced by E. ulmoides Oliv., G. 
      pentaphyllum (Thunb.) Makino, or C. longa L. in control or sepsis groups in this 
      study, only the influence of IL-10 was consistent in both control and sepsis 
      groups. CONCLUSIONS: By enhancing monocyte HLA-DR expression and decreasing IL-10 
      production, C. longa L. might help restore inflammatory responses in septic 
      patients to eradicate pathogens.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Wu, Huang-Pin
AU  - Wu HP
AD  - Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial 
      Hospital, Keelung 204, Taiwan; Chang Gung University College of Medicine, Taoyuan 
      333, Taiwan. Electronic address: whanpyng@cgmh.org.tw.
FAU - Lin, Yin-Ku
AU  - Lin YK
AD  - Chang Gung University College of Medicine, Taoyuan 333, Taiwan; Department of 
      Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung 204, Taiwan. 
      Electronic address: lin1266@cgmh.org.tw.
LA  - eng
PT  - Journal Article
DEP - 20180217
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Immunologic Factors)
RN  - 0 (Plant Extracts)
RN  - 856YO1Z64F (turmeric extract)
SB  - IM
MH  - Aged
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - *Curcuma/chemistry
MH  - Cytokines/immunology/*metabolism
MH  - *Eucommiaceae/chemistry
MH  - Female
MH  - *Gynostemma/chemistry
MH  - HLA-DR Antigens/immunology/*metabolism
MH  - Humans
MH  - Immunologic Factors/*pharmacology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*drug effects/immunology/metabolism
MH  - Phytotherapy
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Plants, Medicinal
MH  - Sepsis/blood/*immunology
MH  - Th1 Cells/*drug effects/immunology/metabolism
MH  - Th1-Th2 Balance/*drug effects
MH  - Th2 Cells/*drug effects/immunology/metabolism
OTO - NOTNLM
OT  - Curcuma longa L.
OT  - Human leukocyte antigen-DR
OT  - Interleukin-10
OT  - Sepsis
EDAT- 2018/02/21 06:00
MHDA- 2018/09/08 06:00
CRDT- 2018/02/21 06:00
PHST- 2017/07/16 00:00 [received]
PHST- 2018/02/14 00:00 [revised]
PHST- 2018/02/16 00:00 [accepted]
PHST- 2018/02/21 06:00 [pubmed]
PHST- 2018/09/08 06:00 [medline]
PHST- 2018/02/21 06:00 [entrez]
AID - S0378-8741(17)32678-8 [pii]
AID - 10.1016/j.jep.2018.02.024 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2018 May 10;217:195-204. doi: 10.1016/j.jep.2018.02.024. Epub 
      2018 Feb 17.