PMID- 29463827
OWN - NLM
STAT- MEDLINE
DCOM- 20190916
LR  - 20190916
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Feb 20
TI  - Long non-coding RNA AC026166.2-001 inhibits cell proliferation and migration in 
      laryngeal squamous cell carcinoma by regulating the miR-24-3p/p27 axis.
PG  - 3375
LID - 10.1038/s41598-018-21659-5 [doi]
LID - 3375
AB  - Long non-coding RNA (lncRNA) AC026166.2-001 was found to be down-regulated in 
      laryngeal squamous cell carcinoma (LSCC) tissues and metastatic neck lymph nodes. 
      Decreased AC026166.2-001 was associated with poorer prognosis and may act as a 
      novel biomarker for LSCC patients. In this study, AC026166.2-001 was 
      overexpressed by a lentivirus vector and down-regulated by a small interfering 
      RNA (siRNA). The results of real-time cell analysis (RTCA) and a plate colony 
      formation assay showed that AC026166.2-001 inhibited LSCC cell proliferation and 
      the clone-forming capacity. Cell cycle distribution and related protein changes 
      were measured by flow cytometry. AC026166.2-001 arrested the cell cycle at the G1 
      phase and induced apoptosis. In addition, AC026166.2-001 decreased cell migration 
      as measured by wound healing assays and transwell migration assays. Moreover, 
      luciferase reporter assay and Western blotting results suggested that 
      AC026166.2-001 acts as a sponge of miR-24-3p and regulates the expression of p27 
      and cyclin D1. The in vivo results showed that AC026166.2-001 significantly 
      suppressed the growth of LSCC xenografts and promoted apoptosis. We validated the 
      molecular mechanisms underlying AC026166.2-001 in LSCC. This is the first report 
      of AC026166.2-001 acting as a tumor suppressor in LSCC by regulating the 
      miR-24-3p/p27 axis.
FAU - Shen, Zhisen
AU  - Shen Z
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China. 
      szs7216@163.com.
FAU - Hao, Wenjuan
AU  - Hao W
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
FAU - Zhou, Chongchang
AU  - Zhou C
AUID- ORCID: 0000-0002-8728-6819
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
FAU - Deng, Hongxia
AU  - Deng H
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
FAU - Ye, Dong
AU  - Ye D
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
FAU - Li, Qun
AU  - Li Q
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
FAU - Lin, Lexi
AU  - Lin L
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
FAU - Cao, Bing
AU  - Cao B
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, the Affiliated 
      Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
FAU - Guo, Junming
AU  - Guo J
AD  - Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of 
      Pathophysiology, Medical School of Ningbo University, Ningbo, 315211, China. 
      guojunming@nbu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180220
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (MIRN24 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (p27 antigen)
SB  - IM
MH  - Apoptosis
MH  - Carcinoma, Squamous Cell/*physiopathology
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Cytological Techniques
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - Laryngeal Neoplasms/*physiopathology
MH  - MicroRNAs/*metabolism
MH  - Models, Biological
MH  - Proliferating Cell Nuclear Antigen/*metabolism
MH  - RNA, Long Noncoding/*metabolism
PMC - PMC5820272
COIS- The authors declare no competing interests.
EDAT- 2018/02/22 06:00
MHDA- 2019/09/17 06:00
PMCR- 2018/02/20
CRDT- 2018/02/22 06:00
PHST- 2017/09/07 00:00 [received]
PHST- 2018/02/06 00:00 [accepted]
PHST- 2018/02/22 06:00 [entrez]
PHST- 2018/02/22 06:00 [pubmed]
PHST- 2019/09/17 06:00 [medline]
PHST- 2018/02/20 00:00 [pmc-release]
AID - 10.1038/s41598-018-21659-5 [pii]
AID - 21659 [pii]
AID - 10.1038/s41598-018-21659-5 [doi]
PST - epublish
SO  - Sci Rep. 2018 Feb 20;8(1):3375. doi: 10.1038/s41598-018-21659-5.